Alexa Fluor® 647-conjugated AffiniPure Goat Anti-Mouse IgG H&L

目录号: HY-P81013: Data Sheet SDS COA 抗体使用指南
FAQs
技术支持

Alexa Fluor® 647-conjugated AffiniPure Goat Anti-Mouse IgG H&L 是偶联了 Alexa Fluor® 647 标记的、山羊来源的抗小鼠 IgG 抗体。Alexa Fluor® 647-conjugated AffiniPure Goat Anti-Mouse IgG H&L 可结合小鼠 IgG 抗体的轻链和重链。

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规格	价格	是否有货
50 μL	￥200	In-stock
100 μL	￥300	In-stock
250 μL		询价

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Alexa Fluor® 647-conjugated AffiniPure Goat Anti-Mouse IgG H&L 相关产品:

WB: 蛋白质免疫印迹;
IHC-P: 石蜡切片样本的免疫组织化学;
IHC-F: 冰冻切片样本的免疫组织化学;
ICC/IF: 细胞免疫荧光;
IF-Tissue: 组织免疫荧光;
mIHC: 多重荧光免疫组化;
IP: 免疫沉淀;
ChIP: 染色质免疫沉淀;
FC: 流式细胞术;
ELISA: 酶联免疫吸附试验

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描述

Alexa Fluor® 647-conjugated AffiniPure Goat Anti-Mouse IgG H&Lis an -conjugated, goat-derived anti-mouse IgG antibody. Alexa Fluor® 647-conjugated AffiniPure Goat Anti-Mouse IgG H&L conjugates the light and heavy chains of mouse IgG antibodies.

宿主

Goat

克隆性

Polyclonal

反应种属

Mouse

应用 & 推荐
稀释比例

应用	稀释比
ICC/IF ICC/IF: 细胞免疫荧光	1:500-1:1000
IHC-F IHC-F: 冰冻切片样本的免疫组织化学	1:100-1:1000
FC FC: 流式细胞术	1:500-1:1000
ELISA ELISA: 酶联免疫吸附试验	1:5000-1:20000

敏感性	Endogenous	偶联	Alexa Fluor® 647
修饰	Unmodified	同型	IgG

性状

液体

组分

Supplied in 0.01M Phosphate Buffered Saline, pH 7.2, containing 1% BSA, 50% glycerol, 0.02% Sodium Azide.

保存条件 & 期限

Stored at -20°C for 1 year.Protect from light. Avoid repeated freeze / thaw cycles.

运输条件

Shipping with blue ice.

验证图片

ICC

Immunofluorescence analysis of MCF7 cells labeling Cytokeratin 19 with Cytokeratin 19 (HY-P81250) at 1/100 dilution. Cells were fixed in 4% paraformaldehyde for 20 minutes at room temperature, permeabilized with 0.1% Triton X-100 in PBS for 5 minutes at room temperature, then blocked with 1% BSA/10% normal goat serum for 1 hour at room temperature. Cells were then incubated with Cytokeratin 19 (HY-P81250) at 1/100 dilution in 1% BSA in PBST overnight at 4 ℃. Alexa Fluor® 647-conjugated AffiniPure Goat Anti-Mouse IgG H&L (HY-P81013) was used as the secondary antibody at 1/1,000 dilution. Nuclear DNA was labelled in blue with DAPI.
Immunofluorescence analysis of HEC1A cells labeling Cytokeratin 19 with Cytokeratin 19 (HY-P81250) at 1/100 dilution. Cells were fixed in 4% paraformaldehyde for 20 minutes at room temperature, permeabilized with 0.1% Triton X-100 in PBS for 5 minutes at room temperature, then blocked with 1% BSA/10% normal goat serum for 1 hour at room temperature. Cells were then incubated with Cytokeratin 19 (HY-P81250) at 1/100 dilution in 1% BSA in PBST overnight at 4 ℃. Alexa Fluor® 647-conjugated AffiniPure Goat Anti-Mouse IgG H&L (HY-P81013) was used as the secondary antibody at 1/1,000 dilution. Nuclear DNA was labelled in blue with DAPI.

文件资料

Select Batch:

Data Sheet (438 KB) SDS (251 KB)

COA (193 KB)

抗体使用指南 (1083 KB)

Alexa Fluor® 647-conjugated AffiniPure Goat Anti-Mouse IgG H&L 相关分类

Help & FAQs

Do most proteins show cross-species activity?
Species cross-reactivity must be investigated individually for each product. Many human cytokines will produce a nice response in mouse cell lines, and many mouse proteins will show activity on human cells. Other proteins may have a lower specific activity when used in the opposite species.

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MedChemExpress (MCE) 只为有资质的科研机构、医药企业基于科学研究或药证申报的用途提供医药研发服务，不为任何个人或者非科研性质的、非用于药证申报使用等其他用途提供服务。

站点地图隐私声明

沪ICP备15051369号-4

上海工商

沪公网安备31011502019417

沪(浦)应急管危经许[2021]201709(QFYS)

营业执照（三证合一）

Alexa Fluor® 647-conjugated AffiniPure Goat Anti-Mouse IgG H&L

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