PrP (106-126) (Synonyms: Prion protein (106-126))

目录号: HY-P0305 纯度: 98.78%: FAQs
Data Sheet SDS COA 产品使用指南

摩尔计算器

PrP (106-126) 是与朊病毒蛋白的淀粉样区域相对应的多肽段，它的生化特性类似于朊病毒蛋白的感染区域。

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Custom Peptide Synthesis

PrP (106-126) Chemical Structure

CAS No. : 148439-49-0

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规格	价格	是否有货
1 mg	￥800	In-stock
5 mg	￥2000	In-stock
10 mg	￥3000	In-stock
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100 mg		询价

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生物活性
实验参考方法
纯度 & 产品资料
参考文献

生物活性

PrP (106-126) is a peptide corresponding to the prion protein (PrP) amyloidogenic region, and its biochemical properties resemble the infectious form of prion protein.

体外研究
(In Vitro)

PrP (106-126) (100 μM) induces mTOR phosphorylation over time in N2a cells. PrP (106-126)-treated cells show significantly increased ROS production in comparison with that of PBS-treated control cells. Knockdown of PRAS40 enhances PrP (106-126)-induced apoptosis. PRAS40 alleviates PrP (106-126)-induced neuronal apoptosis via mTOR-AKT activation^[1]. PrP (106-126) interacts selectively with porcine brain endothelial cells (PBEC) via their luminal side, and causes cumulative cell death, as shown by lactate dehydrogenase release, 3-(4,5-dimethylthiazol-2-yl)-2,5-diphenyltetrazolium bromide reduction, Caspase 3 induction and direct cell counting. In addition, PrP (106-126), but not its corresponding scrambled peptide, produces a 50% reduction of the trans-endothelial electrical resistance, while the PBEC maintained confluency^[2].

MCE has not independently confirmed the accuracy of these methods. They are for reference only.

分子量

1912.30

Formula

C₈₀H₁₃₈N₂₆O₂₄S₂

CAS 号

148439-49-0

性状

固体

颜色

White to off-white

Sequence

Lys-Thr-Asn-Met-Lys-His-Met-Ala-Gly-Ala-Ala-Ala-Ala-Gly-Ala-Val-Val-Gly-Gly-Leu-Gly

Sequence Shortening

KTNMKHMAGAAAAGAVVGGLG

运输条件

Room temperature in continental US; may vary elsewhere.

储存方式

Sealed storage, away from moisture

Powder	-80°C	2 years
	-20°C	1 year

*In solvent : -80°C, 6 months; -20°C, 1 month (sealed storage, away from moisture)

溶解性数据

In Vitro:

H₂O 中的溶解度 : 1 mg/mL (0.52 mM; 超声加热助溶)

摩尔计算器
稀释计算器

Mass (g) = Concentration (mol/L) × Volume (L) × Molecular Weight (g/mol)

Concentration _(start) × Volume _(start) = Concentration _(final) × Volume _(final)

This equation is commonly abbreviated as: C₁V₁ = C₂V₂

浓度 _(start)

体积 _(start)

浓度 _(final)

体积 _(final)

In Vivo:

以下溶解方案，请直接配置工作液。建议现用现配，在短期内尽快用完。以下溶剂前显示的百分比是指该溶剂在您配制终溶液中的体积占比；如在配制过程中出现沉淀、析出现象，可以通过加热和/或超声的方式助溶。

方案一

请依序添加每种溶剂： PBS
Solubility: 100 mg/mL (52.29 mM); 澄清溶液; 超声助溶

动物溶解方案计算器

请输入动物实验的基本信息：

给药剂量

mg/kg

动物的平均体重

每只动物的给药体积

μL

动物数量

只

由于实验过程有损耗，建议您多配一只动物的量

计算结果

工作液所需浓度 : mg/mL

您所需的储备液浓度超过该产品的实测溶解度，如有需要，请与 MCE 中国技术支持联系。

纯度 & 产品资料

纯度: 98.78%

选择批次：

Data Sheet (605 KB) SDS (252 KB)

COA (257 KB) RP-HPLC (252 KB)

产品使用指南 (1538 KB)

参考文献

[1]. Yang W, et al. PRAS40 alleviates neurotoxic prion peptide-induced apoptosis via mTOR-AKT signaling. CNS Neurosci Ther. 2017 May;23(5):416-427. [Content Brief]

[2]. Cooper I, et al. Interactions of the prion peptide (PrP 106-126) with brain capillary endothelial cells: coordinated cell killing and remodeling of intercellular junctions. J Neurochem. 2011 Feb;116(4):467-75. [Content Brief]

Cell Assay ^[2]	The toxicity of PrP peptides is investigated on monolayers of PBEC. 100 000 PBEC/cm² are seeded on 96 [for 3-(4,5-dimethylthiazol-2-yl)-2,5-diphenyltetrazolium bromide (MTT)] or 24-well plates [for lactate dehydrogenase (LDH)] pre-coated with rat tail collagen (27 μg/mL). The cell monolayers are treated with either PrP 106-126 wt or scr peptides. The PrP peptides are dissolved in double distilled water and then diluted in assay medium or in 5% newborn calf serum in Dulbecco-modified Earl's medium without phenol red. Treatment duration with the PrP peptides varies from 24 h to 48 h and the peptide concentration is set to 100 μM at which it exerts optimal effects as shown in a dose-dependent experiment. This concentration is also chosen as similar concentrations of PrP 106-126 found to exert in-vitro effects on astrocytes, neurons, microglia and leukocytes. The MTT assay, which measures mitochondrial metabolism, is based on the conversion of the water-soluble MTT dye [3-(4,5-dimethylthiazol-2-yl)-2,5-diphenyltetrazolium bromide] to an insoluble purple formazan. This formazan is then solubilized in pure dimethylsulfoxide, and its concentration determined by optical density (550/630 nm). The LDH cell death assay is done using the CytoTox 96® Assay kit. The released LDH in culture supernatants is measured with a 30-min coupled enzymatic assay which results in the conversion of a tetrazolium salt, 2-p-iodophenyl-3-p-nitro-phenyl-5-phenyl tetrazolium chloride (INT) into a red formazan product. The LDH levels are measured at the end of each treatment period. Thus, 50 μL of the medium is removed at the end of the treatment with PrP peptides and transferred to a new 96-well plate to which the other compounds are added. The plates are analyzed using an Elisa reader with the 490 nm filter. MCE has not independently confirmed the accuracy of these methods. They are for reference only.
参考文献	[1]. Yang W, et al. PRAS40 alleviates neurotoxic prion peptide-induced apoptosis via mTOR-AKT signaling. CNS Neurosci Ther. 2017 May;23(5):416-427. [Content Brief] [2]. Cooper I, et al. Interactions of the prion peptide (PrP 106-126) with brain capillary endothelial cells: coordinated cell killing and remodeling of intercellular junctions. J Neurochem. 2011 Feb;116(4):467-75. [Content Brief]

Cell Assay
^[2]

The toxicity of PrP peptides is investigated on monolayers of PBEC. 100 000 PBEC/cm² are seeded on 96 [for 3-(4,5-dimethylthiazol-2-yl)-2,5-diphenyltetrazolium bromide (MTT)] or 24-well plates [for lactate dehydrogenase (LDH)] pre-coated with rat tail collagen (27 μg/mL). The cell monolayers are treated with either PrP 106-126 wt or scr peptides. The PrP peptides are dissolved in double distilled water and then diluted in assay medium or in 5% newborn calf serum in Dulbecco-modified Earl's medium without phenol red. Treatment duration with the PrP peptides varies from 24 h to 48 h and the peptide concentration is set to 100 μM at which it exerts optimal effects as shown in a dose-dependent experiment. This concentration is also chosen as similar concentrations of PrP 106-126 found to exert in-vitro effects on astrocytes, neurons, microglia and leukocytes. The MTT assay, which measures mitochondrial metabolism, is based on the conversion of the water-soluble MTT dye [3-(4,5-dimethylthiazol-2-yl)-2,5-diphenyltetrazolium bromide] to an insoluble purple formazan. This formazan is then solubilized in pure dimethylsulfoxide, and its concentration determined by optical density (550/630 nm). The LDH cell death assay is done using the CytoTox 96® Assay kit. The released LDH in culture supernatants is measured with a 30-min coupled enzymatic assay which results in the conversion of a tetrazolium salt, 2-p-iodophenyl-3-p-nitro-phenyl-5-phenyl tetrazolium chloride (INT) into a red formazan product. The LDH levels are measured at the end of each treatment period. Thus, 50 μL of the medium is removed at the end of the treatment with PrP peptides and transferred to a new 96-well plate to which the other compounds are added. The plates are analyzed using an Elisa reader with the 490 nm filter.

MCE has not independently confirmed the accuracy of these methods. They are for reference only.

参考文献

[1]. Yang W, et al. PRAS40 alleviates neurotoxic prion peptide-induced apoptosis via mTOR-AKT signaling. CNS Neurosci Ther. 2017 May;23(5):416-427. [Content Brief]

[2]. Cooper I, et al. Interactions of the prion peptide (PrP 106-126) with brain capillary endothelial cells: coordinated cell killing and remodeling of intercellular junctions. J Neurochem. 2011 Feb;116(4):467-75. [Content Brief]

Help & FAQs

Do most proteins show cross-species activity?
Species cross-reactivity must be investigated individually for each product. Many human cytokines will produce a nice response in mouse cell lines, and many mouse proteins will show activity on human cells. Other proteins may have a lower specific activity when used in the opposite species.

Keywords:

PrP (106-126)148439-49-0Prion protein (106-126)OthersInhibitorinhibitorinhibit

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PrP (106-126) (Synonyms: Prion protein (106-126))

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PrP (106-126) 相关产品

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实验参考方法

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参考文献

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PrP (106-126) (Synonyms: Prion protein (106-126))

Customer Review

PrP (106-126) 相关抗体

PrP (106-126) 相关产品

•相关化合物库:

•同靶点产品:

•同靶点蛋白产品:

生物活性

实验参考方法

纯度 & 产品资料

参考文献

PrP (106-126) 相关抗体:

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稀释计算器

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