1. 重组蛋白
  2. Cytokines and Growth Factors
  3. Chemokine & Receptors
  4. CC Chemokines
  5. MIP-1 beta/CCL4
  6. CCL4 蛋白, Human

CCL4 蛋白, Human

目录号: HY-P7257
COA 产品使用指南

CCL4 蛋白, Human 是一种 CC 趋化因子亚家族的小细胞因子,可以与细胞表面的 CCR5 趋化因子受体结合作用。能够在各种炎症条件下促进白细胞聚集,又有助于对人类免疫缺陷 1 型病毒产生免疫保护。CCL4 蛋白, Human 是由 E. coli 表达的重组人 CCL4 (A24-N92)。

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10 μg ¥750 In-stock
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100 μg ¥3500 In-stock
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  • 生物活性

  • 技术参数

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  • 产品资料

  • 参考文献

描述

CCL4 Protein, Human is a small cytokine of the CC chemokine subfamily that binds to the CCR5 chemokine receptor on the cell surface, promotes leukocyte aggregation under various inflammatory conditions, and contributes to immune protection against human immunodeficiency virus type 1. CCL4 Protein, Human is a recombinant human CCL4 (A24-N92) expressed by E. coli[1].

研究背景

CCL4又名巨噬细胞炎症蛋白(MIP-1β),属于CC趋化因子家族,是人体中由CCL4基因编码的蛋白质,属于17号染色体17q11-q21区域,分子量约为8-10 kDa。CCL4 可由单核细胞、B 细胞、T 细胞、NK 细胞、树突状细胞、中性粒细胞、成纤维细胞、内皮细胞和上皮细胞产生[1]。CCL4 充当趋化因子,与 G 蛋白偶联受体 CCR5CCR8 结合,并充当炎症或受损组织部位的自然杀伤细胞、单核细胞和各种其他免疫细胞的趋化剂。与此同时,CCL4 还可以作为 CD8+ T 细胞产生的主要 HIV 抑制因子,诱导不同毒株的 HIV-1、HIV-2 和猴免疫缺陷病毒 (SIV) 的剂量依赖性抑制。其中,N端加工形式CCL4(3-69)是由外周血淋巴细胞分泌后蛋白水解裂解产生的,保留了诱导趋化因子受体CCR5表面表达下调并抑制CCR5介导的HIV-1进入的能力进入 T 细胞[2]

生物活性

1. The ED50 is <100 ng/mL as measured by CHO-K1/Gα15/hCCR5 cells (human Gα15 and human CCR5 stably expressed in CHO-K1 cells).
2. Measured by its ability to chemoattract THP-1 human acute monocytic leukemia cells. The ED50 this effect is 2.785 ng/mL, corresponding to a specific activity is 3.59×105 U/mg.

  • Measured by its ability to chemoattract THP-1 human acute monocytic leukemia cells. The ED50 for this effect is 2.785 ng/mL, corresponding to a specific activity is 3.59×105 U/mg.
种属

Human

表达系统

E. coli

标签

Tag Free

蛋白编号

P13236 (A24-N92)

基因 ID
蛋白结构
N-term
CCL4 (A24-N92)
Accession # P13236
C-term
中文名
重组人 MIP-1 beta/CCL4
同用名
rHuMIP-1β/CCL4; C-C motif chemokine 4; LAG-1; Macrophage inflammatory protein 1-beta; SCYA4
氨基酸序列

APMGSDPPTACCFSYTARKLPRNFVVDYYETSSLCSQPAVVFQTKRSKQVCADPSESWVQEYVYDLELN

分子量

7-17 kDa

纯度
  • Greater than 95% as determined by reducing SDS-PAGE.
性状

无菌粉末.

组分

Lyophilized from a 0.2 μm filtered solution of PBS or 20 mM PB, 150 mM NaCl, pH 7.4.

内毒素含量

<1 EU/μg, determined by LAL method.

复溶方法

It is not recommended to reconstitute to a concentration less than 100 μg/mL in ddH2O. For long term storage it is recommended to add a carrier protein (0.1% BSA, 5% HSA, 10% FBS or 5% Trehalose).

保存条件 & 期限

Stored at -20°C for 2 years. After reconstitution, it is stable at 4°C for 1 week or -20°C for longer (with carrier protein). It is recommended to freeze aliquots at -20°C or -80°C for extended storage.

运输条件

Room temperature in continental US; may vary elsewhere.

文件资料
参考文献
Help & FAQs
  • Do most proteins show cross-species activity?

    Species cross-reactivity must be investigated individually for each product. Many human cytokines will produce a nice response in mouse cell lines, and many mouse proteins will show activity on human cells. Other proteins may have a lower specific activity when used in the opposite species.

  • 复溶计算器

  • 重组蛋白稀释计算器

  • 比活力计算器

The reconstitution calculator equation

Volume (to add to vial) = Mass (in vial) ÷ Desired Reconstitution Concentration

Volume (to add to vial) = Mass (in vial) ÷ Desired Reconstitution Concentration
= ÷

The dilution calculator equation

Concentration (start) × Volume (start) = Concentration (final) × Volume (final)

This equation is commonly abbreviated as: C1V1 = C2V2

浓度 (start) × 体积 (start) = 浓度 (final) × 体积 (final)
× = ×
C1   V1   C2   V2

The specific activity calculator equation

Specific Activity (Unit/mg) = 106 ÷ Biological Activity (ED50)

Specific Activity (Unit/mg) = 106 ÷ Biological Activity (ED50)
Unit/mg = 106 ÷ ng/mL

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产品名称:
CCL4 Protein, Human
目录号:
HY-P7257
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