SARS-CoV S 蛋白 (S577A, sf9, His)

SARS-CoV spike glycoprotein 2 (S2) is a subunit of SARS-CoV Spike glycoprotein, among with S1 and S2'. S2 (668-1255) mediates fusion of the virion and cellular membranes by acting as a class I viral fusion protein, during endocytosis, S2 is cleaved into S2' (798-1255). Internalization into host cell endosomes induces S glycoprotein conformational changes, potentially unmasking the fusion peptide of S2 through cathepsin CTSL proteolysis. SARS-CoV S Protein (S577A, sf9, His) is the recombinant Virus-derived SARS-CoV S protein, expressed by Sf9 insect cells , with C-His labeled tag. The total length of SARS-CoV S Protein (S577A, sf9, His) is 1195 a.a., with molecular weight of ~132.58 kDa.

SARS-CoV 刺突糖蛋白 2 (S2) 是 SARS-CoV 刺突糖蛋白的一个亚基，与 S1 和 S2' 并列。S1 (14-667) 可以通过与宿主受体相互作用将病毒粒子附着在细胞膜上，引发感染。S2 (668-1255) 作为一类病毒融合蛋白介导病毒粒子与细胞膜的融合，在内吞过程中，S2 被裂解成 S2' (798-1255)。
S 蛋白通过与人类 ACE2 和 CLEC4M/DC-SIGNR 受体的相互作用，将病毒粒子附着在细胞膜上，从而协调病毒进入。附着后，内化到宿主细胞内体诱导 S 糖蛋白构象改变，从而通过组织蛋白酶 CTSL 蛋白水解揭示 S2 融合肽^[1][2]。

Virus

Sf9 insect cells

C-His

NP_828851.1 (M1-P1195)

1489668  [NCBI]

Approximately 132.58 kDa

Greater than 90% as determined by reducing SDS-PAGE.

<1 EU/μg, determined by LAL method.