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  4. 5-Carboxyfluorescein diacetate N-succinimidyl ester

5-Carboxyfluorescein diacetate N-succinimidyl ester 

目录号: HY-D0056 纯度: 99.50%
COA 产品使用指南

摩尔计算器

5-Carboxyfluorescein diacetate N-succinimidyl ester 是一种细胞渗透性染料 (Ex=492 nm，Em=517 nm)。5-Carboxyfluorescein diacetate N-succinimidyl ester 可与细胞内分子共价结合，从而标记细胞。5-Carboxyfluorescein diacetate N-succinimidyl ester 可用于示踪淋巴细胞的迁移和增殖。

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5-Carboxyfluorescein diacetate N-succinimidyl ester Chemical Structure

5-Carboxyfluorescein diacetate N-succinimidyl ester Chemical Structure

CAS No. : 150206-05-6

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规格 价格 是否有货 数量
5 mg ￥750
In-stock
10 mg ￥1200
In-stock
25 mg ￥2200
In-stock
50 mg   询价  
100 mg   询价  

* Please select Quantity before adding items.

Customer Review

5-Carboxyfluorescein diacetate N-succinimidyl ester 相关抗体

Top Publications Citing Use of Products

  • 生物活性

  • 纯度 & 产品资料

  • 参考文献

生物活性

5-Carboxyfluorescein diacetate N-succinimidyl ester is a cell permeable dye (Ex=492 nm, Em=517 nm). 5-Carboxyfluorescein diacetate N-succinimidyl ester can label cells by covalently binding to intracellular molecules. 5-Carboxyfluorescein diacetate N-succinimidyl ester is used to track lymphocyte migration and proliferation[1].

体外研究
(In Vitro)

Guidelines (Following is our recommended protocol. This protocol only provides a guideline, and should be modified according to your specific needs)[1].
1. Dilute the stock solution in PBS (to give a 50 μM solution).
2. Add 110 μL of this solution per milliliter of cells (to give a final concentration of 5 μM) and mix rapidly.
3. After 5 min at room temperature, add 10 vol of PBS containing 5% FBS.
4. Centrifuge cells 5 min at 300 ×g, 20 ℃.
5. Remove the supernatant, and wash threetimes, each time by resuspending in 10 vol PBS containing 5% FBS.
6. Centrifuge cells 5 min at 300 × g, 20 ℃, and remove the supernatant.
Note: Labeling occurs rapidly, and it is essential that the tracker is dispersed as evenly and quickly as possible so that cells are uniformly labeled.

MCE has not independently confirmed the accuracy of these methods. They are for reference only.

5-Carboxyfluorescein diacetate N-succinimidyl ester 相关抗体:
分子量

557.46

Formula

C29H19NO11
CAS 号
性状

固体

颜色

White to off-white

Emission (Em)

525
Excitation (Ex)

482
运输条件

Room temperature in continental US; may vary elsewhere.
储存方式

-20°C, protect from light

*In solvent : -80°C, 6 months; -20°C, 1 month (protect from light)

溶解性数据
In Vitro: 

DMSO 中的溶解度 : 20.83 mg/mL (37.37 mM; 超声助溶 (<60°C); 吸湿的 DMSO 对产品的溶解度有显著影响，请使用新开封的 DMSO)

配制储备液
浓度 溶剂体积 质量 1 mg 5 mg 10 mg
1 mM 1.7939 mL 8.9693 mL 17.9385 mL
5 mM 0.3588 mL 1.7939 mL 3.5877 mL
查看完整储备液配制表

* 请根据产品在不同溶剂中的溶解度选择合适的溶剂配制储备液；一旦配成溶液，请分装保存，避免反复冻融造成的产品失效。
储备液的保存方式和期限：-80°C, 6 months; -20°C, 1 month (protect from light)。-80°C储存时，请在6个月内使用，-20°C储存时，请在1个月内使用。

  • 摩尔计算器

  • 稀释计算器

Mass (g) = Concentration (mol/L) × Volume (L) × Molecular Weight (g/mol)

质量
=
浓度
×
体积
×
分子量 *

Concentration (start) × Volume (start) = Concentration (final) × Volume (final)

This equation is commonly abbreviated as: C1V1 = C2V2

浓度 (start)

C1

×
体积 (start)

V1

=
浓度 (final)

C2

×
体积 (final)

V2

动物溶解方案计算器
请输入动物实验的基本信息：

给药剂量

mg/kg

动物的平均体重

g

每只动物的给药体积

μL

动物数量

只
由于实验过程有损耗，建议您多配一只动物的量
计算结果
工作液所需浓度 : mg/mL
纯度 & 产品资料
参考文献

5-Carboxyfluorescein diacetate N-succinimidyl ester 相关分类

完整储备液配制表

* 请根据产品在不同溶剂中的溶解度选择合适的溶剂配制储备液；一旦配成溶液，请分装保存，避免反复冻融造成的产品失效。
储备液的保存方式和期限：-80°C, 6 months; -20°C, 1 month (protect from light)。-80°C储存时，请在6个月内使用，-20°C储存时，请在1个月内使用。

可选溶剂 浓度 溶剂体积 质量 1 mg 5 mg 10 mg 25 mg
DMSO 1 mM 1.7939 mL 8.9693 mL 17.9385 mL 44.8463 mL
5 mM 0.3588 mL 1.7939 mL 3.5877 mL 8.9693 mL
10 mM 0.1794 mL 0.8969 mL 1.7939 mL 4.4846 mL
15 mM 0.1196 mL 0.5980 mL 1.1959 mL 2.9898 mL
20 mM 0.0897 mL 0.4485 mL 0.8969 mL 2.2423 mL
25 mM 0.0718 mL 0.3588 mL 0.7175 mL 1.7939 mL
30 mM 0.0598 mL 0.2990 mL 0.5980 mL 1.4949 mL
Help & FAQs
  • Do most proteins show cross-species activity?

    Species cross-reactivity must be investigated individually for each product. Many human cytokines will produce a nice response in mouse cell lines, and many mouse proteins will show activity on human cells. Other proteins may have a lower specific activity when used in the opposite species.

Keywords:

5-Carboxyfluorescein diacetate N-succinimidyl ester150206-05-6Fluorescent DyeDyelymphocytemigrationproliferationCFSEInhibitorinhibitorinhibit

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5-Carboxyfluorescein diacetate N-succinimidyl ester
目录号：
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