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  4. DAF-FM DA

DAF-FM DA  (Synonyms: Diaminofluorescein-FM diacetate)

目录号: HY-D0717 纯度: ≥98.0%
COA 产品使用指南

摩尔计算器

DAF-FM DA (Diaminofluorescein-FM diacetate) 是一种用于一氧化氮 (NO) 检测和生物成像的荧光探针。 DAF-FM DA 自发穿过质膜，随后被酯酶裂解，生成细胞内 DAF-FM (Ex/Em=495/515 nm)。

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DAF-FM DA Chemical Structure

DAF-FM DA Chemical Structure

CAS No. : 254109-22-3

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规格 价格 是否有货 数量
100μg (5 mM * 40 μL in DMSO) ￥1800
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DAF-FM DA 相关抗体

Top Publications Citing Use of Products

  • 生物活性

  • 纯度 & 产品资料

  • 参考文献

生物活性

DAF-FM DA (Diaminofluorescein-FM diacetate) is a fluorescent probe for the detection and bioimaging of nitric oxide (NO). DAF-FM DA spontaneously crosses the plasma membrane and is subsequently cleaved by esterases to generate intracellular DAF-FM (Ex/Em=495/515 nm)[1].

体外研究
(In Vitro)

BCa 细胞与 5 μM DAF-FM DA 预孵育 30 分钟以加载探针。随后，将细胞与新鲜的 Hank's 缓冲液一起孵育 20 分钟以完成脱酯化。DAF-FM DA 探针可量化细胞中的一氧化氮 (NO) 水平[2]。

MCE has not independently confirmed the accuracy of these methods. They are for reference only.

DAF-FM DA 相关抗体:
体内研究
(In Vivo)

使用经 DAF-FM DA (5 μM) 活染色的斑马鱼胚胎切片，可以确认荧光信号主要位于正在进行的骨形成区域[1]。

MCE has not independently confirmed the accuracy of these methods. They are for reference only.
分子量

496.42

Formula

C25H18F2N2O7
CAS 号
性状

固体

颜色

White to off-white

Emission (Em)

515
Excitation (Ex)

495
运输条件

Room temperature in continental US; may vary elsewhere.
储存方式

-20°C, protect from light

*In solvent : -80°C, 6 months; -20°C, 1 month (protect from light)

纯度 & 产品资料

纯度: ≥98.0%

COA (199 KB)

产品使用指南 (1538 KB)
染色示例
参考文献

DAF-FM DA 相关分类

  • 摩尔计算器

  • 稀释计算器

The molarity calculator equation

Mass (g) = Concentration (mol/L) × Volume (L) × Molecular Weight (g/mol)

质量   浓度   体积   分子量 *
= × ×

The dilution calculator equation

Concentration (start) × Volume (start) = Concentration (final) × Volume (final)

This equation is commonly abbreviated as: C1V1 = C2V2

浓度 (start) × 体积 (start) = 浓度 (final) × 体积 (final)
× = ×
C1   V1   C2   V2
Help & FAQs
  • Do most proteins show cross-species activity?

    Species cross-reactivity must be investigated individually for each product. Many human cytokines will produce a nice response in mouse cell lines, and many mouse proteins will show activity on human cells. Other proteins may have a lower specific activity when used in the opposite species.

Keywords:

DAF-FM DA254109-22-3Diaminofluorescein-FM diacetateFluorescent DyeNOInhibitorinhibitorinhibit

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