Siglec-9 蛋白, Human (HEK293, His)

Siglec-9 Protein is a member of the Siglec cell surface immunoglobulin family. Siglec-9 is highly expressed on human neutrophils and monocytes and low on natural killer cells, and sub-populations of T and B lymphocytes. Siglec-9 induces human neutrophil apoptosis and autophagy-like cell death and also inhibits tumor activity. Siglec-9 Protein, Human (HEK293, His) is the recombinant human-derived Siglec-9 protein, expressed by HEK293 , with C-6*His labeled tag. The total length of Siglec-9 Protein, Human (HEK293, His) is 331 a.a., with molecular weight of 55-90 kDa.

唾液酸结合免疫球蛋白样凝集素 (SIGLEC9) 是 Siglec 细胞表面免疫球蛋白家族的成员。Siglec-9 在人类中性粒细胞和单核细胞中高表达，在自然杀伤细胞以及 T 和 B 淋巴细胞亚群中低表达。通过化学化合物或合成配体连接 Siglec-9 可诱导人中性粒细胞凋亡和自噬样细胞死亡。Siglec-9 与唾液酸结合并将凋亡和非凋亡死亡信号转导至中性粒细胞。Siglec-9 是巨噬细胞上的一种免疫检查点分子，可靶向增强 GBM 治疗的抗 PD-1/PD-L1 治疗效果。通过阻断 Siglec-9 可以抑制促肿瘤活性，Siglec-9 释放抗肿瘤功能并诱导强大的肿瘤消除活性。靶向 Siglec-9 可能有益于治疗慢性阻塞性肺病、哮喘、纤维化和相关的慢性炎症性肺部疾病^[1][2][3]。

Human

HEK293

C-6*His

AAH35365.2 (Q18-G348)

27180  [NCBI]

QTSKLLTMQSSVTVQEGLCVHVPCSFSYPSHGWIYPGPVVHGYWFREGANTDQDAPVATNNPARAVWEETRDRFHLLGDPHTKNCTLSIRDARRSDAGRYFFRMEKGSIKWNYKHHRLSVNVTALTHRPNILIPGTLESGCPQNLTCSVPWACEQGTPPMISWIGTSVSPLDPSTTRSSVLTLIPQPQDHGTSLTCQVTFPGASVTTNKTVHLNVSYPPQNLTMTVFQGDGTVSTVLGNGSSLSLPEGQSLRLVCAVDAVDSNPPARLSLSWRGLTLCPSQPSNPGVLELPWVHLRDAAEFTCRAQNPLGSQQVYLNVSLQSKATSGVTQG

55-90 kDa

Greater than 95% as determined by reducing SDS-PAGE.

无菌粉末.

Lyophilized from a 0.2 μm filtered solution of PBS, 2 mM EDTA, pH 7.4.

<1 EU/μg, determined by LAL method.

It is not recommended to reconstitute to a concentration less than 100 μg/mL in ddH₂O. For long term storage it is recommended to add a carrier protein (0.1% BSA, 5% HSA, 10% FBS or 5% Trehalose).

Stored at -20°C for 2 years. After reconstitution, it is stable at 4°C for 1 week or -20°C for longer (with carrier protein). It is recommended to freeze aliquots at -20°C or -80°C for extended storage.

Room temperature in continental US;may vary elsewhere.