Calnexin 抗体 (YA573)

Calnexin Antibody (YA573) is a Rabbit-derived and non-conjugated IgG monoclonal antibody, targeting to Calnexin.

钙连接蛋白是一种位于内质网 (ER) 中的钙结合伴侣，在与新合成的单葡萄糖基化糖蛋白相互作用中发挥着关键作用，有助于蛋白质组装和未组装蛋白质亚基在 ER 内的保留。钙连接蛋白在 ER 质量控制装置中发挥着关键作用，参与保留错误折叠的蛋白质。值得注意的是，它与未成熟胸腺细胞中的部分 T 细胞抗原受体复合物相关，表明在胸腺细胞成熟的信号传导和调节中发挥作用。此外，钙连接蛋白可能参与突触受体介导的内吞作用。其相互作用扩展到多种蛋白质，包括 MAPK3/ERK1、KCNH2、SERPINA2P/SERPINA2、SGIP1、PPIB、SMIM22、TMX2、TMEM35A/NACHO、CHRNA7、RETREG2、RETREG3、DNM1L 和 ADAM7，突出了其在形成复合物和参与不同的细胞过程。棕榈酰化形式与核糖体易位子复合物成分 SSR1 特异性相互作用，促进糖蛋白的有效折叠并强调其在内质网内蛋白质成熟中的作用。

Free

P27824

Predicted band size: 68 kDa；Observed band size: 100 kDa

Protein A affinity purified.

Endoplasmic reticulum membrane, Endoplasmic reticulum, Melanosome.

Non-conjugated

Unmodified

AB_3102277

Tags & Cell Markers

Primary Antibody; Recombinant Rabbit Monoclonal Antibody

Recombinant, Monoclonal

Rabbit

Human, Rat

WB: 1:1,000-1:5,000 ;ICC/IF: 1:100-1:500 ;IHC-P: 1:50-1:200 ;FC: 1:50-1:100

• 
  Western blot analysis of extracts from Hela (lane 2(20μg) and Hela (lane 3(40μg)，HepG2 (lane 4(20μg) ) and HepG2 (lane 5(40μg) )using Calnexin (HY-P80041) Rabbit mAb. Proteins were transferred to a PVDF membrane and blocked with 5% BSA in TBST for 2 hour at room temperature. The primary antibody (HY-P80041, 1/1000) and Loading control antibody (Beta Actin, HY-P80438, 1/3000) was used in 5% BSA in TBST at 4°C overnight. Goat Anti-Mouse/Rabbit IgG-HRP Secondary Antibody (HY-P8004/HY-P8001, 1/10,000) was used for 1 hour at room temperature.
• 
  Immunohistochemical analysis of paraffin-embedded Mouse liver tissue using Calnexin Antibody (YA573). The section was pre-treated using heat mediated antigen retrieval with sodium citrate buffer (pH 6.0) for 8 minutes. The tissues were blocked in QuickBlock for 20 minutes at room temperature, washed with ddH2O and PBS, and then probed with the primary antibody (HY-P80041, 1/1000) in 4℃ overnight. The detection was performed using an HRP conjugated compact polymer system. DAB was used as the chromogen. Tissues were counterstained with hematoxylin and mounted with DPX.
• 
  Immunohistochemical analysis of paraffin-embedded Mouse liver tissue using Calnexin Antibody (YA573). The section was pre-treated using heat mediated antigen retrieval with sodium citrate buffer (pH 6.0) for 8 minutes. The tissues were blocked in QuickBlock for 20 minutes at room temperature, washed with ddH2O and PBS, and then probed with the primary antibody (HY-P80041, 1/1000) in 4℃ overnight. The detection was performed using an HRP conjugated compact polymer system. DAB was used as the chromogen. Tissues were counterstained with hematoxylin and mounted with DPX.

WB, ICC/IF, IHC-P, FC

液体

Supplied in 1*TBS (pH7.4), 0.05% BSA and 40% Glycerol. Preservative: 0.05% Sodium Azide.

Stored at -20°C for 1 year. Avoid repeated freeze / thaw cycles.

Shipping with blue ice.

IgG

Endogenous

Synthetic peptide corresponding to Human Calnexin.AA range:543-592.