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Tyramide Amplification Buffer  (Synonyms: 酪氨放大缓冲液)

目录号: HY-D1840
COA 产品使用指南

Tyramide Amplification Buffer 为即用型缓冲液,主要用于通过酪酰胺信号放大 (TSA) 技术对细胞和组织进行的免疫染色。TSA 技术基于酪氨酸酶标记系统,通过使用过氧化物酶将酪氨酸标记的抗体与荧光标记的酪氨酸取代物-酪酰胺 (tyramide) 反应,形成高度放大的荧光信号。例如,辣根过氧化物酶 (HRP) 可在较温和的条件下催化酪氨酸与过氧化氢反应,产生环氧基团。自由基会促进 tyramide与邻近的氨基酸结合,形成荧光标记的产物。

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Tyramide Amplification Buffer Chemical Structure

Tyramide Amplification Buffer Chemical Structure

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     可免费申领三个不同产品的试用装。

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规格 价格 是否有货 数量
2 mL ¥420
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20 mL ¥1540
In-stock
50 mL   询价  
100 mL   询价  

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  • 生物活性

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  • 参考文献

生物活性

Tyramide Amplification Buffer is a ready-to-use buffer primarily intended for immunostaining of cells and tissues by the technique of tyramide signal amplification (TSA). TSA Technology is based on a tyrosinase labeling system that reacts a tyrosine-labeled antibody with a fluorescently labeled tyramide using peroxidase to form a highly amplified fluorescent signal. For example, horseradish peroxidase (HRP) can catalyze the reaction of tyrosine and hydrogen peroxide under milder conditions to generate epoxy groups. Epoxy groups promote the binding of tyramide to adjacent amino acids to form fluorescently labeled products[1].

性状

液体

颜色

Colorless to light yellow

中文名称

酪氨放大缓冲液

运输条件

Room temperature in continental US; may vary elsewhere.

储存方式

-20°C, protect from light

*In solvent : -80°C, 6 months; -20°C, 1 month (protect from light)

纯度 & 产品资料

参考文献

Tyramide Amplification Buffer 相关分类

  • 摩尔计算器

  • 稀释计算器

The molarity calculator equation

Mass (g) = Concentration (mol/L) × Volume (L) × Molecular Weight (g/mol)

质量   浓度   体积   分子量 *
= × ×

The dilution calculator equation

Concentration (start) × Volume (start) = Concentration (final) × Volume (final)

This equation is commonly abbreviated as: C1V1 = C2V2

浓度 (start) × 体积 (start) = 浓度 (final) × 体积 (final)
× = ×
C1   V1   C2   V2
Help & FAQs
  • Do most proteins show cross-species activity?

    Species cross-reactivity must be investigated individually for each product. Many human cytokines will produce a nice response in mouse cell lines, and many mouse proteins will show activity on human cells. Other proteins may have a lower specific activity when used in the opposite species.

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产品名称:
Tyramide Amplification Buffer
目录号:
HY-D1840
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