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  3. Fluorescent Dye
  4. Tetramethylrhodamine-5-iodoacetamide

Tetramethylrhodamine-5-iodoacetamide  (Synonyms: 5-TMRIA)

目录号: HY-123749
产品使用指南

摩尔计算器

Tetramethylrhodamine-5-iodoacetamide (5-TMRIA) 是一种选择性巯基反应染料，用于通过半胱氨酸残基对蛋白质进行非特异性标记。Tetramethylrhodamine-5-iodoacetamide (5-TMRIA) 可用于共价标记 DNA 片段。

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Tetramethylrhodamine-5-iodoacetamide Chemical Structure

Tetramethylrhodamine-5-iodoacetamide Chemical Structure

CAS No. : 114458-99-0

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Tetramethylrhodamine-5-iodoacetamide 相关抗体

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  • 生物活性

  • 纯度 & 产品资料

  • 参考文献

生物活性

Tetramethylrhodamine-5-iodoacetamide (5-TMRIA) is a thiol-selective reactive dye that is used to non-specifically label proteins via the cysteine residues. Tetramethylrhodamine-5-iodoacetamide (5-TMRIA) can be used to covalently label DNA fragments[1].

体外研究
(In Vitro)

Tetramethylrhodamine-5-iodoacetamide (0.5, 1.0, 2.0, 3.0, 4.0, 5.0, and 7.5 mM dye) shows different results for labeling carbonic anhydrase and myoglobin. At lower dye concentrations (up to 40:1 dye:thiol ratio) fluorescence labeling of only the carbonic anhydrase band is observed. However,as concentration increases, fluorescence labeling of both carbonic anhydrase and the lower myoglobin band is observed, indicating nonspecific labeling at these dye:thiol ratios[1].

MCE has not independently confirmed the accuracy of these methods. They are for reference only.

Tetramethylrhodamine-5-iodoacetamide 相关抗体:
分子量

569.39

Formula

C26H24IN3O4
CAS 号
运输条件

Room temperature in continental US; may vary elsewhere.
储存方式

Please store the product under the recommended conditions in the Certificate of Analysis.
纯度 & 产品资料
参考文献

Tetramethylrhodamine-5-iodoacetamide 相关分类

  • 摩尔计算器

  • 稀释计算器

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  • Do most proteins show cross-species activity?

    Species cross-reactivity must be investigated individually for each product. Many human cytokines will produce a nice response in mouse cell lines, and many mouse proteins will show activity on human cells. Other proteins may have a lower specific activity when used in the opposite species.

Keywords:

Tetramethylrhodamine-5-iodoacetamide114458-99-05-TMRIAFluorescent DyeInhibitorinhibitorinhibit

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