Sialidase (α2-3-6-8-9)  (Synonyms: 唾液酸酶（α2-3-6-8-9）)

Sialidase (α2-3-6-8-9) is a broadly specific sialidase that cuts linear and branched non-reducing terminal sialic acid residues from glycoproteins, glycopeptides, and oligosaccharides. Sialidase (α2-3-6-8-9) can be used for in vitro and in vivo polysaccharide analysis and characterization as well as complete glycoprotein remodeling^[1].

产品信息
本产品由重组大肠杆菌表达。该酶可水解糖蛋白和寡糖上通过 α2-3、α2-6、α2-8 和 α2-9 键连接的唾液酸。α2-3 和 α2-6 键的水解速率高于 α2-8 和 α2-9 键。
储存缓冲液：20 mM Tris，50 mM NaCl，1 mM EDTA，pH 8.0

操作步骤
1. 根据反应体系，加入底物 (2 μg 糖蛋白或 200 nM 寡糖)、1.0 μL 唾液酸酶 (α2-3,6,8,9)、2.5 μL 反应缓冲液 (50 mM CaCl₂，500 mM 乙酸钠，pH 5.5)，并加水至 25 μL；
2. 混合后，将样品于 37°C 孵育 5-60 分钟。然后于 65°C 加热 10 分钟以终止反应。

注意事项
上述唾液酸酶反应缓冲液的 pH 值为 5.5。如果想用酶法裂解细胞表面的唾液酸，请更换与细胞相容的缓冲液体系。

MCE has not independently confirmed the accuracy of these methods. They are for reference only.

液体

Colorless to light yellow

唾液酸酶（α2-3-6-8-9）

≥50 U/μL

One unit is defined as the amount of enzyme that will cut >95% of terminal α-Neu5Ac from 1 nmol Neu5Acα2-3Galβ1-3GlcNAcβ1-3Galβ1-4Glc-7-amino-4-methyl-coumarin (AMC) in a total reaction volume of 10 μL at 37℃ within 5 min.

Room temperature in continental US; may vary elsewhere.

Please store the product under the recommended conditions in the Certificate of Analysis.

• [1]. Means RE, Desrosiers RC. Resistance of native, oligomeric envelope on simian immunodeficiency virus to digestion by glycosidases. J Virol. 2000 Dec;74(23):11181-90.  [Content Brief]