IA2 蛋白, Human (293a.a, N-His)

IA2 Protein is pivotal in vesicle-mediated secretory processes, crucial for secretory vesicle accumulation in the hippocampus, pituitary, and pancreatic islets. Essential for insulin-related functions, IA2 ensures normal insulin-containing vesicle levels, preventing degradation and promoting glucose-stimulated insulin secretion. IA2's influence extends to neurotransmitter regulation, required for normal norepinephrine, dopamine, and serotonin accumulation. In females, IA2 is uniquely essential for pituitary hormone accumulation and secretion. It also maintains normal renin expression. Despite lacking intrinsic enzyme activity, IA2 may regulate active protein-tyrosine phosphatases, like PTPRA, through dimerization. IA2's interaction with ICA512-TMF highlights its role in modulating insulin secretory granule dynamics and proposes a tethering mechanism to the actin cytoskeleton, dependent on UTRN and regulated by ICA512-CFF dimerization. IA2 Protein, Human (293a.a, N-His) is the recombinant human-derived IA2 protein, expressed by E. coli , with N-6*His labeled tag. The total length of IA2 Protein, Human (293a.a, N-His) is 293 a.a., with molecular weight of ~35 kDa.

IA2 蛋白在囊泡介导的分泌过程中发挥着关键作用，其基本功能遍及各种组织。它是海马体、垂体和胰岛等关键区域分泌囊泡正常积累所必需的。 IA2 对于胰岛素相关过程尤其重要，它对于正常水平的含胰岛素囊泡的积累、防止其降解并有助于响应葡萄糖刺激而分泌胰岛素是必需的。 IA2 的影响还延伸到大脑中的神经递质调节，这是去甲肾上腺素、多巴胺和血清素正常积累所必需的。有趣的是，在女性中，IA2 对于黄体生成素 (LH) 和卵泡刺激素 (FSH) 等垂体激素的正常积累和分泌是不可或缺的。此外，IA2 在维持肾素表达和释放的正常水平方面发挥作用。尽管缺乏内在酶活性，IA2 可以通过二聚化调节催化活性蛋白酪氨酸磷酸酶，例如 PTPRA。 IA2 复杂的动力学在其与 ICA512-TMF 的相互作用中得到进一步强调，它调节胰岛素分泌颗粒的动力学和胞吐作用，提出了依赖于 UTRN 并受 ICA512-CFF 二聚化调节的肌动蛋白细胞骨架的束缚机制。

Data is not available.

Human

E. coli

N-6*His

Q16849-1 (M687-Q979)

5798  [NCBI]

MDISTGHMILAYMEDHLRNRDRLAKEWQALCAYQAEPNTCATAQGEGNIKKNRHPDFLPYDHARIKLKVESSPSRSDYINASPIIEHDPRMPAYIATQGPLSHTIADFWQMVWESGCTVIVMLTPLVEDGVKQCDRYWPDEGASLYHVYEVNLVSEHIWCEDFLVRSFYLKNVQTQETRTLTQFHFLSWPAEGTPASTRPLLDFRRKVNKCYRGRSCPIIVHCSDGAGRTGTYILIDMVLNRMAKGVKEIDIAATLEHVRDQRPGLVRSKDQFEFALTAVAEEVNAILKALPQ

Approximately 35 kDa

• 
  Greater than 95% as determined by reducing SDS-PAGE.

无菌粉末.

Lyophilized from a 0.2 μm filtered solution of 50 mM Tris-HCL, 300 mM NaCl, pH 7.4.

<1 EU/μg, determined by LAL method.

It is not recommended to reconstitute to a concentration less than 100 μg/mL in ddH₂O. For long term storage it is recommended to add a carrier protein (0.1% BSA, 5% HSA, 10% FBS or 5% Trehalose).

Stored at -20°C for 2 years. After reconstitution, it is stable at 4°C for 1 week or -20°C for longer (with carrier protein). It is recommended to freeze aliquots at -20°C or -80°C for extended storage.

Room temperature in continental US; may vary elsewhere.