HSF1 蛋白, Human (His)

HSF1 is a stress-inducible transcription factor that coordinates the heat shock response by binding to the heat shock element (HSE) and activating heat shock genes. In unstressed cells, it remains inactive in multichaperone complexes. HSF1 Protein, Human (His) is the recombinant human-derived HSF1 protein, expressed by E. coli , with N-6*His labeled tag.

HSF1 是一种应激反应性 DNA 结合转录因子，是热休克反应 (HSR) 的中心协调者，可导致多种热休克蛋白 (HSP) 的转录激活，这些热休克蛋白对于细胞保护免受损伤至关重要。在无应激条件下，HSF1 驻留在含有 HSP90 的多伴侣复合物中，保持失活的单体状态。当暴露于压力，特别是热时，HSF1 发生同源三聚化，并通过与启动子中的热休克元件 (HSE) 结合来激活 HSP 基因的转录。这种转变是可逆的，并且在 HSR 的恢复阶段，HSF1 返回到其未激活的形式。除了转录激活之外，HSF1 还参与各种细胞过程，包括与 TTC5/STRAP 和 p300/EP300 形成染色质相关复合物、抑制 Ras 诱导的 c-fos 基因转录、调节前 mRNA 加工、应激诱导的核输出mRNA 和有丝分裂进展的调节。此外，在微生物感染的情况下，HSF1 通过与 HIV-1 长末端重复启动子结合，在潜在人类免疫缺陷病毒 (HIV-1) 转录的重新激活中发挥作用。

Human

E. coli

N-6*His

Q00613-1 (D2-S529)

3297  [NCBI]

Partial

DLPVGPGAAGPSNVPAFLTKLWTLVSDPDTDALICWSPSGNSFHVFDQGQFAKEVLPKYFKHNNMASFVRQLNMYGFRKVVHIEQGGLVKPERDDTEFQHPCFLRGQEQLLENIKRKVTSVSTLKSEDIKIRQDSVTKLLTDVQLMKGKQECMDSKLLAMKHENEALWREVASLRQKHAQQQKVVNKLIQFLISLVQSNRILGVKRKIPLMLNDSGSAHSMPKYSRQFSLEHVHGSGPYSAPSPAYSSSSLYAPDAVASSGPIISDITELAPASPMASPGGSIDERPLSSSPLVRVKEEPPSPPQSPRVEEASPGRPSSVDTLLSPTALIDSILRESEPAPASVTALTDARGHTDTEGRPPSPPPTSTPEKCLSVACLDKNELSDHLDAMDSNLDNLQTMLSSHGFSVDTSALLDLFSPSVTVPDMSLPDLDSSLASIQELLSPQEPPRPPEAENSSPDSGKQLVHYTAQPLFLLDPGSVDTGSNDLPVLFELGEGSYFSEGDGFAEDPTISLLTGSEPPKAKDPTVS

Approximately 67 kDa

• 
  Greater than 90% as determined by reducing SDS-PAGE.

无菌粉末

Lyophilized a 0.22 μm filtered solution of PBS, pH 7.4 or PBS, pH 7.4, 8% trehalose.

<1 EU/μg, determined by LAL method.

It is not recommended to reconstitute to a concentration less than 100 μg/mL in ddH₂O. For long term storage it is recommended to add a carrier protein (0.1% BSA, 5% HSA, 10% FBS or 5% Trehalose).

Stored at -20°C for 2 years from date of receipt. After reconstitution, it is stable at 4°C for 1 week or -20°C for longer (with carrier protein). It is recommended to freeze aliquots at -20°C or -80°C for extended storage.

Room temperature in continental US; may vary elsewhere.