Acetyl-Histone H3 (Lys4) 抗体

Acetyl-Histone H3 (Lys4) Antibody is a non-conjugated and Rabbit origined monoclonal antibody about 15 kDa, targeting to Acetyl-Histone H3 (Lys4). It can be used for WB,ICC/IF assays with tag free, in the background of Human, Mouse.

Acetyl-Histone H3 (Lys4)：组蛋白是基本的核蛋白，负责真核生物染色体纤维的核小体结构。这种结构由大约 146 bp 的 DNA 组成，核小体是一个八聚体，由四种核心组蛋白（H2A、H2B、H3 和 H4）中的每一种成对组成。染色质纤维通过接头组蛋白 H1 与核小体之间的 DNA 相互作用进一步压缩，形成更高阶的染色质结构。该基因是无内含子的，编码复制依赖组蛋白，该组蛋白是组蛋白 H3 家族的成员。该基因的转录本缺少 polyA 尾巴；相反，它们包含回文终止元素。该基因存在于染色体 6p22-p21.3 上的大组蛋白基因簇中。[由 RefSeq 提供，2015 年 8 月]

Free

8350

Predicted band size: 15 kDa

affinity purified

Non-conjugated

Epigenetics and Nuclear Signaling

Primary Antibody; Recombinant Rabbit Monoclonal Antibody

Rabbit

Human, Mouse

P68431

WB: 1:500-1:1,000 ;IF: 1:50-1:200

• 
  Western blot analysis of extracts from Hela(lane 2(20μg) and Hela (lane 3(20μg)), Trichostatin A treated (HY-15144, 1μM, 18hrs) as indicated, using Acetyl-Histone H3 (Lys4) (HY-P80531) Rabbit mAb. Proteins were transferred to a PVDF membrane and blocked with 5% BSA in TBST for 2 hour at room temperature. The primary antibody (HY-P80531, 1/1000) and Loading control antibody (GAPDH, HY-P80954, 1/3000) was used in 5% BSA in TBST at 4°C overnight. Goat Anti-Mouse/Rabbit IgG-HRP Secondary Antibody (HY-P8004/HY-P8001, 1/10,000) was used for 1 hour at room temperature.

WB, ICC/IF

液体

Supplied in 50 mM Tris-Glycine (pH 7.4), 0.15 M NaCl, 40% Glycerol and 0.05% BSA. Preservative: 0.01% Sodium azide

Stored at -20°C for 1 year. Avoid repeated freeze / thaw cycles.

Shipping with blue ice.

IgG

Synthetic peptide corresponding to residues surrounding Lys4 of Human Human Histone H3.The exact sequence is proprietary to MCE.