1. 重组蛋白
  2. CD Antigens Receptor Proteins
  3. Epithelial cell CD Proteins Endothelial cell CD Proteins G-Protein-Coupled Receptors (GPCRs)
  4. Frizzled-4/CD344 Frizzled
  5. Frizzled-4
  6. Frizzled-4/CD344 蛋白, Human (HEK293, Fc)

Frizzled-4/CD344 蛋白, Human (HEK293, Fc)

目录号: HY-P74141
产品使用指南

Frizzled-4/CD344 蛋白是 Wnt 蛋白的受体,与经典的 β-连环蛋白途径相关,可激活蓬乱的蛋白、抑制 GSK-3 激酶并触发 Wnt 靶基因。在视网膜血管化中,它充当 Wnt 蛋白和诺里蛋白 (NDP) 的受体,刺激 β-连环蛋白积累和 LEF/TCF 介导的转录。Frizzled-4/CD344 蛋白, Human (HEK293, Fc) 是重组的 Frizzled-4/CD344 蛋白,由 HEK293 表达,带有 C-hFc 标签。Frizzled-4/CD344 蛋白, Human (HEK293, Fc) 全长 180 个氨基酸,分子量约为 ~43.3 kDa。

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     可免费申领三个不同产品的试用装。

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描述

The Frizzled-4/CD344 protein is a receptor for Wnt proteins and is associated with the classical β-catenin pathway, activating disheveled proteins, inhibiting GSK-3 kinase, and triggering Wnt target genes. In retinal vascularization, it acts as a receptor for Wnt proteins and Norrin (NDP), stimulating β-catenin accumulation and LEF/TCF-mediated transcription. Frizzled-4/CD344 Protein, Human (HEK293, Fc) is the recombinant human-derived Frizzled-4/CD344 protein, expressed by HEK293 , with C-hFc labeled tag. The total length of Frizzled-4/CD344 Protein, Human (HEK293, Fc) is 180 a.a., with molecular weight of ~43.3 kDa.

研究背景

Frizzled-4/CD344 蛋白作为 Wnt 蛋白的受体,特别与经典 β-连环蛋白信号通路相关,涉及蓬乱蛋白的激活、GSK-3 激酶的抑制、β-连环蛋白的核积累以及随后的Wnt 靶基因。在视网膜血管化的背景下,Frizzled-4 通过充当 Wnt 蛋白和诺里蛋白 (NDP) 的受体发挥着关键作用,促进 β-catenin 积累并刺激 LEF/TCF 介导的转录程序。尽管在一些家族成员中观察到以 PKC 和钙通量为特征的次级信号传导途径,但其与经典途径的整合,特别是在 Wnt 介导的 GSK-3 激酶失活的背景下,仍然不确定。通过观察到与 MAGI3、NDP、TSKU 和磷脂酰肌醇蛋白聚糖 GPC3 的相互作用,表明 Frizzled-4 参与组织形态发生和极性信息的细胞间传递,突出了其在细胞过程和信号级联中的复杂作用。

种属

Human

表达系统

HEK293

标签

C-hFc

蛋白编号

Q9ULV1 (M1-E180)

基因 ID
蛋白结构
N-term
FZD4 (M1-E180)
Accession # Q9ULV1
hFc
C-term
同用名
CD344 antigen; CD344; EVR1; FEVR; Frizzled-4; Fz-4; FZD4
分子量

Approximately 43.3 kDa

纯度

Greater than 95% as determined by reducing SDS-PAGE.

内毒素含量

<1 EU/μg, determined by LAL method.

文件资料
Help & FAQs
  • Do most proteins show cross-species activity?

    Species cross-reactivity must be investigated individually for each product. Many human cytokines will produce a nice response in mouse cell lines, and many mouse proteins will show activity on human cells. Other proteins may have a lower specific activity when used in the opposite species.

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The reconstitution calculator equation

Volume (to add to vial) = Mass (in vial) ÷ Desired Reconstitution Concentration

Volume (to add to vial) = Mass (in vial) ÷ Desired Reconstitution Concentration
= ÷

The dilution calculator equation

Concentration (start) × Volume (start) = Concentration (final) × Volume (final)

This equation is commonly abbreviated as: C1V1 = C2V2

浓度 (start) × 体积 (start) = 浓度 (final) × 体积 (final)
× = ×
C1   V1   C2   V2

The specific activity calculator equation

Specific Activity (Unit/mg) = 106 ÷ Biological Activity (ED50)

Specific Activity (Unit/mg) = 106 ÷ Biological Activity (ED50)
Unit/mg = 106 ÷ ng/mL

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产品名称:
Frizzled-4/CD344 Protein, Human (HEK293, Fc)
目录号:
HY-P74141
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