1. 重组蛋白
  2. Cytokines and Growth Factors CAR-T related Proteins Receptor Proteins Enzymes & Regulators
  3. VEGF & VEGFR Receptor Tyrosine Kinases Protein Tyrosine Kinases
  4. VEGFR
  5. FLK-1/VEGFR-2 FLK-1/VEGFR-2
  6. VEGFR-2 蛋白, Mouse (HEK293, His)

VEGFR-2 蛋白, Mouse (HEK293, His)

目录号: HY-P74471
产品使用指南

VEGFR-2蛋白是一种重要的酪氨酸蛋白激酶受体。作为VEGFA、VEGFC和VEGFD的细胞表面受体,在血管生成、血管发育、通透性和胚胎造血的调节中发挥重要作用。它积极促进内皮细胞增殖、存活、迁移、分化和肌动蛋白细胞骨架重组。VEGFR-2 蛋白, Mouse (HEK293, His) 是重组的 VEGFR-2 蛋白,由 HEK293 表达,带有 C-His 标签。VEGFR-2 蛋白, Mouse (HEK293, His) 全长 762 个氨基酸,分子量为 100-110 kDa。

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描述

VEGFR-2 protein is an important tyrosine protein kinase receptor. As a cell surface receptor for VEGFA, VEGFC and VEGFD, it plays an important role in the regulation of angiogenesis, vascular development, permeability and embryonic hematopoiesis. It actively promotes endothelial cell proliferation, survival, migration, differentiation, and actin cytoskeletal reorganization. VEGFR-2 Protein, Mouse (HEK293, His) is the recombinant mouse-derived VEGFR-2 protein, expressed by HEK293 , with C-His labeled tag. The total length of VEGFR-2 Protein, Mouse (HEK293, His) is 762 a.a., with molecular weight of 100-110 kDa.

研究背景

VEGFR-2 是一种酪氨酸蛋白激酶,是 VEGFA、VEGFC 和 VEGFD 的重要细胞表面受体,在血管生成调节、血管发育、通透性和胚胎造血中发挥重要作用。它积极促进内皮细胞增殖、存活、迁移和分化,以及肌动蛋白细胞骨架的重组。缺乏跨膜结构域的异构体(如异构体 2)可充当诱饵受体,特别是通过限制游离 VEGFA 和/或 VEGFC 的可用性并阻止其与 FLT4 结合来负调节 VEGFA 和 VEGFC 介导的淋巴管生成。VEGFR-2 通过异二聚体形成调节 FLT1 和 FLT4 信号传导。血管生长因子与异构体 1 的结合启动各种信号级联反应,包括 PLCG1 的激活,导致二酰基甘油和肌醇 1,4,5-三磷酸的产生以及蛋白激酶 C 的激活。此外,它还介导MAPK1/ERK2、MAPK3/ERK1、AKT1 和 PIK3R1 的磷酸化,有助于磷脂酰肌醇 3-激酶调节和肌动蛋白细胞骨架重组。VEGFR-2 对于 VEGFA 诱导的 NOS2 和 NOS3 表达不可或缺,从而导致内皮细胞产生一氧化氮,并且它磷酸化 PLCG1、FYN、NCK1、NOS3、PIK3R1、PTK2/FAK1 和 SRC

种属

Mouse

表达系统

HEK293

标签

C-His

蛋白编号

P35918 (M1-E762)

基因 ID
蛋白结构
N-term
VEGFR-2 (M1-E762)
Accession # P35918
His
C-term
同用名
Vascular endothelial growth factor receptor 2; VEGFR-2; FLK-1; CD309
分子量

100-110 kDa

纯度

Greater than 90% as determined by reducing SDS-PAGE

内毒素含量

<1 EU/μg, determined by LAL method.

文件资料
Help & FAQs
  • Do most proteins show cross-species activity?

    Species cross-reactivity must be investigated individually for each product. Many human cytokines will produce a nice response in mouse cell lines, and many mouse proteins will show activity on human cells. Other proteins may have a lower specific activity when used in the opposite species.

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The reconstitution calculator equation

Volume (to add to vial) = Mass (in vial) ÷ Desired Reconstitution Concentration

Volume (to add to vial) = Mass (in vial) ÷ Desired Reconstitution Concentration
= ÷

The dilution calculator equation

Concentration (start) × Volume (start) = Concentration (final) × Volume (final)

This equation is commonly abbreviated as: C1V1 = C2V2

浓度 (start) × 体积 (start) = 浓度 (final) × 体积 (final)
× = ×
C1   V1   C2   V2

The specific activity calculator equation

Specific Activity (Unit/mg) = 106 ÷ Biological Activity (ED50)

Specific Activity (Unit/mg) = 106 ÷ Biological Activity (ED50)
Unit/mg = 106 ÷ ng/mL

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VEGFR-2 Protein, Mouse (HEK293, His)
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