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  2. Fluorescent Dye
  3. X-Gluc sodium

X-Gluc sodium 是一种检测 β-葡萄糖醛酸酶 (GUS) 的染料试剂 (与 GUS 基因反应,显现蓝色)。β-葡萄糖醛酸酶是一种由大肠杆菌产生的酶,可作为食物、水和泌尿道中大肠杆菌污染的特异性检测指标。X-Gluc sodium 也可广泛地用于分子生物学实验中,标记和检测目的基因的表达 (GUS 报告基因)。

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X-Gluc sodium Chemical Structure

X-Gluc sodium Chemical Structure

CAS No. : 129541-41-9

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规格 价格 是否有货 数量
50 mg ¥1395
In-stock
100 mg ¥2511
In-stock
200 mg   询价  
500 mg   询价  

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Customer Review

Other Forms of X-Gluc sodium:

  • 生物活性

  • 纯度 & 产品资料

  • 参考文献

生物活性

X-Gluc sodium is a dye reagent for the detection of β-glucuronidase (GUS), an enzyme produced by E. coli. X-Gluc sodium can be used to detect E. coli contamination in food, water and the urinary tract (GUS as a specific detection indicator). X-Gluc sodium is also widely used in molecular biology experiments to label and detect the expression of target genes (reacts with the GUS gene, appears blue)[1].

体外研究
(In Vitro)

Guidelines (Following is our recommended protocol. This protocol only provides a guideline, and should be modified according to your specific needs).
1. Dissolve 20 mg X-Gluc sodium in 1mL dimethylformamide (DMF) to prepare X-Gluc master mix.
2. Add the prepared X-Gluc solution to agar medium plates at a final concentration of 50 μg/mL without sterilization.
3. Allow the plates to air dry and be used to inoculate the organisms.
4. Incubate the plates at 35°C and observe 16-24 h after inoculation.

MCE has not independently confirmed the accuracy of these methods. They are for reference only.

分子量

444.59

Formula

C14H12BrClNNaO7

CAS 号
性状

固体

颜色

White to off-white

运输条件

Room temperature in continental US; may vary elsewhere.

储存方式

4°C, sealed storage, away from moisture and light

*In solvent : -80°C, 6 months; -20°C, 1 month (sealed storage, away from moisture and light)

溶解性数据
In Vitro: 

DMSO 中的溶解度 : ≥ 100 mg/mL (224.93 mM; 吸湿的 DMSO 对产品的溶解度有显著影响,请使用新开封的 DMSO)

* "≥" means soluble, but saturation unknown.

配制储备液
浓度 溶剂体积 质量 1 mg 5 mg 10 mg
1 mM 2.2493 mL 11.2463 mL 22.4926 mL
5 mM 0.4499 mL 2.2493 mL 4.4985 mL
查看完整储备液配制表

* 请根据产品在不同溶剂中的溶解度选择合适的溶剂配制储备液;一旦配成溶液,请分装保存,避免反复冻融造成的产品失效
储备液的保存方式和期限:-80°C, 6 months; -20°C, 1 month (sealed storage, away from moisture and light)。-80°C储存时,请在6个月内使用,-20°C储存时,请在1个月内使用。

  • 摩尔计算器

  • 稀释计算器

Mass (g) = Concentration (mol/L) × Volume (L) × Molecular Weight (g/mol)

质量
=
浓度
×
体积
×
分子量 *

Concentration (start) × Volume (start) = Concentration (final) × Volume (final)

This equation is commonly abbreviated as: C1V1 = C2V2

浓度 (start)

C1

×
体积 (start)

V1

=
浓度 (final)

C2

×
体积 (final)

V2

动物溶解方案计算器
请输入动物实验的基本信息:

给药剂量

mg/kg

动物的平均体重

g

每只动物的给药体积

μL

动物数量

由于实验过程有损耗,建议您多配一只动物的量
计算结果
工作液所需浓度 : mg/mL
纯度 & 产品资料

纯度: ≥98.0%

参考文献

X-Gluc sodium 相关分类

完整储备液配制表

* 请根据产品在不同溶剂中的溶解度选择合适的溶剂配制储备液;一旦配成溶液,请分装保存,避免反复冻融造成的产品失效
储备液的保存方式和期限:-80°C, 6 months; -20°C, 1 month (sealed storage, away from moisture and light)。-80°C储存时,请在6个月内使用,-20°C储存时,请在1个月内使用。

可选溶剂 浓度 溶剂体积 质量 1 mg 5 mg 10 mg 25 mg
DMSO 1 mM 2.2493 mL 11.2463 mL 22.4926 mL 56.2316 mL
5 mM 0.4499 mL 2.2493 mL 4.4985 mL 11.2463 mL
10 mM 0.2249 mL 1.1246 mL 2.2493 mL 5.6232 mL
15 mM 0.1500 mL 0.7498 mL 1.4995 mL 3.7488 mL
20 mM 0.1125 mL 0.5623 mL 1.1246 mL 2.8116 mL
25 mM 0.0900 mL 0.4499 mL 0.8997 mL 2.2493 mL
30 mM 0.0750 mL 0.3749 mL 0.7498 mL 1.8744 mL
40 mM 0.0562 mL 0.2812 mL 0.5623 mL 1.4058 mL
50 mM 0.0450 mL 0.2249 mL 0.4499 mL 1.1246 mL
60 mM 0.0375 mL 0.1874 mL 0.3749 mL 0.9372 mL
80 mM 0.0281 mL 0.1406 mL 0.2812 mL 0.7029 mL
100 mM 0.0225 mL 0.1125 mL 0.2249 mL 0.5623 mL
Help & FAQs
  • Do most proteins show cross-species activity?

    Species cross-reactivity must be investigated individually for each product. Many human cytokines will produce a nice response in mouse cell lines, and many mouse proteins will show activity on human cells. Other proteins may have a lower specific activity when used in the opposite species.

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产品名称:
X-Gluc sodium
目录号:
HY-15935C
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