1. Apoptosis
  2. Caspase
  3. Ac-LEHD-AFC

Ac-LEHD-AFC 是 caspase-9 的荧光底物,可用于检测 caspase 的活性。

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Ac-LEHD-AFC Chemical Structure

Ac-LEHD-AFC Chemical Structure

CAS No. : 210345-03-2

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1 mg ¥500
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5 mg ¥1300
In-stock
10 mg ¥2100
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100 mg   询价  

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MCE 顾客使用本产品发表的 1 篇科研文献

  • 生物活性

  • 纯度 & 产品资料

  • 参考文献

生物活性

Ac-LEHD-AFC is a fluorescent substrate for caspase-9, can be used to assess the activity of caspase[1].

IC50 & Target

Caspase-9[1]

体外研究
(In Vitro)

Guidelines (Following is our recommended protocol. This protocol only provides a guideline, and should be modified according to your specific needs).
The assessment of the caspase activity by fluorogenic substrate assay[1]:
1. Preparation of cell lysis buffer: 1 % Nonidet P-40, NaCl 200 mM, Tris/HCl 20 mM, pH 7.4, Leupeptin 10 mg/L, Aprotinin (HY-P0017) (0.27 kU/L) and 100 mM PMSF (HY-B0496) .
2. Incubate the lysis buffer (25 mg of total protein) with 50 mmol/L of AC-DEVD-AMC (HY-P1003) (caspase-3), AC-VEID-AFC (caspase-8) or AC-LEHD-AFC (caspase-9) in the buffer (HEPES 10 mM, pH 7.4, containing Mannitol 220 mM, Sucrose 68 mM, NaCl 2 mM, KH2PO4 2.5 mM, EGTA 0.5 mM, MgCl2 2 mM, Pyruvate 5 mM, PMSF 0.1 mM, and Dithiothreitol 1 mM).
3. Assess the caspase activity by measuring fluorescent 7-amino-4-methylcoumarin released for 1 h at a 2-min intervals by a spectrofluorometer.

MCE has not independently confirmed the accuracy of these methods. They are for reference only.

分子量

765.69

Formula

C33H38F3N7O11

CAS 号
性状

固体

颜色

White to off-white

Emission (Em)

454

Excitation (Ex)

445

运输条件

Room temperature in continental US; may vary elsewhere.

储存方式

-20°C, sealed storage, away from moisture and light

*In solvent : -80°C, 6 months; -20°C, 1 month (sealed storage, away from moisture and light)

溶解性数据
细胞实验: 

DMSO 中的溶解度 : ≥ 100 mg/mL (130.60 mM; 吸湿的 DMSO 对产品的溶解度有显著影响,请使用新开封的 DMSO)

* "≥" means soluble, but saturation unknown.

配制储备液
浓度 溶剂体积 质量 1 mg 5 mg 10 mg
1 mM 1.3060 mL 6.5301 mL 13.0601 mL
5 mM 0.2612 mL 1.3060 mL 2.6120 mL
查看完整储备液配制表

* 请根据产品在不同溶剂中的溶解度选择合适的溶剂配制储备液;一旦配成溶液,请分装保存,避免反复冻融造成的产品失效
储备液的保存方式和期限:-80°C, 6 months; -20°C, 1 month (sealed storage, away from moisture and light)。-80°C储存时,请在6个月内使用,-20°C储存时,请在1个月内使用。

  • 摩尔计算器

  • 稀释计算器

Mass (g) = Concentration (mol/L) × Volume (L) × Molecular Weight (g/mol)

质量
=
浓度
×
体积
×
分子量 *

Concentration (start) × Volume (start) = Concentration (final) × Volume (final)

This equation is commonly abbreviated as: C1V1 = C2V2

浓度 (start)

C1

×
体积 (start)

V1

=
浓度 (final)

C2

×
体积 (final)

V2

动物溶解方案计算器
请输入动物实验的基本信息:

给药剂量

mg/kg

动物的平均体重

g

每只动物的给药体积

μL

动物数量

由于实验过程有损耗,建议您多配一只动物的量
计算结果
工作液所需浓度 : mg/mL
纯度 & 产品资料

纯度: 98.22%

参考文献

完整储备液配制表

* 请根据产品在不同溶剂中的溶解度选择合适的溶剂配制储备液;一旦配成溶液,请分装保存,避免反复冻融造成的产品失效
储备液的保存方式和期限:-80°C, 6 months; -20°C, 1 month (sealed storage, away from moisture and light)。-80°C储存时,请在6个月内使用,-20°C储存时,请在1个月内使用。

可选溶剂 浓度 溶剂体积 质量 1 mg 5 mg 10 mg 25 mg
DMSO 1 mM 1.3060 mL 6.5301 mL 13.0601 mL 32.6503 mL
5 mM 0.2612 mL 1.3060 mL 2.6120 mL 6.5301 mL
10 mM 0.1306 mL 0.6530 mL 1.3060 mL 3.2650 mL
15 mM 0.0871 mL 0.4353 mL 0.8707 mL 2.1767 mL
20 mM 0.0653 mL 0.3265 mL 0.6530 mL 1.6325 mL
25 mM 0.0522 mL 0.2612 mL 0.5224 mL 1.3060 mL
30 mM 0.0435 mL 0.2177 mL 0.4353 mL 1.0883 mL
40 mM 0.0327 mL 0.1633 mL 0.3265 mL 0.8163 mL
50 mM 0.0261 mL 0.1306 mL 0.2612 mL 0.6530 mL
60 mM 0.0218 mL 0.1088 mL 0.2177 mL 0.5442 mL
80 mM 0.0163 mL 0.0816 mL 0.1633 mL 0.4081 mL
100 mM 0.0131 mL 0.0653 mL 0.1306 mL 0.3265 mL
Help & FAQs
  • Do most proteins show cross-species activity?

    Species cross-reactivity must be investigated individually for each product. Many human cytokines will produce a nice response in mouse cell lines, and many mouse proteins will show activity on human cells. Other proteins may have a lower specific activity when used in the opposite species.

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Inquiry Information

产品名称:
Ac-LEHD-AFC
目录号:
HY-P1093
需求量: