FRZB 蛋白, Human (HEK293, His)

FRZB protein is a soluble Frizzled-related protein (sFRP) that modulates Wnt signaling by directly interacting with Wnt, thereby regulating cell growth and differentiation. FRZB, also known as SFRP3, plays a key role in limb skeletogenesis as a Wnt8 signaling antagonist. FRZB Protein, Human (HEK293, His) is the recombinant human-derived FRZB protein, expressed by HEK293 , with C-His labeled tag. The total length of FRZB Protein, Human (HEK293, His) is 294 a.a., with molecular weight of ~41 kDa.

FRZB 是一种可溶性卷曲相关蛋白 (sFRP)，通过与 Wnt 的直接相互作用在调节 Wnt 信号传导中发挥关键作用，有助于调节特定细胞类型的细胞生长和分化。特别是，FRZB（也称为 SFRP3）在肢体骨骼发生中发挥着至关重要的作用，作为 Wnt8 信号传导的拮抗剂。其调节影响延伸到软骨细胞成熟和长骨发育的精确协调。除了参与 Wnt 信号传导调节之外，FRZB 还参与分子相互作用，例如它与 MYOC 的相互作用，从而突出了其在细胞过程中的多样化功能和重要性。FRZB 的多方面调节作用强调了其在 Wnt 介导的途径和骨骼发生的复杂景观中的重要性。

Measured by its ability to inhibit proliferation of HeLa human cervical epithelial carcinoma cells. The ED₅₀ for this effect is 0.359 μg/mL, corresponding to a specific activity is 2.786×10³ units/mg.

Human

HEK293

C-His

Q92765 (A32-N325)

2487  [NCBI]

AAACEPVRIPLCKSLPWNMTKMPNHLHHSTQANAILAIEQFEGLLGTHCSPDLLFFLCAMYAPICTIDFQHEPIKPCKSVCERARQGCEPILIKYRHSWPENLACEELPVYDRGVCISPEAIVTADGADFPMDSSNGNCRGASSERCKCKPIRATQKTYFRNNYNYVIRAKVKEIKTKCHDVTAVVEVKEILKSSLVNIPRDTVNLYTSSGCLCPPLNVNEEYIIMGYEDEERSRLLLVEGSIAEKWKDRLGKKVKRWDMKLRHLGLSKSDSSNSDSTQSQKSGRNSNPRQARN

Approximately 41 kDa

Greater than 85% as determined by reducing SDS-PAGE

无菌粉末

Lyophilized from a 0.2 μm filtered solution of PBS, pH 7.4.

<1 EU/μg, determined by LAL method.

It is not recommended to reconstitute to a concentration less than 100 μg/mL in ddH₂O. For long term storage it is recommended to add a carrier protein (0.1% BSA, 5% HSA, 10% FBS or 5% Trehalose).

Stored at -20°C for 2 years. After reconstitution, it is stable at 4°C for 1 week or -20°C for longer (with carrier protein). It is recommended to freeze aliquots at -20°C or -80°C for extended storage.

Room temperature in continental US; may vary elsewhere.