Semaphorin-4A/SEMA4A 蛋白, Mouse (HEK293, His)

Semaphorin-4A/SEMA4A is the cell surface receptor for PLXNB1, PLXNB2, PLXNB3 and PLXND1 and is critical for intercellular signaling. It regulates synaptic development, promoting inhibitory synapses via PLXNB1 and excitatory synapses via PLXNB2. Semaphorin-4A/SEMA4A Protein, Mouse (HEK293, His) is the recombinant mouse-derived Semaphorin-4A/SEMA4A protein, expressed by HEK293 , with C-6*His labeled tag. The total length of Semaphorin-4A/SEMA4A Protein, Mouse (HEK293, His) is 650 a.a., with molecular weight of 67-82 kDa.

Semaphorin-4A/SEMA4A 蛋白作为 PLXNB1、PLXNB2、PLXNB3 和 PLXND1 的细胞表面受体，在细胞间信号传导中发挥着至关重要的作用。它参与调节谷氨酸能和 GABA 能突触发育，强调了它在突触过程中的重要性。SEMA4A 以 PLXNB1 依赖性方式促进抑制性突触发育，并以 PLXNB2 依赖性方式促进兴奋性突触发育。除了突触功能之外，SEMA4A 还通过启动抗原特异性 T 细胞和促进 Th1 T 辅助细胞的分化来促进适应性免疫。此外，SEMA4A 促进 TIMD2 磷酸化并抑制血管生成。它在轴突生长锥塌陷和抑制轴突延伸中的作用进一步凸显了它在提供局部信号以指定生长轴突无法到达的区域方面的影响。该蛋白与 PLXNB1、PLXNB2、PLXNB3、PLXND1 和 TIMD2 相互作用，展示了其在多种细胞过程和信号通路中的多功能参与。

Immobilized Mouse Semaphorin 4A at 2 μg/mL (100 μL/well) can bind Biotinylated Human ILT4 protein. The ED₅₀ for this effect is 0.6023 μg/mL.

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  Immobilized Mouse Semaphorin 4A at 2 μg/mL (100 μL/well) can bind Biotinylated Human ILT4 protein. The ED₅₀ for this effect is 0.6023 μg/mL.

Mouse

HEK293

C-6*His

Q62178 (T33-H682)

20351  [NCBI]

TGGQGPMPRVKYHAGDGHRALSFFQQKGLRDFDTLLLSDDGNTLYVGAREAVLALNIQNPGIPRLKNMIPWPASERKKTECAFKKKSNETQCFNFIRVLVSYNATHLYACGTFAFSPACTFIELQDSLLLPILIDKVMDGKGQSPFDPVHKHTAVLVDGMLYSGTMNNFLGSEPILMRTLGSQPVLKTDIFLRWLHADASFVAAIPSTQVVYFFFEETASEFDFFEELYISRVAQVCKNDVGGEKLLQKKWTTFLKAQLLCAQPGQLPFNIIRHAVLLPADSPSVSRIYAVFTSQWQVGGTRSSAVCAFSLTDIERVFKGKYKELNKETSRWTTYRGSEVSPRPGSCSMGPSSDKALTFMKDHFLMDEHVVGTPLLVKSGVEYTRLAVESARGLDGSSHVVMYLGTSTGSLHKAVVPQDSSAYLVEEIQLSPDSEPVRNLQLAPAQGAVFAGFSGGIWRVPRANCSVYESCVDCVLARDPHCAWDPESRLCSLLSGSTKPWKQDMERGNPEWVCTRGPMARSPRRQSPPQLIKEVLTVPNSILELPCPHLSALASYHWSHGRAKISEASATVYNGSLLLLPQDGVGGLYQCVATENGYSYPVVSYWVDSQDQPLALDPELAGVPRERVQVPLTRVGGGASMAAQRSYWPH

67-82 kDa

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  Greater than 95% as determined by reducing SDS-PAGE.

无菌粉末.

Lyophilized from a 0.2 μm filtered solution of 20 mM PB, 150 mM NaCl, 1mM EDTA, 5% Trehalose, pH 7.4 or 20 mM PB, 150 mM NaCl, 1 mM EDTA, pH 7.4.

<1 EU/μg, determined by LAL method.

It is not recommended to reconstitute to a concentration less than 100 μg/mL in ddH₂O. For long term storage it is recommended to add a carrier protein (0.1% BSA, 5% HSA, 10% FBS or 5% Trehalose).

Stored at -20°C for 2 years. After reconstitution, it is stable at 4°C for 1 week or -20°C for longer (with carrier protein). It is recommended to freeze aliquots at -20°C or -80°C for extended storage.

Room temperature in continental US; may vary elsewhere.