CEBP beta 抗体 (YA1835)

目录号: HY-P82090: Data Sheet SDS COA 抗体使用指南
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CEBP beta Antibody (YA1835) 是一个兔来源、无偶联标记、抗 CEBP beta 的 IgG 单克隆抗体。

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规格	价格	是否有货
10 μL	￥470	In-stock
50 μL	￥1220	In-stock
100 μL	￥2000	In-stock
250 μL		询价

or 大包装询价

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CEBP beta Antibody (YA1835) 相关产品:

WB: 蛋白质免疫印迹;
IHC-P: 石蜡切片样本的免疫组织化学;
IHC-F: 冰冻切片样本的免疫组织化学;
ICC/IF: 细胞免疫荧光;
IF-Tissue: 组织免疫荧光;
mIHC: 多重荧光免疫组化;
IP: 免疫沉淀;
ChIP: 染色质免疫沉淀;
FC: 流式细胞术;
ELISA: 酶联免疫吸附试验

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描述

CEBP beta Antibody (YA1835) is a Rabbit-derived and non-conjugated IgG monoclonal antibody, targeting to CEBP beta.

宿主

Rabbit

克隆性

Recombinant, Monoclonal

分子量

Predicted band size: 36 kDa;

Observed band size: 36 kDa

请注意：因蛋白存在修饰或聚体等情况，以实测为准，预测仅为参考。

反应种属

Human, Mouse, Rat

蛋白数据库

P17676

基因 ID

1051 [NCBI]

免疫原

A synthesized peptide derived from human CEBP Beta aa300-345/345.

应用 & 推荐
稀释比例

应用	稀释比
WB WB: 蛋白质免疫印迹	1:500-1:1000
ICC/IF ICC/IF: 细胞免疫荧光	1:50-1:200
IP IP: 免疫沉淀	1:50
FC FC: 流式细胞术	1:50-1:100

敏感性	Endogenous	纯度	Affinity Chromatography
偶联	Non-conjugated	修饰	Unmodified
同型	IgG

性状

液体

组分

Supplied in Rabbit IgG in 10mM phosphate buffered saline , pH 7.4, 150mM sodium chloride, 0.05% BSA, 0.02% sodium azide and 50% glycerol.

保存条件 & 期限

Stored at -20°C for 1 year. Avoid repeated freeze / thaw cycles.

运输条件

Shipping with blue ice.

验证图片

ALL WB ICC

Western blot analysis of extracts from NIH/3T3(lane 2(20ug) and NIH/3T3(lane 3(40ug) using CEBPB Antibody (HY-P82090) Rabbit mAb. Proteins were transferred to a PVDF membrane and blocked with 5% non-fat milk in TBST for 2 hour at room temperature. The primary antibody (1/1000) and Loading control antibody (Beta Actin, HY-P80438, 1/10000) was used in 5% non-fat milk in TBST at 4°C overnight. Goat Anti-Mouse/Rabbit IgG-HRP Secondary Antibody (1/10000) was used for 1 hour at room temperature.
Immunocytochemistry analysis of NIH-3T3 cells labeling CEBPB with CEBPB antibody (HY-P82090) at 1/50 dilution. Cells were fixed in 4% paraformaldehyde for 15 minutes at room temperature, permeabilized with 0.1% Triton X-100 for 10 minutes at room temperature, then blocked with QuickBlock™ Blocking Buffer for Immunol Staining for 10 min at room temperature. Cells were then incubated with CEBPB antibody (HY-P82090) at 1/50 dilution in QuickBlock™ Blocking Buffer for Immunol Staining at 4 ℃. Alexa Fluor® 488-conjugated AffiniPure Goat Anti-Rabbit IgG H&L（HY-P8002, Green) was used as the secondary antibody at 1/1,000 dilution. PBS instead of the primary antibody was used as the secondary antibody only control. The Nuclear counterstain was DAPI (Blue).
Immunocytochemistry analysis of HeLa cells labeling CEBPB with CEBPB antibody (HY-P82090) at 1/50 dilution. Cells were fixed in 4% paraformaldehyde for 15 minutes at room temperature, permeabilized with 0.1% Triton X-100 for 10 minutes at room temperature, then blocked with QuickBlock™ Blocking Buffer for Immunol Staining for 10 min at room temperature. Cells were then incubated with CEBPB antibody (HY-P82090) at 1/50 dilution in QuickBlock™ Blocking Buffer for Immunol Staining at 4 ℃. Alexa Fluor® 488-conjugated AffiniPure Goat Anti-Rabbit IgG H&L（HY-P8002, Green) was used as the secondary antibody at 1/1,000 dilution. PBS instead of the primary antibody was used as the secondary antibody only control. The Nuclear counterstain was DAPI (Blue).

RRID

AB_3104025

反应种属数据库

Entrez Gene: 1051 Human ; 12608 Mouse ; 24253 Rat

SwissProt: P17676 Human ; P28033 Mouse ; P21272 Rat

OMIM: 189965 Human

研究领域

Epigenetics and Nuclear Signaling

中文名

CEBP beta 抗体 (YA1835)

同用名

CEBPB; LAP; TCF5; PP9092; CCAAT/enhancer-binding protein beta; C/EBP beta; Liver activator protein; Nuclear factor NF-IL6; Transcription factor 5; TCF-5

文件资料

Select Batch:

Data Sheet (443 KB) SDS (251 KB)

COA (194 KB)

抗体使用指南 (1083 KB)

CEBP beta Antibody (YA1835) 相关分类

Help & FAQs

Do most proteins show cross-species activity?
Species cross-reactivity must be investigated individually for each product. Many human cytokines will produce a nice response in mouse cell lines, and many mouse proteins will show activity on human cells. Other proteins may have a lower specific activity when used in the opposite species.

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CEBP beta 抗体 (YA1835)

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