PLA2G1B 蛋白, Mouse (HEK293, His)

The PLA2G1B protein is a secreted calcium-dependent phospholipase A2 that targets dietary phospholipids in the intestine.Phosphatidylethanolamine and phosphatidylglycerol are preferred, which hydrolyze the ester bond of the fatty acyl group at the sn-2 position.PLA2G1B Protein, Mouse (HEK293, His) is the recombinant mouse-derived PLA2G1B protein, expressed by HEK293 , with C-6*His labeled tag.

PLA2G1B 是一种分泌型钙依赖性磷脂酶 A2，主要针对肠道中的膳食磷脂。它具有磷脂酶 A2 活性，可水解磷脂 sn-2 位脂肪酰基的酯键，优先水解磷脂酰乙醇胺和磷脂酰甘油而不是磷脂酰胆碱。PLA2G1B 可能在 N-酰基乙醇胺的生物合成中发挥至关重要的作用，调节肠道能量代谢和炎症。它以自分泌和旁分泌方式发挥作用，与 PLA2R1 受体结合后，可以调节足细胞存活和肾小球稳态。此外，PLA2G1B 具有抗蠕虫活性，在肠道上皮感染（肠道微生物群调节的过程）过程中直接影响蠕虫幼虫膜中磷脂酰乙醇胺的含量，从而影响蠕虫幼虫的完整性和传染性。

The enzyme activity of this recombinant protein is testing in progress, we cannot offer a guarantee yet.

Materials
Assay buffer: 50 mM HEPES, 1 mg/mL BSA, pH 7.0
Substrate buffer: 50 mM HEPES, 20 mM CaCl?, 1 mg/mL BSA, pH 7.0
PLA2G1B Protein, Mouse (HEK293, His) (HY-P71212)
Trypsin
Substrate: 1-hexadecanoyl-2-(1-pyrenedecanoyl)-sn-glycero-3-phosphocholine (16:0-Pyr PC). Dilute to 2 mM in DMSO first, then dilute to a final stock concentration of 400 μM in ethanol
Standard: 1-Pyrenedecanoic Acid

Procedure
1. Preparation of standard curve: Dilute the standard with assay buffer to concentrations of 0, 0.1, 1, 2.5, 5, 10, 25, 50, and 100 μM. Add 100 μL of each dilution to a 96-well black microplate, and read the fluorescence intensity in endpoint mode with excitation and emission wavelengths of 345 nm and 395 nm (top read).
2. Thaw the frozen substrate at 37°C for 5 minutes and mix well.
3. Dilute Mouse PLA2G1B protein to 100 μg/mL in assay buffer.
4. Dilute trypsin to 0.5 μg/mL in assay buffer.
5. Mixture preparation: Mix equal volumes of diluted trypsin and Mouse PLA2G1B protein, and incubate at room temperature for 30 minutes.
6. Dilute the substrate to 100 μM in substrate buffer.
Experimental group: 50 μL mixture + 50 μL substrate.
Blank group: 50 μL assay buffer + 50 μL substrate.
7. Read in kinetic mode for 5 minutes with excitation and emission wavelengths of 345 nm and 395 nm (top read) respectively.
8. Calculate the specific activity:

Mouse

HEK293

C-6*His

Q9Z0Y2 (A16-C146)

18778  [NCBI]

Full Length of Mature Protein

AHSISPRAVWQFRNMIKCTIPGSDPLKDYNNYGCYCGLGGWGTPVDDLDRCCQTHDHCYSQAKKLESCKFLIDNPYTNTYSYSCSGSEITCSAKNNKCEDFICNCDREAAICFSKVPYNKEYKNLDTGKFC

Approximately 15.0 kDa

• 
  Greater than 95% as determined by reducing SDS-PAGE.

溶液.

Supplied as a 0.22 μm filtered solution of 20 mM HEPES, 150 mM NaCl, pH 7.0.

<1 EU/μg, determined by LAL method.

N/A.

Stored at -80°C for 1 year from date of receipt. It is stable at -20°C for 3 months after opening. It is recommended to freeze aliquots at -80°C for extended storage. Avoid repeated freeze-thaw cycles.

Shipping with dry ice.