1. 重组蛋白
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  3. Norrin 蛋白, Human

Norrin 蛋白通过与 FZD4 和 LRP5 结合激活经典 Wnt 信号传导,促进视网膜血管化并稳定 β-连环蛋白 (CTNNB1)。它与 TSPAN12 合作独立激活 FZD4,这表明了一种不依赖于 Wnt 的机制。Norrin 蛋白, Human 是重组的 诺里蛋白,由 E. coli 表达,不带标签。

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5 μg ¥1350 In-stock
10 μg ¥2150 In-stock
50 μg ¥6000 In-stock
100 μg ¥9600 In-stock
500 μg ¥26800 In-stock
1 mg ¥42800 In-stock
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  • 生物活性

  • 技术参数

  • 产品性质

  • 产品资料

描述

Norrin protein activates canonical Wnt signaling by binding to FZD4 and LRP5, promotes retinal vascularization and stabilizes β-catenin (CTNNB1). It cooperates with TSPAN12 to independently activate FZD4, suggesting a Wnt-independent mechanism. Norrin Protein, Human is the recombinant human-derived Norrin protein, expressed by E. coli , with tag free.

研究背景

Norrin 蛋白作为经典 Wnt 信号通路中的重要参与者,通过与 FZD4 和 LRP5 辅助受体复合物结合来激活该通路。它的重要性在于其在视网膜血管化中的核心作用,因为它作为 FZD4 的配体,导致 β-连环蛋白 (CTNNB1) 的稳定和 LEF/TCF 介导的转录程序的激活。此外,Norrin 蛋白与 TSPAN12 合作独立激活 FZD4,表明存在不依赖于 Wnt 的信号传导机制,该机制也有助于 β-连环蛋白 (CTNNB1) 的积累。此外,诺里蛋白可能参与调节神经细胞分化和增殖的途径,并且它可能在神经外胚层细胞间相互作用中发挥作用。它通过二硫键形成同二聚体,并且是由 TSPAN12、FZD4、LRP5/6 和去甲蛋白本身 (NDP) 组成的复合物的一部分。值得注意的是,Norrin 蛋白表现出与 FZD4 结合的高亲和力,并与 LRP6 相互作用,特别是与其 Beta 螺旋桨 1 和 2 相互作用。

生物活性

Measured by its binding ability in a functional ELISA. When Human Norrin is coated at 0.5 μg/mL (100 μL/well) can bind Human Frizzled­4. The ED50 for this effect is 86.34 ng/mL.

  • Measured by its binding ability in a functional ELISA. When Human Norrin is coated at 0.5 μg/mL (100 μL/well) can bind Human Frizzled­4. The ED50 for this effect is 86.34 ng/mL.
种属

Human

表达系统

E. coli

标签

Tag Free

蛋白编号

Q00604 (K25-S133)

基因 ID
蛋白结构
N-term
Norrin (K25-S133)
Accession # Q00604
C-term
蛋白长度

Full Length of Mature Protein

中文名
Norrin 蛋白
同用名
Norrin; NDP; Norrie disease protein; X-linked exudative vitreoretinopathy 2 protein; EVR2
氨基酸序列

KTDSSFIMDSDPRRCMRHHYVDSISHPLYKCSSKMVLLARCEGHCSQASRSEPLVSFSTVLKQPFRSSCHCCRPQTSKLKALRLRCSGGMRLTATYRYILSCHCEECNS

分子量

Approximately 13 kDa

纯度
  • Greater than 95% as determined by reducing SDS-PAGE.
性状

无菌粉末.

组分

Lyophilized from a 0.22 μm filtered solution of 4 mM HCl, pH 2.5.

内毒素含量

<1 EU/μg, determined by LAL method.

复溶方法

It is not recommended to reconstitute to a concentration less than 100 μg/mL in ddH2O. For long term storage it is recommended to add a carrier protein (0.1% BSA, 5% HSA, 10% FBS or 5% Trehalose).

保存条件 & 期限

Stored at -20°C for 2 years from date of receipt. After reconstitution, it is stable at 4°C for 1 week or -20°C for longer (with carrier protein). It is recommended to freeze aliquots at -20°C or -80°C for extended storage.

运输条件

Room temperature in continental US; may vary elsewhere.

文件资料

Norrin Protein, Human 相关分类

Help & FAQs
  • Do most proteins show cross-species activity?

    Species cross-reactivity must be investigated individually for each product. Many human cytokines will produce a nice response in mouse cell lines, and many mouse proteins will show activity on human cells. Other proteins may have a lower specific activity when used in the opposite species.

  • 复溶计算器

  • 重组蛋白稀释计算器

  • 比活力计算器

The reconstitution calculator equation

Volume (to add to vial) = Mass (in vial) ÷ Desired Reconstitution Concentration

Volume (to add to vial) = Mass (in vial) ÷ Desired Reconstitution Concentration
= ÷

The dilution calculator equation

Concentration (start) × Volume (start) = Concentration (final) × Volume (final)

This equation is commonly abbreviated as: C1V1 = C2V2

浓度 (start) × 体积 (start) = 浓度 (final) × 体积 (final)
× = ×
C1   V1   C2   V2

The specific activity calculator equation

Specific Activity (Unit/mg) = 106 ÷ Biological Activity (ED50)

Specific Activity (Unit/mg) = 106 ÷ Biological Activity (ED50)
Unit/mg = 106 ÷ ng/mL

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产品名称:
Norrin Protein, Human
目录号:
HY-P79124
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