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  3. MBIP 蛋白, Human (His)

MBIP 蛋白, Human (His)

目录号: HY-P70921
产品使用指南

MBIP 蛋白抑制 MAP3K12 活性并阻止 JNK/SAPK 通路的激活。它是 ATAC 复合体的重要组成部分,ATAC 复合体是一种针对组蛋白 H3 和 H4 的组蛋白乙酰转移酶复合体。MBIP 蛋白, Human (His) 是重组的 MBIP 蛋白,由 E. coli 表达,带有 N-6*His 标签。MBIP 蛋白, Human (His) 全长 344 个氨基酸,分子量约为 ~50.0 kDa。

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描述

MBIP protein inhibits MAP3K12 activity and prevents activation of the JNK/SAPK pathway. It is an important component of the ATAC complex, a histone acetyltransferase complex targeting histones H3 and H4. MBIP Protein, Human (His) is the recombinant human-derived MBIP protein, expressed by E. coli , with N-6*His labeled tag. The total length of MBIP Protein, Human (His) is 344 a.a., with molecular weight of ~50.0 kDa.

研究背景

MBIP蛋白通过抑制MAP3K12的活性发挥抑制作用,从而阻止JNK/SAPK通路的激活。此外,MBIP 是 ATAC 复合体的重要组成部分,ATAC 是一种对组蛋白 H3 和 H4 具有活性的组蛋白乙酰转移酶复合体。在包含 ADA2A 的复合物 (ATAC) 中,MBIP 与其他组件协作,包括 KAT14、KAT2A、TADA2L、TADA3L、ZZ3、WDR5、YEATS2、CCDC101 和 DR1。在这个复杂的复合体中,MBIP 很可能与 KAT2A、KAT14 和 WDR5 直接相互作用,突出了它在染色质修饰过程中的不可或缺的作用。

种属

Human

表达系统

E. coli

标签

N-6*His

蛋白编号

Q9NS73 (M1-P344)

基因 ID
蛋白结构
N-term
6*His
MBIP (M1-P344)
Accession # Q9NS73
C-term
同用名
MAP3K12-Binding Inhibitory Protein 1; MAPK Upstream Kinase-Binding Inhibitory Protein; MUK-Binding Inhibitory Protein; MBIP
氨基酸序列

MAAATELNRPSSGDRNLERRCRPNLSREVLYEIFRSLHTLVGQLDLRDDVVKITIDWNKLQSLSAFQPALLFSALEQHILYLQPFLAKLQSPIKEENTTAVEEIGRTEMGNKNEVNDKFSIGDLQEEEKHKESDLRDVKKTQIHFDPEVVQIKAGKAEIDRRISAFIERKQAEINENNVREFCNVIDCNQENSCARTDAIFTPYPGFKSHVKVSRVVNTYGPQTRPEGIPGSGHKPNSMLRDCGNQAVEERLQNIEAHLRLQTGGPVPRDIYQRIKKLEDKILELEGISPEYFQSVSFSGKRRKVQPPQQNYSLAELDEKISALKQALLRKSREAESMATHHLP

分子量

Approximately 50.0 kDa

纯度

Greater than 95% as determined by reducing SDS-PAGE.

内毒素含量

<1 EU/μg, determined by LAL method.

文件资料

MBIP Protein, Human (His) 相关分类

Help & FAQs
  • Do most proteins show cross-species activity?

    Species cross-reactivity must be investigated individually for each product. Many human cytokines will produce a nice response in mouse cell lines, and many mouse proteins will show activity on human cells. Other proteins may have a lower specific activity when used in the opposite species.

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The reconstitution calculator equation

Volume (to add to vial) = Mass (in vial) ÷ Desired Reconstitution Concentration

Volume (to add to vial) = Mass (in vial) ÷ Desired Reconstitution Concentration
= ÷

The dilution calculator equation

Concentration (start) × Volume (start) = Concentration (final) × Volume (final)

This equation is commonly abbreviated as: C1V1 = C2V2

浓度 (start) × 体积 (start) = 浓度 (final) × 体积 (final)
× = ×
C1   V1   C2   V2

The specific activity calculator equation

Specific Activity (Unit/mg) = 106 ÷ Biological Activity (ED50)

Specific Activity (Unit/mg) = 106 ÷ Biological Activity (ED50)
Unit/mg = 106 ÷ ng/mL

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产品名称:
MBIP Protein, Human (His)
目录号:
HY-P70921
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