1. 重组蛋白
  2. Enzymes & Regulators
  3. Protease Inhibitors
  4. Serpin (Protease Inhibitor)
  5. PAI-1
  6. Serpin E1 蛋白, Human (HEK293, His)

Serpin E1 蛋白, Human (HEK293, His)

目录号: HY-P71077
COA 产品使用指南

Serpin E1 是一种丝氨酸蛋白酶抑制剂,通过抑制 TMPRSS7、组织型纤溶酶原激活剂 (PLAT) 和尿激酶型纤溶酶原激活剂 (PLAU) 来调节各种细胞过程。在纤维蛋白溶解过程中,它下调 PLAT 以控制血栓降解,并作为 PLAU 抑制剂调节细胞粘附。Serpin E1 蛋白, Human (HEK293, His) 是重组的 丝氨酸蛋白酶抑制剂 E1 蛋白,由 HEK293 表达,带有 C-6*His 标签。Serpin E1 蛋白, Human (HEK293, His) 全长 379 个氨基酸,分子量约为 ~44-51 kDa。

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描述

Serpin E1 is a serine protease inhibitor that regulates various cellular processes by inhibiting TMPRSS7, tissue-type plasminogen activator (PLAT), and urokinase-type plasminogen activator (PLAU). During fibrinolysis, it downregulates PLAT to control thrombus degradation and acts as a PLAU inhibitor to regulate cell adhesion. Serpin E1 Protein, Human (HEK293, His) is the recombinant human-derived Serpin E1 protein, expressed by HEK293 , with C-6*His labeled tag. The total length of Serpin E1 Protein, Human (HEK293, His) is 379 a.a., with molecular weight of ~44-51 kDa.

研究背景

Serpin E1 是一种丝氨酸蛋白酶抑制剂,对多种细胞过程发挥调节作用。它抑制 TMPRSS7,并作为组织型纤溶酶原激活剂 (PLAT) 和尿激酶型纤溶酶原激活剂 (PLAU) 的主要抑制剂。在纤溶过程中,Serpin E1 对于下调 PLAT 至关重要,有助于控制血栓的降解。同时,作为 PLAU 抑制剂,它在调节细胞粘附和扩散方面发挥作用。除了作为蛋白酶抑制剂的功能外,Serpin E1 还充当细胞迁移的调节剂,影响皮肤损伤修复过程中的角质形成细胞迁移等过程。它的参与延伸到细胞和复制衰老,导致肺部 2 型肺泡细胞衰老。此外,Serpin E1参与牙周膜干细胞牙骨质形成分化的调节,并影响成牙本质细胞分化和牙本质形成。该蛋白参与各种相互作用,与 TMPRSS7 形成异二聚体,并与多个伙伴相互作用,包括 VTN、PPP1CB、PLAUR/uPAR、SORL1 和 LRP1 等,突出了其在不同细胞途径中的多方面作用。

生物活性

Measured by its ability to inhibit uPA cleavage of a peptide substrate, N-carbobenzyloxy-Gly-Gly-Arg-7-amido-4-methylcoumarin (Z-GGR-AMC). The IC50 value is <15 nM, as measured under the described conditions.

种属

Human

表达系统

HEK293

标签

C-6*His

蛋白编号

P05121 (V24-P402)

基因 ID
蛋白结构
N-term
Serpin E1 (V24-P402)
Accession # P05121
6*His
C-term
同用名
Plasminogen Activator Inhibitor 1; PAI; PAI-1; Endothelial Plasminogen Activator Inhibitor; Serpin E1; SERPINE1; PAI1; PLANH1
氨基酸序列

VHHPPSYVAHLASDFGVRVFQQVAQASKDRNVVFSPYGVASVLAMLQLTTGGETQQQIQAAMGFKIDDKGMAPALRHLYKELMGPWNKDEISTTDAIFVQRDLKLVQGFMPHFFRLFRSTVKQVDFSEVERARFIINDWVKTHTKGMISNLLGKGAVDQLTRLVLVNALYFNGQWKTPFPDSSTHRRLFHKSDGSTVSVPMMAQTNKFNYTEFTTPDGHYYDILELPYHGDTLSMFIAAPYEKEVPLSALTNILSAQLISHWKGNMTRLPRLLVLPKFSLETEVDLRKPLENLGMTDMFRQFQADFTSLSDQEPLHVAQALQKVKIEVNESGTVASSSTAVIVSARMAPEEIIMDRPFLFVVRHNPTGTVLFMGQVMEP

分子量

Approximately 44-51 kDa

纯度
  • Greater than 95% as determined by reducing SDS-PAGE.
性状

无菌粉末.

组分

Lyophilized from a 0.2 μm filtered solution of 20 mM HAc-NaAc, 150 mM NaCl, pH 4.0 or 20 mM HAC-NaAC, 150 mM NaCl, pH 5.5.

内毒素含量

<1 EU/μg, determined by LAL method.

复溶方法

It is not recommended to reconstitute to a concentration less than 100 μg/mL in ddH2O. For long term storage it is recommended to add a carrier protein (0.1% BSA, 5% HSA, 10% FBS or 5% Trehalose).

保存条件 & 期限

Stored at -20°C for 2 years. After reconstitution, it is stable at 4°C for 1 week or -20°C for longer (with carrier protein). It is recommended to freeze aliquots at -20°C or -80°C for extended storage.

运输条件

Room temperature in continental US;may vary elsewhere.

文件资料

Serpin E1 Protein, Human (HEK293, His) 相关分类

Help & FAQs
  • Do most proteins show cross-species activity?

    Species cross-reactivity must be investigated individually for each product. Many human cytokines will produce a nice response in mouse cell lines, and many mouse proteins will show activity on human cells. Other proteins may have a lower specific activity when used in the opposite species.

  • 复溶计算器

  • 重组蛋白稀释计算器

  • 比活力计算器

The reconstitution calculator equation

Volume (to add to vial) = Mass (in vial) ÷ Desired Reconstitution Concentration

Volume (to add to vial) = Mass (in vial) ÷ Desired Reconstitution Concentration
= ÷

The dilution calculator equation

Concentration (start) × Volume (start) = Concentration (final) × Volume (final)

This equation is commonly abbreviated as: C1V1 = C2V2

浓度 (start) × 体积 (start) = 浓度 (final) × 体积 (final)
× = ×
C1   V1   C2   V2

The specific activity calculator equation

Specific Activity (Unit/mg) = 106 ÷ Biological Activity (ED50)

Specific Activity (Unit/mg) = 106 ÷ Biological Activity (ED50)
Unit/mg = 106 ÷ ng/mL

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产品名称:
Serpin E1 Protein, Human (HEK293, His)
目录号:
HY-P71077
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