ATG5 抗体 (YA599)

目录号: HY-P80022: Data Sheet SDS COA 抗体使用指南
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ATG5 Antibody (YA599) 是一个兔来源、无偶联标记、抗 ATG5 的 IgG 单克隆抗体。

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规格	价格	是否有货
10 μL	￥470	In-stock
50 μL	￥1220	In-stock
100 μL	￥2000	In-stock
250 μL		询价

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ATG5 Antibody (YA599) 相关产品:

WB: 蛋白质免疫印迹;
IHC-P: 石蜡切片样本的免疫组织化学;
IHC-F: 冰冻切片样本的免疫组织化学;
ICC/IF: 细胞免疫荧光;
IF-Tissue: 组织免疫荧光;
mIHC: 多重荧光免疫组化;
IP: 免疫沉淀;
ChIP: 染色质免疫沉淀;
FC: 流式细胞术;
ELISA: 酶联免疫吸附试验

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描述

ATG5 Antibody (YA599) is a Rabbit-derived and non-conjugated IgG monoclonal antibody, targeting to ATG5.

宿主

Rabbit

克隆性

Recombinant, Monoclonal

分子量

Predicted band size: 32 kDa;

Observed band size: 55 kDa

请注意：因蛋白存在修饰或聚体等情况，以实测为准，预测仅为参考。

反应种属

Human, Rat, Mouse, Monkey

蛋白数据库

Q9H1Y0

基因 ID

9474 [NCBI]

免疫原

Synthetic peptide corresponding to Human ATG5.AA range:1-50.

应用 & 推荐
稀释比例

应用	稀释比
WB WB: 蛋白质免疫印迹	1:1,000-1:5,000
ICC/IF ICC/IF: 细胞免疫荧光	1:50-1:200
IHC-P IHC-P: 石蜡切片样本的免疫组织化学	1:50-1:100
IP IP: 免疫沉淀	1-2μg/sample
FC FC: 流式细胞术	1:1,000
IF-Tissur	1:50-1:200

敏感性	Endogenous	纯度	Protein A affinity purified.
偶联	Non-conjugated	修饰	Unmodified
同型	IgG

性状

液体

组分

Supplied in 1*TBS (pH7.4), 0.05% BSA and 40% Glycerol. Preservative: 0.05% Sodium Azide.

保存条件 & 期限

Stored at -20°C for 1 year. Avoid repeated freeze / thaw cycles.

运输条件

Shipping with blue ice.

验证图片

ALL WB ICC

Western blot analysis of extracts from NIH3T3(lane 2(20μg) , C2C12 (lane 3(20μg), Hela (lane 4(20μg) and HEK293(lane 5(20μg) using ATG5 (HY-P80022) Rabbit mAb. Proteins were transferred to a PVDF membrane and blocked with 5% non-fat milk in TBST for 2 hour at room temperature. The primary antibody (1/1000) and Loading control antibody (Beta Actin, HY-P80438, 1/10000) was used in 5% non-fat milk in TBST at 4°C overnight. Goat Anti-Mouse IgG-HRP Secondary Antibody (1/10000) was used for 1 hour at room temperature.
Immunocytochemistry analysis of NIH/3T3 cells labeling ATG5 with ATG5 Antibody (HY-P80022)at 1/50 dilution. Cells were fixed in 4% paraformaldehyde for 15 minutes at room temperature, permeabilized with 0.1% Triton X-100 for 10 minutes at room temperature, then blocked with QuickBlock™ Blocking Buffer for Immunol Staining for 10 min at room temperature. Cells were then incubated with ATG5 Antibody (HY-P80022) at 1/50 dilution in QuickBlock™ Blocking Buffer for Immunol Staining at 4 ℃. Alexa Fluor® 488-conjugated AffiniPure Goat Anti-Rabbit IgG H&L（HY-P8002, Green) was used as the secondary antibody at 1/1,000 dilution. PBS instead of the primary antibody was used as the secondary antibody only control. The Nuclear counterstain was DAPI (Blue).
Immunocytochemistry analysis of NIH/3T3 cells labeling ATG5 with ATG5 Antibody (HY-P80022) at 1/100 dilution. Cells were fixed in 4% paraformaldehyde for 15 minutes at room temperature, permeabilized with 0.1% Triton X-100 for 10 minutes at room temperature, then blocked with QuickBlock™ Blocking Buffer for Immunol Staining for 10 min at room temperature. Cells were then incubated with ATG5 Antibody (HY-P80022) at 1/100 dilution in QuickBlock™ Blocking Buffer for Immunol Staining at 4 ℃. Alexa Fluor® 488-conjugated AffiniPure Goat Anti-Rabbit IgG H&L（HY-P8002, Green) was used as the secondary antibody at 1/1,000 dilution. PBS instead of the primary antibody was used as the secondary antibody only control. The Nuclear counterstain was DAPI (Blue).

RRID

AB_3102265

反应种属数据库

Entrez Gene: 9474 Human ; 11793 Mouse ; 365601 Rat

SwissProt: Q9H1Y0 Human ; Q99J83 Mouse ; Q3MQ06 Rat

OMIM: 617584 Human

研究领域

Cell Biology

中文名

ATG5 抗体

同用名

APG 5 antibody; APG 5L antibody; APG5 antibody; APG5 autophagy 5 like antibody; APG5 like antibody; APG5-like antibody; APG5L antibody; Apoptosis specific protein antibody; Apoptosis-specific protein antibody; ASP antibody; ATG 5 antibody; Atg5 antibody; ATG5 autophagy related 5 homolog antibody; ATG5_HUMAN antibody; Autophagy protein 5 antibody; Autophagy related 5 antibody; hAPG5 antibody; Homolog of S Cerevisiae autophagy 5 antibody; OTTHUMP00000040507 antibody

文件资料

Select Batch:

Data Sheet (443 KB) SDS (251 KB)

COA (193 KB)

抗体使用指南 (1083 KB)

ATG5 Antibody (YA599) 相关分类

Help & FAQs

Do most proteins show cross-species activity?
Species cross-reactivity must be investigated individually for each product. Many human cytokines will produce a nice response in mouse cell lines, and many mouse proteins will show activity on human cells. Other proteins may have a lower specific activity when used in the opposite species.

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上海工商

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沪(浦)应急管危经许[2021]201709(QFYS)

营业执照（三证合一）

ATG5 抗体 (YA599)

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