SFRP1 蛋白, Human (HEK293, His)

SFRP1 protein is a soluble Frizzled-related protein (sFRP) that regulates Wnt signaling by directly interacting with Wnt, affecting cell growth and differentiation. Notably, it reduces intracellular β-catenin levels and affects Wnt pathway components. SFRP1 Protein, Human (HEK293, His) is the recombinant human-derived SFRP1 protein, expressed by HEK293 , with C-His labeled tag. The total length of SFRP1 Protein, Human (HEK293, His) is 283 a.a., with molecular weight of ~38 kDa.

SFRP1 是一种可溶性卷曲相关蛋白 (sFRP)，通过与 Wnt 直接相互作用充当 Wnt 信号传导调节剂，有助于调节特定细胞类型的细胞生长和分化。值得注意的是，SFRP1 在降低细胞内 β-连环蛋白水平方面发挥作用，表明其对 Wnt 通路成分的影响。除了参与 Wnt 信号传导之外，SFRP1 在体外和体内均表现出对血管细胞的抗增殖作用，诱导体内血管生成反应。在血管细胞周期中，它延迟G1期并进入S期。在肾脏发育过程中，SFRP1 抑制后肾小管形成和芽生长。此外，它还阻碍 WNT1/WNT4 介导的 TCF 依赖性转录，并与 WNT1、WNT2、FRZD6、WNT4、WNT8 和 MYOC 相互作用，强调其在细胞过程和分子相互作用中的多方面作用。

Measured by its ability to inhibit proliferation of HeLa human cervical epithelial carcinoma cells and the ED₅₀ is typically 5-30 μg/mL.

Human

HEK293

C-His

Q8N474 (S32-K314)

6422  [NCBI]

Approximately 38 kDa

Greater than 95% as determined by reducing SDS-PAGE

无菌粉末.

Lyophilized from a 0.2 μm filtered solution of PBS, pH 7.4. Normally 5 % - 8 % trehalose, mannitol and 0.01% Tween 80 are added as protectants before lyophilization.

<1 EU/μg, determined by LAL method.

It is not recommended to reconstitute to a concentration less than 100 μg/mL in ddH₂O.

Stored at -20°C for 2 years. After reconstitution, it is stable at 4°C for 1 week or -20°C for longer (with carrier protein). It is recommended to freeze aliquots at -20°C or -80°C for extended storage.

Room temperature in continental US; may vary elsewhere.