IFN-alpha/beta R2 蛋白, Mouse (HEK293, His)

IFN-α/β R2 protein and IFNAR1 together constitute the heterodimeric receptor of type I interferon and initiate the JAK-STAT signaling cascade. Upon binding of type I interferons, IFNAR1 and IFNAR2 activate related Janus kinases (TYK2 and JAK1) in proximity, resulting in cross-phosphorylation. IFN-alpha/beta R2 Protein, Mouse (HEK293, His) is the recombinant mouse-derived IFN-alpha/beta R2 protein, expressed by HEK293 , with C-His, C-6*His labeled tag. The total length of IFN-alpha/beta R2 Protein, Mouse (HEK293, His) is 221 a.a., with molecular weight of ~37-57 kDa.

IFN-α/β R2 蛋白与 IFNAR1 一起构成 I 型干扰素的异二聚体受体，包括干扰素 α、β、ε、Ω 和 κ。I 型干扰素结合后，受体激活启动 JAK-STAT 信号级联，导致介导干扰素反应的干扰素调节基因的转录激活或抑制。从机制上讲，I 型干扰素的结合使 IFNAR1 和 IFNAR2 非常接近，促进其相关 Janus 激酶 (JAK) 的交叉磷酸化，其中 TYK2 与 IFNAR1 结合，JAK1 与 IFNAR2 结合。然后，激活的 JAK 磷酸化 IFNAR1 和 IFNAR2 胞内结构域上的特定酪氨酸残基，为 STAT 转录因子（STAT1、STAT2 和 STAT）创建对接位点。这些磷酸化的 STAT 蛋白易位到细胞核中，调节干扰素调节基因的表达。此外，IFN-α/β R2 蛋白可作为 I 型干扰素受体活性的有效抑制剂，展示其在调节干扰素信号通路中的作用。

Immobilized Mouse IFNA2 at 10 μg/mL (100 μL/well) can bind IFNAR2. The ED50 for this effect is 126.3 ng/mL.

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  Immobilized Mouse IFNA2 at 10 μg/mL (100 μL/well) can bind IFNAR2 , The ED₅₀ for this effect is 126.3 ng/mL.

Mouse

HEK293

C-His;C-6*His

O35664-1 (S22-A242)

15976  [NCBI]

SLETITPSAFDGYPDEPCTINITIRNSRLILSWELENKSGPPANYTLWYTVMSKDENLTKVKNCSDTTKSSCDVTDKWLEGMESYVVAIVIVHRGDLTVCRCSDYIVPANAPLEPPEFEIVGFTDHINVTMEFPPVTSKIIQEKMKTTPFVIKEQIGDSVRKKHEPKVNNVTGNFTFVLRDLLPKTNYCVSLYFDDDPAIKSPLKCIVLQPGQESGLSESA

Approximately 37-57 kDa

Greater than 95% as determined by reducing SDS-PAGE.

无菌粉末.

Lyophilized a 0.22 μm filtered solution of PBS, pH 7.4.

<1 EU/μg, determined by LAL method.

It is not recommended to reconstitute to a concentration less than 100 μg/mL in ddH₂O. For long term storage it is recommended to add a carrier protein (0.1% BSA, 5% HSA, 10% FBS or 5% Trehalose).

Stored at -20°C for 2 years. After reconstitution, it is stable at 4°C for 1 week or -20°C for longer (with carrier protein). It is recommended to freeze aliquots at -20°C or -80°C for extended storage.

Room temperature in continental US; may vary elsewhere.