TCPTP 蛋白, Human (sf9)

TCPTP, a non-receptor tyrosine-specific phosphatase, critically regulates various kinases (INSR, EGFR, JAKs, Src), influencing hematopoiesis, inflammation, cell proliferation, and glucose homeostasis. In the immune system, TCPTP modulates T-cell receptor signaling, inhibiting cytokine pathways (IL2, interferon). It negatively regulates EGF-stimulated cell growth, PDGF receptor-beta signaling, and insulin receptor signaling, impacting glucose homeostasis and liver glucose production through the IL6 pathway. TCPTP's broad influence emphasizes its pivotal role in diverse cellular processes. TCPTP Protein, Human (sf9) is the recombinant human-derived TCPTP protein, expressed by Sf9 insect cells , with tag free. and with residual Gly-Pro in N-terminal (not related to functional changes) mutation. The total length of TCPTP Protein, Human (sf9) is 314 a.a., with molecular weight of ~37 kDa.

TCPTP 是一种非受体型酪氨酸特异性磷酸酶，通过去磷酸化各种受体和非受体蛋白酪氨酸激酶（包括 INSR、EGFR、CSF1R、PDGFR、JAK1、JAK2、JAK3、Src 家族激酶、STAT1、 STAT3和STAT6位于细胞核或细胞质中。这种磷酸酶对多种信号通路和生物过程（例如造血、炎症反应、细胞增殖和分化以及葡萄糖稳态）发挥负控制。在免疫系统中，TCPTP 发挥多方面的作用，通过使 FYN 和 LCK 去磷酸化来影响 T 细胞受体信号传导，从而调节 T 细胞分化和激活。此外，它还负向调节细胞因子介导的信号传导，包括 IL2、干扰素、IL6 和 IL4 途径。除了免疫调节之外，TCPTP 还参与 EGF 刺激的细胞生长和 PDGF 受体 β 信号传导的锚定依赖性负调节，从而影响细胞增殖。此外，它在葡萄糖稳态中发挥着至关重要的作用，负向调节胰岛素受体信号通路，并通过IL6信号通路控制糖异生和肝脏葡萄糖的产生。 TCPTP 的广泛影响强调了它在多种细胞过程中的重要性，并且它还可能具有 DNA 结合能力。

Human

Sf9 insect cells

Tag Free

P17706 (N-G&P, M1-N314)

5771  [NCBI]

Approximately 37 kDa

Greater than 95% as determined by reducing SDS-PAGE

<1 EU/μg, determined by LAL method.