1. 重组蛋白
  2. Cytokines and Growth Factors
  3. Chemokine & Receptors
  4. CXC Chemokines
  5. I-TAC/CXCL11
  6. I-TAC/CXCL11 蛋白, Human (HEK293)

I-TAC/CXCL11 蛋白, Human (HEK293)

目录号: HY-P7228
COA 产品使用指南

CXCL11,也称为 IFN 诱导型 T 细胞 α-趋化因子 (I-TAC),属于 ELR 阴性 CXC 趋化因子家族。CXCL11 由多种细胞在干扰素 (IFN) 刺激后产生,包括白细胞、成纤维细胞和内皮细胞。CXCL11 通过结合CXCR3 发出信号。CXCL11 与多效性功能相关,包括趋化性迁移、调节细胞增殖和自我更新、增加细胞粘附和调节血管抑制作用。I-TAC/CXCL11 蛋白, Human (HEK293) 由 73 个氨基酸(F22-F94) 组成,在 HEK293 细胞中表达产生。

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规格 价格 是否有货 数量
10 μg ¥1600 In-stock
50 μg ¥3900 In-stock
100 μg   询价  
250 μg   询价  

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描述

CXCL11, also known as IFN-inducible T-cell α-chemoattractant (I-TAC), belongs to the ELR-negative CXC chemokine family. CXCL11 is produced by a variety of cells including leukocytes, fibroblasts, and endothelial cells upon stimulation with interferons (IFNs). CXCL11 signals through CXCR3. CXCL11 is associated with pleiotropic functions including chemotactic migration, regulation of cell proliferation and self-renewal, increasing cell adhesion, and modulation of angiostatic effects[1][2]. I-TAC/CXCL11 Protein, Human (HEK293) consists of 73 amino acids (F22-F94) and is expressed in HEK293 cells.

研究背景

CXCL11 is mainly expressed in the lung, pancreas, thymus, peripheral blood leukocytes, spleen, and liver and is expressed at lesser levels in the intestine, placenta, and prostate. CXCL11 is located on human chromosome 4 and is mainly secreted by cancer cells, leukocytes, monocytes, dendritic cells, endothelial cells, and fibroblasts. CXCL11 attracts activated lymphocytes and NK cells to inflamed tissue and to tumors and inhibits the generation of novel blood vessels essential for tumor growth and tissue repair[1][2].
CXCL11 which can bind to two different chemokine receptors, CXCR3 and CXCR7. CXCL11 is characterized by the presence of 1 amino acid in between the 2 NH2-terminal cysteines. CXCL11 is usually expressed at low levels in homeostatic conditions, but is upregulated during cancer or infectious disease processes. CXCL11 is mainly induced by IFN-γ and IFN-β and is weakly induced by IFN-α. CXCL11 has potent chemoattractant activity for IL-2 activated T cells and transfected cell lines expressing CXCR3, but not freshly isolated T cells, neutrophils or monocytes. Simultaneous stimulation of fibroblasts or endothelial cells with IFN-γ and interleukin-1β or the TLR3 ligand double-stranded RNA resulted in a synergistic increase of CXCL11 production. In leukocytes, bacterial LPS and PGN even inhibited interferon-induced CXCL11 production. CXCL11 attracts activated T-helper 1 (Th1) lymphocytes and natural killer (NK) cells. Furthermore, CXCL11 can bind to CXCR7, which is associated with invasiveness and reduces apoptosis of tumor cells[1][2].
The diverse functions of CXCL11 include inhibiting angiogenesis, affecting the proliferation of different cell types, playing a role in fibroblast directed carcinoma invasion, increasing adhesion properties, suppressing M2 macrophage polarization, and facilitating the migration of certain immune cells[1][2].

体外研究
(In Vitro)

Recombinant human CXCL11 (100 ng/mL) promotes proliferation and migration of IGROV-1 cells via CXCR3. Recombinant human CXCL11 (100 ng/mL; 5-60 min) increases the phosphorylated p44/42 and phosphorylated Akt in ovarian cancer cells[3].

生物活性

The ED50 is <0.5 μg/mL as measured by CHO-K1/Gα15/hCXCR3 cells (human Gα15 and human CXCR3 stably expressed in CHO-K1 cells).

种属

Human

表达系统

HEK293

标签

Tag Free

蛋白编号

O14625 (F22-F94)

基因 ID

6373  [NCBI]

中文名
重组人I-TAC/CXCL11 (HEK293表达)
同用名
rHuI-TAC/CXCL11; C-X-C motif chemokine 11; Beta-R1; H174; IP-9; SCYB11
氨基酸序列

FPMFKRGRCLCIGPGVKAVKVADIEKASIMYPSNNCDKIEVIITLKENKGQRCLNPKSKQARLIIKKVERKNF

分子量

Approximately 8.3 kDa

性状

Lyophilized powder.

组分

Lyophilized after extensive dialysis against PBS.

内毒素含量

<0.2 EU/μg, determined by LAL method.

复溶方法

It is not recommended to reconstitute to a concentration less than 100 μg/mL in ddH2O or PBS.

保存条件 & 期限

Stored at -20°C. After reconstitution, it is stable at 4°C for 1 week or -20°C for longer. It is recommended to freeze aliquots at -20°C or -80°C for extended storage.

运输条件

Room temperature in continental US; may vary elsewhere.

文件资料
参考文献

I-TAC/CXCL11 Protein, Human (HEK293) 相关分类

Help & FAQs
  • Do most proteins show cross-species activity?

    Species cross-reactivity must be investigated individually for each product. Many human cytokines will produce a nice response in mouse cell lines, and many mouse proteins will show activity on human cells. Other proteins may have a lower specific activity when used in the opposite species.

  • 复溶计算器

  • 重组蛋白稀释计算器

  • 比活力计算器

The reconstitution calculator equation

Volume (to add to vial) = Mass (in vial) ÷ Desired Reconstitution Concentration

Volume (to add to vial) = Mass (in vial) ÷ Desired Reconstitution Concentration
= ÷

The dilution calculator equation

Concentration (start) × Volume (start) = Concentration (final) × Volume (final)

This equation is commonly abbreviated as: C1V1 = C2V2

浓度 (start) × 体积 (start) = 浓度 (final) × 体积 (final)
× = ×
C1   V1   C2   V2

The specific active calculator equation

Specific Active (Unit/mg) = 106 ÷ Biological Activity (ED50)

Specific Active (Unit/mg) = 106 ÷ Biological Activity (ED50)
Unit/mg = 106 ÷ ng/mL

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产品名称:
I-TAC/CXCL11 Protein, Human (HEK293)
目录号:
HY-P7228
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