2. Immunology/Inflammation NF-κB Metabolic Enzyme/Protease
  3. Reactive Oxygen Species (ROS)
  4. H2DCFDA

H2DCFDA  (Synonyms: DCFH-DA; 2',7'-Dichlorodihydrofluorescein diacetate)

目录号: HY-D0940 纯度: 99.81%
COA 产品使用指南 技术支持

H2DCFDA (DCFH-DA) 是一种可渗透细胞的，用于检测细胞内活性氧 (ROS) 的探针 (Ex/Em=488/525 nm)。

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CAS No. : 4091-99-0

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规格 价格 是否有货 数量
5 mg ￥156
In-stock
10 mg ￥250
In-stock
25 mg ￥370
In-stock
50 mg ￥500
In-stock
100 mg ￥700
In-stock
250 mg ￥1400
In-stock
500 mg ￥2100
In-stock
1 g ￥3150
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H2DCFDA 相关抗体

Top Publications Citing Use of Products

MCE 顾客使用本产品发表的 496 篇科研文献

IF

    H2DCFDA purchased from MCE. Usage Cited in: Adv Mater. 2025 Jan 10:e2415030.  [Abstract]

    DCFH-DA (5 μM; 30 min). DCFH‐DA, a specific indicator for ROS detection, is employed to evaluate overall ROS levels in 4T1 cells.

    H2DCFDA purchased from MCE. Usage Cited in: ACS Nano. 2025 Jan 25.  [Abstract]

    Inside A375 melanoma cells, the ROS level is measured with the help of the DCFH-DA probe under CLSM imaging.
    Representative CLSM images of ROS generation inside A375 cells after different treatments with or without light irradiation (scale bar: 50 μm; blue: Hoechst 33342, green: DCFH-DA probe; Xe lamp, 650 nm, 6.4 mW/cm2, 12 min).

    H2DCFDA purchased from MCE. Usage Cited in: ACS Nano. 2025 Jan 24.  [Abstract]

    DCFH-DA (10 μM; 30 min). Fluorescent images of ROS levels in PC12 cells. ROS were labeled with the DCFH-DA probe (green) and nuclei were stained with Hoechst 33342 (blue). Scale bar, 40 μm. Data are presented as mean ± SD.

    H2DCFDA purchased from MCE. Usage Cited in: ACS Nano. 2025 Jan 24.  [Abstract]

    DCFH-DA (10 μM; 30 min). (B) Flow cytometry analysis of ROS levels in HQMitofactories using the DCFH-DA probe. HQ-Mitofactories were exposed to 400 μM H2O2 for 6 h prior to measurement (n = 3; **** is P <0.0001 by one-way ANOVA test). (C) Confocal fluorescent images of ROS levels in HQ-Mitofactories. ROS were labeled with the DCFH-DA probe (green) and nuclei were stained with Hoechst 33342 (blue). Scale bar, 50 μm.

    H2DCFDA purchased from MCE. Usage Cited in: Adv Mater. 2024 Oct 12:e2411618.  [Abstract]

    DCFH-DA (30 min). The capability of different biocatalysts to eliminate ROS was assessed through c) representative images and d) quantitative analysis (n = 3 independent biological replicates) of DCFH-DA fluorescence across various cell types (MSCs, RSC96, Raw264.7) subjected to distinct treatments.

    H2DCFDA purchased from MCE. Usage Cited in: Adv Mater. 2024 Nov 26:e2409176.  [Abstract]

    DCFH-DA (10 µm; 20 min). The ROS levels in the oxygen-glucose deprivation/reoxygenation (OGD/R) model of HT22 cells were detected using DCFH-DA. Confocal laser scanning microscope (CLSM) images showed bright green fluorescence in the OGD/R group, indicating a significant increase in ROS.

    H2DCFDA purchased from MCE. Usage Cited in: Bioact Mater. 2024 Jul 9:41:30-45.  [Abstract]

    DCFH-DA (20 min). DCFH-DA assay is further examined to evaluate the ROS scavenging capacity on the cellular level. As ROS oxidizes DCFH into DCF, it emits green fluorescence, which can be used to estimate the remaining ROS.

    H2DCFDA purchased from MCE. Usage Cited in: Adv Funct Mater. 2024 May 23.

    DCFH-DA (10 µM; 20 min). Fluorescence images of DCFH illustrating intracellular ROS in BMSCs after different treatment (scale bar: 200 µm).

    H2DCFDA purchased from MCE. Usage Cited in: J Extracell Vesicles. 2024 Sep;13(9):e12505.  [Abstract]

    H2DCFDA (10 µM; 20 min). Detection of intracellular ROS generation in A549 cells exposed to 20 µM H2O2. Scale bar: 200 µm. Right, quantification of corresponding fluorescence intensity.

    H2DCFDA purchased from MCE. Usage Cited in: Acta Pharm Sin B. 16 July 2022.

    Treated HL-1 cells are washed three times and incubated with DMEM medium containing H2DCFDA (10 μM; 30 min) at 37 °C. The cells then are washed three times with warm DMEM medium and intracellular ROS production is detected by flow cytometry.

    H2DCFDA purchased from MCE. Usage Cited in: Adv Sci (Weinh). 2022 Mar 10;e2103317.  [Abstract]

    CLSM images of HUVEC cells using DCFH‐DA (0.5 hours) as the fluorescent probe: Control, H2O2, H2O2+10 µg/mL1 PDA NPs, H2O2+40 µg/mL PDA NPs, H2O2+80 µg/mL PDA NPs.

    H2DCFDA purchased from MCE. Usage Cited in: Biomaterials. 2022 Sep 30;290:121842.  [Abstract]

    DCFH-DA (10 μM; 30 min) or C11-BODIPY-labeled cells are substantially increased after treatment with 1 μM FeSO4.

    H2DCFDA purchased from MCE. Usage Cited in: Biomaterials. 2022 May;284:121513.  [Abstract]

    DCFH-DA (10 μM; 30 min). The ROS generation in 4T1 cells and NIH-3T3 cells incubated with various formulations for 4 h observed by CLSM.

    H2DCFDA purchased from MCE. Usage Cited in: Biomaterials. 2022 Jul 21;287:121688.  [Abstract]

    CLSM images of 4T1 tumor cells stained with DCFH-DA (15 min) after different treatments.

    H2DCFDA purchased from MCE. Usage Cited in: J Hazard Mater. 2022 Oct 5;439:129502.  [Abstract]

    Representative DCFH-DA (10 μM; 30 min) staining images (Green) in PBNs exposed PSNPs and/or LPS in vivo and in vitro.

    H2DCFDA purchased from MCE. Usage Cited in: Cell. 2021 Jun 24;184(13):3528-3541.e12.  [Abstract]

    Stained protoplasts with H2DCFDA for the detection of ROS production and PI for PM integrity and tracked the process by live-cell imaging.

    H2DCFDA purchased from MCE. Usage Cited in: ACS Nano. 2021 Oct 26;15(10):16286-16297.  [Abstract]

    CLSM image of 4T1 cells after being treated with MM, MMP, and MMTP; cells are stained with DCFH-DA (20 μM; for 1 h).

    H2DCFDA purchased from MCE. Usage Cited in: Biomaterials. 2021 Oct;277:121103.  [Abstract]

    In vitro experiments are conducted to measure the cellular ROS levels in A549 cells from different treatments via flow cytometry using fluorescent probe H2DCFDA (5 μM; 30 min) to better understand the potential molecular mechanism of SOD@ARA290-HBc on radiation-induced lung injury. H2DCFDA fluorescence intensity in A549 cells significantly increased after irradiation.

    H2DCFDA purchased from MCE. Usage Cited in: Small. 2021 Aug;17(32):e2101368.  [Abstract]

    Laser scanning confocal fluorescence microscopy images of HT-1080 cells treated with different nanodrugs for the detection of intracellular lipid peroxidation and total ROS using C11-BODIPY and DCFH-DA (100 nM; 10 min) as the corresponding fluorescence probes.

    H2DCFDA purchased from MCE. Usage Cited in: Curr Biol. 2021 May 18;S0960-9822(21)00607-2.  [Abstract]

    Co-staining stigmas with H2DCFDA (50 μM; for 1-2 hours) and the cell wall indicator propidium iodide (PI) shows that ROS is located in the cytoplasm of papilla cells, near the periphery of the plasma membrane.

    • 生物活性

    • 实验参考方法

    • 纯度 & 产品资料

    • 参考文献

    生物活性

    H2DCFDA (DCFH-DA) is a cell-permeable probe used to detect intracellular reactive oxygen species (ROS) (Ex/Em=488/525 nm)[1].

    体外研究
    (In Vitro)

    指南 (以下是我们推荐的方案，本方案仅提供指南，应根据您的具体需要进行修改)。
    1.1 制备储存液
    用 无水 DMSO 配制 10 mM 的 H2DCFDA 储备液。
    注：H2DCFDA 储存液建议分装后于 -20 ℃ 或 -80 ℃ 避光保存。
    1.2 工作液的配制
    用预热好的无血清细胞培养基或 PBS 稀释储存液，配制成 1-10 μM 的 H2DCFDA工作液。
    注：请根据实际情况调整 H2DCFDA 工作液浓度，且现用现配。
    2. 细胞染色（悬浮细胞）
    2.1 离心收集细胞，加入 PBS 洗涤两次，每次 5 分钟。细胞密度在 1×106/mL。
    2.2 加入 1 mL 染料 工作液，室温孵育 5-30 分钟。
    2.3 400 g，离心 3-4 分钟，弃去上清。
    2.4 加入 PBS 洗涤细胞两次，每次 5 分钟。
    2.5 用 1 mL 无血清培养基或 PBS 重悬细胞后，使用荧光显微镜或流式细胞仪进行观察。
    3. 细胞染色（贴壁细胞）
    3.1 将贴壁细胞培养于无菌盖玻片上。
    3.2 从培养基中移出盖玻片，吸除多余培养基。
    3.3 加入 100 μL 染料工作液，轻轻晃动使其完全覆盖细胞，孵育 5-30 分钟。
    3.4 吸去染料工作液，用培养基洗 2-3 次，每次 5 分钟 ，使用荧光显微镜或流式细胞仪进行 观察。
    注：若需要用流式细胞仪检测，需将细胞用胰蛋白酶消化重悬后再进行染色。

    MCE has not independently confirmed the accuracy of these methods. They are for reference only.

    H2DCFDA 相关抗体:
    分子量

    487.29

    Formula

    C24H16Cl2O7
    CAS 号
    性状

    固体

    颜色

    White to off-white

    Emission (Em)

    517
    Excitation (Ex)

    492
    运输条件

    Room temperature in continental US; may vary elsewhere.
    储存方式

    -20°C, protect from light

    *In solvent : -80°C, 6 months; -20°C, 1 month (protect from light)

    溶解性数据
    细胞实验： 

    DMSO 中的溶解度 : 250 mg/mL (513.04 mM; 超声助溶; 吸湿的 DMSO 对产品的溶解度有显著影响，请使用新开封的 DMSO)

    配制储备液
    浓度 溶剂体积 质量 1 mg 5 mg 10 mg
    1 mM 2.0522 mL 10.2608 mL 20.5217 mL
    5 mM 0.4104 mL 2.0522 mL 4.1043 mL
    查看完整储备液配制表

    * 请根据产品在不同溶剂中的溶解度选择合适的溶剂配制储备液；一旦配成溶液，请分装保存，避免反复冻融造成的产品失效。
    储备液的保存方式和期限：-80°C, 6 months; -20°C, 1 month (protect from light)。-80°C储存时，请在6个月内使用，-20°C储存时，请在1个月内使用。

    • 摩尔计算器

    • 稀释计算器

    Mass (g) = Concentration (mol/L) × Volume (L) × Molecular Weight (g/mol)

    质量
    =
    浓度
    ×
    体积
    ×
    分子量 *

    Concentration (start) × Volume (start) = Concentration (final) × Volume (final)

    This equation is commonly abbreviated as: C1V1 = C2V2

    浓度 (start)

    C1

    ×
    体积 (start)

    V1

    =
    浓度 (final)

    C2

    ×
    体积 (final)

    V2

    动物溶解方案计算器
    请输入动物实验的基本信息：

    给药剂量

    mg/kg

    动物的平均体重

    g

    每只动物的给药体积

    μL

    动物数量

    只
    由于实验过程有损耗，建议您多配一只动物的量
    计算结果
    工作液所需浓度 : mg/mL
    纯度 & 产品资料

    纯度: 99.82%

    COA (289 KB) HNMR (228 KB) LCMS (120 KB)

    产品使用指南 (1538 KB)
    染色示例
    参考文献
    Kinase Assay

    ROS Measurements[1]
    For the detection of intracellular ROS level ,ROS-sensitive probe H2DCFDA is used. Adherent cells (ESCs, difESCs, eMSCs, HeLa, U118) are incubated with 5 µM staining solution in PBS in the dark for 30 min at 37°C, then harvested with 0.05% trypsin-EDTA solution, suspended in a fresh medium, and immediately analyzed with flow cytometer. Lymphocytes, both control and PHA-activated, are resuspended in PBS, incubated with 5 µM of H2DCFDA in the dark for 30 min at 37°C, and immediately analyzed. Along with the H2DCFDA probe, if indicated, ROS-insensitive modification of the fluorescent dye DCFDA is used. The staining procedure is the same as for the H2DCFDA[1].

    MCE has not independently confirmed the accuracy of these methods. They are for reference only.
    参考文献

    H2DCFDA 相关分类

    完整储备液配制表

    * 请根据产品在不同溶剂中的溶解度选择合适的溶剂配制储备液；一旦配成溶液，请分装保存，避免反复冻融造成的产品失效。
    储备液的保存方式和期限：-80°C, 6 months; -20°C, 1 month (protect from light)。-80°C储存时，请在6个月内使用，-20°C储存时，请在1个月内使用。

    可选溶剂 浓度 溶剂体积 质量 1 mg 5 mg 10 mg 25 mg
    DMSO 1 mM 2.0522 mL 10.2608 mL 20.5217 mL 51.3042 mL
    5 mM 0.4104 mL 2.0522 mL 4.1043 mL 10.2608 mL
    10 mM 0.2052 mL 1.0261 mL 2.0522 mL 5.1304 mL
    15 mM 0.1368 mL 0.6841 mL 1.3681 mL 3.4203 mL
    20 mM 0.1026 mL 0.5130 mL 1.0261 mL 2.5652 mL
    25 mM 0.0821 mL 0.4104 mL 0.8209 mL 2.0522 mL
    30 mM 0.0684 mL 0.3420 mL 0.6841 mL 1.7101 mL
    40 mM 0.0513 mL 0.2565 mL 0.5130 mL 1.2826 mL
    50 mM 0.0410 mL 0.2052 mL 0.4104 mL 1.0261 mL
    60 mM 0.0342 mL 0.1710 mL 0.3420 mL 0.8551 mL
    80 mM 0.0257 mL 0.1283 mL 0.2565 mL 0.6413 mL
    100 mM 0.0205 mL 0.1026 mL 0.2052 mL 0.5130 mL
    Help & FAQs
    • Do most proteins show cross-species activity?

      Species cross-reactivity must be investigated individually for each product. Many human cytokines will produce a nice response in mouse cell lines, and many mouse proteins will show activity on human cells. Other proteins may have a lower specific activity when used in the opposite species.

    Keywords:

    H2DCFDA4091-99-0DCFH-DA 2',7'-Dichlorodihydrofluorescein diacetateReactive Oxygen Species (ROS)Inhibitorinhibitorinhibit

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