HER2/CD340 蛋白, Rat (His)

The HER2/CD340 protein is a key protein tyrosine kinase that is a component of multiple cell surface receptor complexes and requires coreceptors for ligand binding. Its interaction within the neuregulin-receptor complex depends on neuregulin, and GP30 emerges as a potential ligand. HER2/CD340 Protein, Rat (His) is the recombinant rat-derived HER2/CD340 protein, expressed by E. coli , with C-6*His labeled tag. The total length of HER2/CD340 Protein, Rat (His) is 257 a.a., with molecular weight of ~32 kDa.

HER2/CD340 蛋白是一种蛋白酪氨酸激酶，是各种细胞表面受体复合物中的关键成分，需要一个辅助受体来进行配体结合。虽然它是神经调节蛋白-受体复合物中的重要元素，但它特别需要神经调节蛋白的存在才能相互作用。此外，GP30 是该受体的潜在配体。除了其受体功能外，HER2 在外周微管 (MT) 的调节中发挥着关键作用，影响其生长和稳定。激活后，ERBB2 诱导的 MEMO1-RHOA-DIAPH1 信号通路磷酸化并抑制细胞膜上的 GSK3B，从而阻止 APC 和 CLASP2 的磷酸化。这反过来又促进了膜结合 APC 与细胞膜的结合，从而实现了对微管捕获和稳定至关重要的 MACF1 的定位。此外，HER2 表现出多种相互作用，包括其在细胞膜上优先与 CPNE3 的酪氨酸磷酸化形式结合，特别是以生长因子调蛋白依赖性方式结合。在细胞核中，HER2 将其影响延伸至转录调控，与 PTGS2/COX-2 启动子中的特定序列关联并激活转录。它还参与 CDKN1A 的转录激活，这是一个涉及 STAT3 和 SRC 的过程，通过 RNA Pol I 促进 rRNA 基因转录，并增强蛋白质合成和细胞生长。HER2 的多方面作用凸显了它在细胞过程中的复杂参与，从膜动力学到转录调节。

The enzyme activity of this recombinant protein is testing in progress, we cannot offer a guarantee yet.

Rat

E. coli

C-6*His

P06494 (A67-V323)

24337  [NCBI]

ANASLSFLQDIQEVQGYMLIAHNQVKRVPLQRLRIVRGTQLFEDKYALAVLDNRDPQDNVAASTPGRTPEGLRELQLRSLTEILKGGVLIRGNPQLCYQDMVLWKDVFRKNNQLAPVDIDTNRSRACPPCAPACKDNHCWGESPEDCQILTGTICTSGCARCKGRLPTDCCHEQCAAGCTGPKHSDCLACLHFNHSGICELHCPALVTYNTDTFESMHNPEGRYTFGASCVTTCPYNYLSTEVGSCTLVCPPNNQEV

Approximately 32 kDa

Greater than 95% as determined by reducing SDS-PAGE.

无菌粉末.

Lyophilized from a 0.2 μm filtered solution of PBS, 5% Trehalose, 4M Urea, pH 7.4.

<1 EU/μg, determined by LAL method.

It is not recommended to reconstitute to a concentration less than 100 μg/mL in ddH₂O.

Stored at -20°C for 2 years. After reconstitution, it is stable at 4°C for 1 week or -20°C for longer (with carrier protein). It is recommended to freeze aliquots at -20°C or -80°C for extended storage.

Room temperature in continental US; may vary elsewhere.