CD133 抗体 (YA808)

CD133 Antibody is a non-conjugated and Mouse origined monoclonal antibody about 97 kDa, targeting to CD133. It can be used for WB,IHC-P,FC assays with tag free, in the background of Human.

Free

O43490

Predicted band size: 97 kDa;Observed band size: 120 kDa

Protein G affinity purified.

Endoplasmic reticulum. Plasma membrane. Cell projection.

Non-conjugated

Unmodified

AB_3102063

Neuroscience

Primary Antibody; Mouse Monoclonal Antibody

Monoclonal

Mouse

Human

WB: 1:500 ;IHC-P: 1:600 ;FC: 1:500-1:1,000

• 
  Western blot analysis of extracts from HT-29 (lane 2(20μg) and HT-29 (lane 3(40μg)using CD133 (HY-P80054) Rabbit mAb. Proteins were transferred to a PVDF membrane and blocked with 5% defatted milk powder in TBST for 2 hour at room temperature. The primary antibody (HY-P80054, 1/1000) and Loading control antibody (Beta Actin, HY-P80438, 1/3000) was used in 5% defatted milk powder in TBST at 4°C overnight. Goat Anti-Mouse/Rabbit IgG-HRP Secondary Antibody (HY-P8004/HY-P8001, 1/10,000) was used for 1 hour at room temperature.

WB, IHC-P, FC

液体

Supplied in 1*PBS (pH7.4), 0.1% BSA, 40% Glycerol. Preservative: 0.05% Sodium Azide.

Stored at -20°C for 1 year. Avoid repeated freeze / thaw cycles.

Shipping with blue ice.

IgG1

Endogenous

Synthetic peptide corresponding to Human CD133.AA range:151-400.