1. 重组蛋白
  2. Cytokines and Growth Factors
  3. Chemokine & Receptors
  4. CXC Chemokines
  5. I-TAC/CXCL11
  6. I-TAC/CXCL11 蛋白, Human (HEK293)

I-TAC/CXCL11 蛋白, Human (HEK293)

目录号: HY-P7228
COA 产品使用指南

CXCL11,也称为 IFN 诱导型 T 细胞 α-趋化因子 (I-TAC),属于 ELR 阴性 CXC 趋化因子家族。CXCL11 由多种细胞在干扰素 (IFN) 刺激后产生,包括白细胞、成纤维细胞和内皮细胞。CXCL11 通过结合CXCR3 发出信号。CXCL11 与多效性功能相关,包括趋化性迁移、调节细胞增殖和自我更新、增加细胞粘附和调节血管抑制作用。I-TAC/CXCL11 蛋白, Human (HEK293) 由 73 个氨基酸(F22-F94) 组成,在 HEK293 细胞中表达产生。

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规格 价格 是否有货 数量
10 μg ¥1600 In-stock
50 μg ¥3900 In-stock
100 μg   询价  

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描述

CXCL11, also known as IFN-inducible T-cell α-chemoattractant (I-TAC), belongs to the ELR-negative CXC chemokine family. CXCL11 is produced by a variety of cells including leukocytes, fibroblasts, and endothelial cells upon stimulation with interferons (IFNs). CXCL11 signals through CXCR3. CXCL11 is associated with pleiotropic functions including chemotactic migration, regulation of cell proliferation and self-renewal, increasing cell adhesion, and modulation of angiostatic effects[1][2]. I-TAC/CXCL11 Protein, Human (HEK293) consists of 73 amino acids (F22-F94) and is expressed in HEK293 cells.

研究背景

CXCL11主要在肺、胰腺、胸腺、外周血白细胞、脾脏和肝脏中表达,在肠道、胎盘和前列腺中表达量较低。CXCL11位于人类4号染色体上,主要由癌细胞、白细胞、单核细胞、树突状细胞、内皮细胞和成纤维细胞分泌。CXCL11 将活化的淋巴细胞和 NK 细胞吸引到发炎组织和肿瘤,并抑制肿瘤生长和组织修复所必需的新血管的生成[1][2]
CXCL11可以结合两种不同的趋化因子受体,CXCR3和CXCR7。CXCL11 的特征是 2 个 NH2 末端半胱氨酸之间存在 1 个氨基酸。CXCL11 在稳态条件下通常表达水平较低,但在癌症或传染病过程中表达上调。CXCL11主要受IFN-γ和IFN-β诱导,IFN-α诱导较弱。CXCL11 对 IL-2 激活的 T 细胞和表达 CXCR3 的转染细胞系具有有效的趋化活性,但对新鲜分离的 T 细胞、中性粒细胞或单核细胞没有作用。用 IFN-γ 和白介素-1β 或 TLR3 配体双链 RNA 同时刺激成纤维细胞或内皮细胞,导致 CXCL11 产量的协同增加。在白细胞中,细菌 LPS 和 PGN 甚至抑制干扰素诱导的 CXCL11 产生。CXCL11 吸引激活的辅助性 T 1 (Th1) 淋巴细胞和自然杀伤 (NK) 细胞。此外,CXCL11 可以与 CXCR7 结合,CXCR7 与侵袭性相关并减少肿瘤细胞的凋亡[1][2]
CXCL11 的多种功能包括抑制血管生成,影响不同细胞类型的增殖,在成纤维细胞定向癌侵袭中发挥作用,增加粘附特性,抑制 M2 巨噬细胞极化,并促进某些免疫细胞的迁移[1] [2]

体外研究
(In Vitro)

Recombinant human CXCL11 (100 ng/mL) promotes proliferation and migration of IGROV-1 cells via CXCR3. Recombinant human CXCL11 (100 ng/mL; 5-60 min) increases the phosphorylated p44/42 and phosphorylated Akt in ovarian cancer cells[3].

生物活性

1.The ED50 is <0.5 μg/mL as measured by CHO-K1/Gα15/hCXCR3 cells (human Gα15 and human CXCR3 stably expressed in CHO-K1 cells).
2.The biological activity determined by a chemotaxis bioassay using activated human T-lymphocytes. The ED50 for this effect is 2.251 ng/mL in the presence of 1ng/mL human IL-2, corresponding to a specific activity is 4.442×105 U/mg.

种属

Human

表达系统

HEK293

标签

Tag Free

蛋白编号

O14625 (F22-F94)

基因 ID
蛋白结构
N-term
CXCL11 (F22-F94)
Accession # O14625
C-term
中文名
重组人I-TAC/CXCL11 (HEK293表达)
同用名
rHuI-TAC/CXCL11; C-X-C motif chemokine 11; Beta-R1; H174; IP-9; SCYB11
氨基酸序列

FPMFKRGRCLCIGPGVKAVKVADIEKASIMYPSNNCDKIEVIITLKENKGQRCLNPKSKQARLIIKKVERKNF

分子量

Approximately 8.3 kDa

纯度

Greater than 95% as determined by reducing SDS-PAGE.

性状

无菌粉末.

组分

Lyophilized after extensive dialysis against PBS.

内毒素含量

<0.2 EU/μg, determined by LAL method.

复溶方法

It is not recommended to reconstitute to a concentration less than 100 μg/mL in ddH2O. For long term storage it is recommended to add a carrier protein (0.1% BSA, 5% HSA, 10% FBS or 5% Trehalose).

保存条件 & 期限

Stored at -20°C for 2 years. After reconstitution, it is stable at 4°C for 1 week or -20°C for longer (with carrier protein). It is recommended to freeze aliquots at -20°C or -80°C for extended storage.

运输条件

Room temperature in continental US; may vary elsewhere.

文件资料
参考文献

I-TAC/CXCL11 Protein, Human (HEK293) 相关分类

Help & FAQs
  • Do most proteins show cross-species activity?

    Species cross-reactivity must be investigated individually for each product. Many human cytokines will produce a nice response in mouse cell lines, and many mouse proteins will show activity on human cells. Other proteins may have a lower specific activity when used in the opposite species.

  • 复溶计算器

  • 重组蛋白稀释计算器

  • 比活力计算器

The reconstitution calculator equation

Volume (to add to vial) = Mass (in vial) ÷ Desired Reconstitution Concentration

Volume (to add to vial) = Mass (in vial) ÷ Desired Reconstitution Concentration
= ÷

The dilution calculator equation

Concentration (start) × Volume (start) = Concentration (final) × Volume (final)

This equation is commonly abbreviated as: C1V1 = C2V2

浓度 (start) × 体积 (start) = 浓度 (final) × 体积 (final)
× = ×
C1   V1   C2   V2

The specific activity calculator equation

Specific Activity (Unit/mg) = 106 ÷ Biological Activity (ED50)

Specific Activity (Unit/mg) = 106 ÷ Biological Activity (ED50)
Unit/mg = 106 ÷ ng/mL

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I-TAC/CXCL11 Protein, Human (HEK293)
目录号:
HY-P7228
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