1. 重组蛋白
  2. Cytokines and Growth Factors
  3. Interleukin & Receptors
  4. IL-27 Receptor
  5. IL27RA
  6. IL-27R alpha/TCCR 蛋白, Human (514a.a, HEK293, Fc)

IL-27R alpha/TCCR 蛋白, Human (514a.a, HEK293, Fc)

目录号: HY-P77223
产品使用指南

IL-27R α (白细胞介素 27 受体 α) 是白细胞介素 27 受体的一个亚基,IL-27 受体是一种具有广泛免疫功能的炎性细胞因子。IL-27R α 可用作天然的 IL-27 拮抗剂。IL-27R alpha/TCCR 蛋白, Human (514a.a, HEK293, Fc) 是一种重组蛋白,C 末端带有 Fc 标签,由 514 个氨基酸组成 (M1-R514),在 HEK293 细胞中表达。

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100 μg 询问价格 & 货期

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描述

IL-27R alpha(Interleukin 27 receptor alpha) is a subunit of the interleukin-27 receptor, an inflammatory cytokine that possesses extensive immunological functions. IL-27R alphacan be used as a natural IL-27 antagonist[1]. IL-27R alpha/TCCR Protein, Human (514a.a, HEK293, Fc) is a recombinant protein with a Fc label that consists of 514 amino acids (M1-R514) and is produced by HEK293 cells.

研究背景

IL-27Rα is specific for IL-27 and is primarily expressed on the cell surface of inflammatory cells, including T, B, and NK lymphocytes, neutrophils, monocytes, and mast cells, although it is found at lower levels in macrophages, hepatocytes, keratinocytes, and endothelial cells[2].
The amino acid sequence of human IL-27R alpha protein has low homology for mouse IL-27R alpha protein.
IL-27Rα (WSX-1) and gp130 compose the IL-27 receptor-signaling complex. Binding of IL-27 to its heterodimeric receptor complex leads to the intracellular recruitment and activation of the Janus kinase (JAK)/STAT-1 and STAT-3 pathways, suppress the expression of inflammatory cytokines[3].
IL-27R alpha is a class I cytokine receptor with homology to gp130 of IL-6R and to IL-12R families. IL-27R alpha can be used as a natural IL-27 antagonist[1]. IL-27R alpha can be used as an independent prognostic aGVHD biomarker[4]. IL-27/IL-27R alpha also plays an important role in the down-regulation of airway hyper-reactivity and lung inflammation during the development of allergic asthma through its suppressive effect on cytokine production[5]. IL-27/IL-27RA signaling can suppress the expression of inflammatory cytokines, IL-6 and IL-8, induced by LPS stimulation, leading to phosphorylation of STAT1 and STAT3[6].

生物活性

Measured by its binding ability in a functional ELISA. Immobilized human IL27 at 20 μg/mL (100 μL/well) can bind human IL27Ra with a linear range of 0.064-1.6 μg/mL.

种属

Others

表达系统

HEK293

标签

C-hFc

蛋白编号

Q6UWB1 (M1-R514)

基因 ID

9466  [NCBI]

中文名
HY-P77223重组人白介素IL-27 受体 α蛋白
同用名
Interleukin-27 receptor subunit alpha; IL-27R-alpha; TCCR; WSX-1; IL-27RA
分子量

105-115 kDa

纯度

Greater than 95% as determined by reducing SDS-PAGE

性状

Lyophilized powder.

组分

Lyophilized a 0.2 μm filtered solution of PBS, pH 7.4, 8% trehalose, 8% mannitol

内毒素含量

<1 EU/μg, determined by LAL method.

复溶方法

It is not recommended to reconstitute to a concentration less than 100 μg/mL in ddH2O. For long term storage it is recommended to add a carrier protein (0.1% BSA, 5% HSA, 10% FBS or 5% Trehalose).

保存条件 & 期限

Stored at -20°C. After reconstitution, it is stable at 4°C for 1 week or -20°C for longer. It is recommended to freeze aliquots at -20°C or -80°C for extended storage.

运输条件

Room temperature in continental US; may vary elsewhere.

文件资料
参考文献

IL-27R alpha/TCCR Protein, Human (514a.a, HEK293, Fc) 相关分类

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The reconstitution calculator equation

Volume (to add to vial) = Mass (in vial) ÷ Desired Reconstitution Concentration

Volume (to add to vial) = Mass (in vial) ÷ Desired Reconstitution Concentration
= ÷

The dilution calculator equation

Concentration (start) × Volume (start) = Concentration (final) × Volume (final)

This equation is commonly abbreviated as: C1V1 = C2V2

浓度 (start) × 体积 (start) = 浓度 (final) × 体积 (final)
× = ×
C1   V1   C2   V2

The specific active calculator equation

Specific Active (Unit/mg) = 106 ÷ Biological Activity (ED50)

Specific Active (Unit/mg) = 106 ÷ Biological Activity (ED50)
Unit/mg = 106 ÷ ng/mL

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IL-27R alpha/TCCR Protein, Human (514a.a, HEK293, Fc)
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HY-P77223
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