UBE2D3 蛋白, Human

Ubiquitin-conjugating enzyme E2 D3 (UBE2D3) is an E3 ubiquitin protein ligase. UBE2D3 is able to accept ubiquitin from specific E2 ubiquitin-conjugating enzymes and transfer it to substrates, often promoting its degradation by the proteasome^[1]. UBE2D3 Protein, Human is the recombinant human-derived UBE2D3 protein, expressed by E. coli , with tag free. The total length of UBE2D3 Protein, Human is 147 a.a., with molecular weight of ~15.0 kDa.

用泛素修饰蛋白质是针对异常或短寿命蛋白质进行降解的重要细胞机制。泛素化至少涉及三类酶：泛素激活酶（E1）、泛素结合酶（E2）和泛素蛋白连接酶（E3）。UBE2D3 编码 E2 泛素结合酶家族的成员，在肿瘤中抑制蛋白 p53 的泛素化过程。UBE2D3 蛋白由 E3 泛素蛋白连接酶诱导，在肿瘤发生中发挥重要调节作用。特别是 UBE2D3 在胶质瘤中高表达，可能是神经胶质瘤治疗的潜在靶点。UBE2D3 可以促进 SHP-2 的泛素化，从而激活 STAT3 通路并促进胶质瘤增殖和糖酵解。UBE2D3 可以与 SHP-2 相互作用并促进其泛素化，从而提高 STAT3 通路的激活。抑制 UBE2D3 可以在体外和体内抑制 GBM 的增殖、糖酵解和 STAT3 磷酸化^[1][2]。

The enzyme activity of this recombinant protein is testing in progress, we cannot offer a guarantee yet.

Human

E. coli

Tag Free

AAH66917 (M1-M147)

7323  [NCBI]

MALKRINKELSDLARDPPAQCSAGPVGDDMFHWQATIMGPNDSPYQGGVFFLTIHFPTDYPFKPPKVAFTTRIYHPNINSNGSICLDILRSQWSPALTISKVLLSICSLLCDPNPDDPLVPELARIYKTDRDKYNRISREWTQKYAM

Approximately 15.0 kDa

Greater than 95% as determined by reducing SDS-PAGE.

溶液

Supplied as a 0.2 μm filtered solution of 20 mM Tris-HCl, 150 mM NaCl, 50% Glycerol, 8% Sucrose, 0.05% Tween 80, pH 7.1.

<1 EU/μg, determined by LAL method.

Stored at -80°C for 1 year. It is stable at -20°C for 3 months after opening. It is recommended to freeze aliquots at -80°C for extended storage. Avoid repeated freeze-thaw cycles.

Shipping with dry ice.