1. 重组蛋白
  2. Receptor Proteins
  3. G-Protein-Coupled Receptors (GPCRs)
  4. Frizzled
  5. Frizzled-2
  6. FZD2 蛋白, Mouse (HEK293, Fc)

FZD2 蛋白, Mouse (HEK293, Fc)

目录号: HY-P70918
产品使用指南

FZD2 是一种 Wnt 受体,主要激活 β-catenin 经典途径,涉及蓬乱蛋白、GSK-3 激酶抑制、核 β-catenin 积累和 Wnt 靶基因激活。尽管一些家族成员表现出第二 PKC 和钙通量途径,但与经典途径的精确分化和整合仍不清楚。FZD2 蛋白, Mouse (HEK293, Fc) 是重组的 FZD2 蛋白,由 HEK293 表达,带有 C-hFc 标签。FZD2 蛋白, Mouse (HEK293, Fc) 全长 140 个氨基酸,分子量为 55-60 kDa。

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描述

FZD2 is a Wnt receptor that primarily activates the β-catenin classical pathway, involving disheveled protein, GSK-3 kinase inhibition, nuclear β-catenin accumulation, and Wnt target gene activation. Although some family members exhibit second PKC and calcium flux pathways, the precise differentiation and integration with the canonical pathways remains unclear. FZD2 Protein, Mouse (HEK293, Fc) is the recombinant mouse-derived FZD2 protein, expressed by HEK293 , with C-hFc labeled tag. The total length of FZD2 Protein, Mouse (HEK293, Fc) is 140 a.a., with molecular weight of 55-60 kDa.

研究背景

FZD2 作为 Wnt 蛋白的受体,主要与 β-catenin 经典信号通路相关,协调蓬乱蛋白的激活、GSK-3 激酶的抑制、β-catenin 的核积累以及 Wnt 靶基因的激活。虽然某些家族成员表现出涉及 PKC 和钙通量的第二种信号传导途径,但与经典途径的精确区别和潜在整合仍不清楚,PKC 似乎对于 Wnt 介导的 GSK-3 激酶失活至关重要。两种途径都需要与 G 蛋白相互作用。FZD2 可能参与组织形态发生和/或分化组织期间极性信息的转导,进一步强调了其在细胞过程中的复杂作用。

种属

Mouse

表达系统

HEK293

标签

C-hFc

蛋白编号

Q9JIP6 (Q29-L168)

基因 ID

57265  [NCBI]

蛋白结构
N-term
FZD2 (Q29-L168)
Accession # Q9JIP6
hFc
C-term
同用名
Frizzled-2; Fz-2; mFz2; Fzd2; frizzled (Drosophila) homolog 2
氨基酸序列

QFHGEKGISIPDHGFCQPISIPLCTDIAYNQTIMPNLLGHTNQEDAGLEVHQFYPLVKVQCSPELRFFLCSMYAPVCTVLEQAIPPCRSICERARQGCEALMNKFGFQWPERLRCEHFPRHGAEQICVGQNHSEDGAPAL

分子量

55-60 kDa

纯度

Greater than 95% as determined by reducing SDS-PAGE.

内毒素含量

<1 EU/μg, determined by LAL method.

文件资料

FZD2 Protein, Mouse (HEK293, Fc) 相关分类

Help & FAQs
  • Do most proteins show cross-species activity?

    Species cross-reactivity must be investigated individually for each product. Many human cytokines will produce a nice response in mouse cell lines, and many mouse proteins will show activity on human cells. Other proteins may have a lower specific activity when used in the opposite species.

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The reconstitution calculator equation

Volume (to add to vial) = Mass (in vial) ÷ Desired Reconstitution Concentration

Volume (to add to vial) = Mass (in vial) ÷ Desired Reconstitution Concentration
= ÷

The dilution calculator equation

Concentration (start) × Volume (start) = Concentration (final) × Volume (final)

This equation is commonly abbreviated as: C1V1 = C2V2

浓度 (start) × 体积 (start) = 浓度 (final) × 体积 (final)
× = ×
C1   V1   C2   V2

The specific activity calculator equation

Specific Activity (Unit/mg) = 106 ÷ Biological Activity (ED50)

Specific Activity (Unit/mg) = 106 ÷ Biological Activity (ED50)
Unit/mg = 106 ÷ ng/mL

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产品名称:
FZD2 Protein, Mouse (HEK293, Fc)
目录号:
HY-P70918
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