MUSK 蛋白, Mouse (Myc, His-SUMO)

MUSK protein is a receptor tyrosine kinase that plays a critical role in the formation of the neuromuscular junction (NMJ). MUSK is activated by the AGRIN-LRP4 complex and regulates the NMJ through gene expression, actin cytoskeleton reorganization, and acetylcholine receptor (AChR) clustering. MUSK Protein, Mouse (Myc, His-SUMO) is the recombinant mouse-derived MUSK protein, expressed by E. coli , with C-Myc, N-SUMO, N-10*His labeled tag. The total length of MUSK Protein, Mouse (Myc, His-SUMO) is 473 a.a., with molecular weight of ~69.3 kDa.

MUSK 蛋白是一种受体酪氨酸激酶，在神经肌肉接头 (NMJ)（连接运动神经元和骨骼肌的突触）的形成和维护中发挥着至关重要的作用。LRP4 通过招募 AGRIN 来激活 MUSK 信号复合物，从而导致 MUSK 的磷酸化和激活。在肌管中，MUSK 的激活通过控制多个过程来调节 NMJ 的形成，包括突触下核中特定基因的表达、肌动蛋白细胞骨架的重组以及突触后膜中乙酰胆碱受体 (AChR) 的聚集。AChR 磷酸化和聚类的这种调节是由 ABL1 和 Src 家族激酶的激活介导的，而 ABL1 和 Src 家族激酶又反过来调节 MUSK。此外，MUSK 与 DVL1 和 PAK1 形成三元复合物，这对于 MUSK 依赖性的 AChR 聚类调节非常重要。MUSK 还可以通过 FNTA 正向调节 Rho 家族 GTPases，介导 RAC1（肌动蛋白细胞骨架和基因表达的关键调节因子）的磷酸化和激活。MUSK 信号传导的下游效应器包括 DNAJA3，其在 MUSK 下游发挥作用。此外，麝香还可能通过介导胆碱能反应、突触可塑性和记忆形成在中枢神经系统中发挥作用。

The enzyme activity of this recombinant protein is testing in progress, we cannot offer a guarantee yet.

Mouse

E. coli

C-Myc;N-SUMO;N-10*His

Q61006 (E22-S494)

18198  [NCBI]

EKLPKAPVITTPLETVDALVEEVATFMCAVESYPQPEISWTRNKILIKLFDTRYSIRENGQLLTILSVEDSDDGIYCCIANNGVGGAVESCGALQVKMKPKITRPPINVKIIEGLKAVLPCTTMGNPKPSVSWIKGDNALRENSRIAVLESGSLRIHNVQKEDAGQYRCVAKNSLGTAYSKLVKLEVEVFARILRAPESHNVTFGSFVTLRCTAIGIPVPTISWIENGNAVSSGSIQESVKDRVIDSRLQLFITKPGLYTCIATNKHGEKFSTAKAAATVSIAEWSKSQKDSQGYCAQYRGEVCDAVLAKDALVFFNTSYRDPEDAQELLIHTAWNELKAVSPLCRPAAEALLCNHLFQECSPGVVPTPMPICREYCLAVKELFCAKEWQAMEGKAHRGLYRSGMHLLPVPECSKLPSMHRDPTACTRLPYLDYKKENITTFPSITS

Approximately 69.3 kDa

Greater than 90% as determined by reducing SDS-PAGE.

无菌粉末.

Lyophilized after extensive dialysis against solution in 10 mM Tris-HCl, 1 mM EDTA, 6% Trehalose, pH 8.0.

<1 EU/μg, determined by LAL method.

It is not recommended to reconstitute to a concentration less than 100 μg/mL in ddH₂O.

Stored at -20°C for 2 years. After reconstitution, it is stable at 4°C for 1 week or -20°C for longer (with carrier protein). It is recommended to freeze aliquots at -20°C or -80°C for extended storage.

Room temperature in continental US; may vary elsewhere.