蛋白 E7, HPV (His)

HPV protein E7 can induce dormant cells to enter the cell cycle and promote the efficient use of cellular DNA replication mechanisms for viral genome replication. Through translational and transactivation activities, E7 disrupts the RB1-E2F1 complex and activates E2F1-regulated S-phase genes. Protein E7, HPV (His) is the recombinant Virus-derived protein E7, HPV, expressed by E. coli , with N-6*His labeled tag. The total length of Protein E7, HPV (His) is 98 a.a., with molecular weight of ~21 kDa.

人乳头瘤病毒 (HPV) 的 E7 蛋白通过诱导静止细胞进入细胞周期，在病毒基因组复制中发挥着至关重要的作用，从而促进细胞 DNA 复制机制在病毒基因组复制中的有效利用。E7 表现出转化和反式激活活性，破坏 RB1-E2F1 复合物并激活 E2F1 调节的 S 期基因。它干扰 HDAC1 和 HDAC2 介导的宿主组蛋白脱乙酰化，从而导致转录激活。此外，E7 还能抑制宿主干扰素 α 的抗病毒和抗增殖功能。它与宿主 TMEM173/STING 的相互作用阻碍了 TMEM173/STING 感知胞质 DNA 和促进 I 型干扰素产生的能力。E7 形成同源二聚体和同源寡聚体，与宿主 RB1 相互作用以破坏 RB1 活性，并与 EP300 结合以抑制 EP300 转录活性。与 CHD4 和 HDAC1 形成的复合物会改变宿主组蛋白脱乙酰化，而与蛋白质 E2 的相互作用会抑制 E7 致癌活性。蛋白质 E7 的多方面作用强调了其在 HPV 相关细胞过程中的关键作用及其对宿主细胞功能的复杂调节。

Measured by its binding ability in a functional ELISA. Immobilized Protein E7 at 10 μg/mL (100 μL/well) can bind Biotinylated MYC. The ED₅₀ for this effect is 0.2398 μg/mL, corresponding to a specific activity is 4.17×10³ Unit/mg.

• 
  Measured by its binding ability in a functional ELISA. Immobilized Protein E7 at 10 μg/mL (100 μL/well) can bind Biotinylated MYC. The ED₅₀ for this effect is 0.2398 μg/mL, corresponding to a specific activity is 4.17×10³ Unit/mg.

Virus

E. coli

N-6*His

P03129 (M1-P98)

1489079  [NCBI]

MHGDTPTLHEYMLDLQPETTDLYCYEQLNDSSEEEDEIDGPAGQAEPDRAHYNIVTFCCKCDSTLRLCVQSTHVDIRTLEDLLMGTLGIVCPICSQKP

Approximately 21 kDa

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  Greater than 94% as determined by reducing SDS-PAGE.

无菌粉末

Lyophilized from 0.2 μm filtered solution in 20 mM Tris-HCL, 0.5 M NaCl, 6% Trehalose, pH 8.0 or 50 mM Tris-HCL, 300 mM NaCl, pH 7.4 or PBS, 6% Trehalose, pH 7.4.

<1 EU/μg, determined by LAL method.

It is not recommended to reconstitute to a concentration less than 100 μg/mL in ddH₂O. For long term storage it is recommended to add a carrier protein (0.1% BSA, 5% HSA, 10% FBS or 5% Trehalose).

Stored at -20°C for 2 years. After reconstitution, it is stable at 4°C for 1 week or -20°C for longer (with carrier protein). It is recommended to freeze aliquots at -20°C or -80°C for extended storage.

Room temperature in continental US; may vary elsewhere.