Frizzled-7 蛋白, Human (CHO, hFc)

GIP Protein is a protein involved in the regulation of glucose homeostasis and insulin secretion. It plays a crucial role in modulating pancreatic beta-cell function and glucose metabolism. Dysregulation of GIP Protein has been associated with various diseases, including type 2 diabetes and obesity. Targeting GIP Protein may offer potential therapeutic interventions in these conditions by improving glucose regulation and insulin sensitivity. Frizzled-7 Protein, Human (CHO, hFc) is the recombinant human-derived Frizzled-7 protein, expressed by CHO , with C-hFc labeled tag. The total length of Frizzled-7 Protein, Human (CHO, hFc) is 153 a.a., with molecular weight of 46-60 kDa.

Frizzled-7 (FZD7) 蛋白是“卷曲”基因家族的成员，编码 Wnt 信号蛋白的 7 跨膜结构域受体。FZD7 蛋白结构包括 N 端信号序列、富含半胱氨酸的胞外结构域（具有 Fz 家族成员特有的 10 个半胱氨酸残基）、7 个假定的跨膜结构域以及具有 PDZ 结构域结合基序的胞内 C 端尾部。FZD7 基因的表达可能会下调 APC 功能并增强 β-连环蛋白介导的信号，特别是在低分化的人类食管癌中观察到。这凸显了 FZD7 在调节关键细胞信号传导途径中的作用，特别是在癌症进展的背景下。

Measured by its binding ability in a functional ELISA. Immobilized Human Frizzled-7, at 0.1μg/mL (100 μL/well) can bind Biotinylated Wnt-5a. The ED₅₀ for this effect is 257.8 ng/mL.

Human

CHO

C-hFc

O75084/NP_003498 (Q33-L185)

8324  [NCBI]

QPYHGEKGISVPDHGFCQPISIPLCTDIAYNQTILPNLLGHTNQEDAGLEVHQFYPLVKVQCSPELRFFLCSMYAPVCTVLDQAIPPCRSLCERARQGCEALMNKFGFQWPERLRCENFPVHGAGEICVGQNTSDGSGGPGGGPTAYPTAPYL

46-60 kDa

• 
  Greater than 95% as determined by reducing SDS-PAGE.

无菌粉末.

Lyophilized from a 0.2 μm filtered solution of 20 mM PB, 150 mM NaCl, pH 7.4.

<1 EU/μg, determined by LAL method.

It is not recommended to reconstitute to a concentration less than 200 μg/mL in ddH₂O. For long term storage it is recommended to add a carrier protein (0.1% BSA, 5% HSA, 10% FBS or 5% Trehalose).

Stored at -20°C for 2 years. After reconstitution, it is stable at 4°C for 1 week or -20°C for longer (with carrier protein). It is recommended to freeze aliquots at -20°C or -80°C for extended storage.

Room temperature in continental US; may vary elsewhere.