Rhodamine-N3 chloride 

Rhodamine-N3 chloride is an azide-rhodamine fluorescent dye that can be used to label biomolecules containing alkyne groups^[1][2]. Rhodamine-N3 (chloride) is a click chemistry reagent, it contains an Azide group and can undergo copper-catalyzed azide-alkyne cycloaddition reaction (CuAAc) with molecules containing Alkyne groups. Strain-promoted alkyne-azide cycloaddition (SPAAC) can also occur with molecules containing DBCO or BCN groups.

细胞裂解物或细胞用探针 (0.001-10 μM，1 小时) 处理，然后在铜催化的叠氮化物-炔烃环加成 (CuACC 或点击化学) 条件下与罗丹明-N3 (N3-Rh) 报告标签反应，然后通过 SDS-PAGE 和凝胶内荧光扫描显示探针标记的蛋白质^[2]。

MCE has not independently confirmed the accuracy of these methods. They are for reference only.

847.44

C₄₄H₅₉ClN₈O₇

2363751-90-8

固体

Purple to purplish red

576

544

Room temperature in continental US; may vary elsewhere.

4°C, sealed storage, away from moisture and light

*In solvent : -80°C, 6 months; -20°C, 1 month (sealed storage, away from moisture and light)

* 请根据产品在不同溶剂中的溶解度选择合适的溶剂配制储备液；一旦配成溶液，请分装保存，避免反复冻融造成的产品失效。
储备液的保存方式和期限：-80°C, 6 months; -20°C, 1 month (sealed storage, away from moisture and light)。-80°C储存时，请在6个月内使用，-20°C储存时，请在1个月内使用。

• [1]. Shijiao Cai, et al. Glycyrrhizic Acid-Induced Differentiation Repressed Stemness in Hepatocellular Carcinoma by Targeting c-Jun N-Terminal Kinase 1. Front Oncol. 2020 Jan 9;9:1431.  [Content Brief]

  [2]. Bryan R Lanning, et al. A road map to evaluate the proteome-wide selectivity of covalent kinase inhibitors. Nat Chem Biol. 2014 Sep;10(9):760-767.  [Content Brief]