Frizzled-6 蛋白, Human (HEK293, His)

Frizzled-6 is a Wnt receptor that complexly activates signaling pathways that converge primarily on the β-catenin canonical pathway. This triggers disorganized protein activation, GSK-3 kinase inhibition, nuclear β-catenin accumulation, and Wnt target gene activation. Frizzled-6 Protein, Human (HEK293, His) is the recombinant human-derived Frizzled-6 protein, expressed by HEK293 , with C-His labeled tag. The total length of Frizzled-6 Protein, Human (HEK293, His) is 135 a.a., with molecular weight of ~20-28 kDa.

Frizzled-6 是 Wnt 蛋白的受体，复杂地参与主要汇聚于 β-连环蛋白经典途径的信号传导途径。该级联触发了杂乱蛋白的激活、GSK-3 激酶的抑制、β-连环蛋白的核积累以及随后 Wnt 靶基因的激活。虽然在一些家族成员中观察到涉及 PKC 和钙通量的次级信号通路，但其与经典通路的精确关系仍不清楚，并且 PKC 对 Wnt 介导的 GSK-3 激酶失活的要求增加了这种相互作用的复杂性。与 G 蛋白的相互作用是这两条途径的组成部分。Frizzled-6 在组织形态发生和分化组织中的极性信息转导中发挥着关键作用。它与 FZD3 配合，有助于神经管闭合并调节平面细胞极性的建立，特别是在内耳内选定的听觉和前庭感觉细胞的顶面上定向不对称的静纤毛束。与 LMBR1L 的相互作用进一步强调了其功能。

Measured by its binding ability in a functional ELISA. Immobilized Human Frizzled-6, at 0.1μg/mL (100 μL/well) can bind Wnt-5a. The ED₅₀ for this effect is 125.3 ng/mL.

Human

HEK293

C-6*His

O60353-1/NP_003497.2 (H19-V153)

8323  [NCBI]

HSLFTCEPITVPRCMKMAYNMTFFPNLMGHYDQSIAAVEMEHFLPLANLECSPNIETFLCKAFVPTCIEQIHVVPPCRKLCEKVYSDCKKLIDTFGIRWPEELECDRLQYCDETVPVTFDPHTEFLGPQKKTEQV

Approximately 20-28 kDa due to the glycosylation

Greater than 95% as determined by reducing SDS-PAGE.

无菌粉末.

Lyophilized from a 0.2 μm filtered solution of PBS, pH 7.4.

<1 EU/μg, determined by LAL method.

It is not recommended to reconstitute to a concentration less than 100 μg/mL in ddH₂O. For long term storage it is recommended to add a carrier protein (0.1% BSA, 5% HSA, 10% FBS or 5% Trehalose).

Stored at -20°C for 2 years. After reconstitution, it is stable at 4°C for 1 week or -20°C for longer (with carrier protein). It is recommended to freeze aliquots at -20°C or -80°C for extended storage.

Room temperature in continental US; may vary elsewhere.