Connexin 43 抗体

Connexin 43 Antibody is a Rabbit-derived and non-conjugated IgG polyclonal antibody, targeting to Connexin 43.

Connexin 43：该基因是连接蛋白基因家族的成员。编码的蛋白质是间隙连接的一个组成部分，间隙连接由细胞间通道阵列组成，这些通道为低分子量材料从细胞到细胞的扩散提供了途径。编码的蛋白质是心脏间隙连接的主要蛋白质，被认为在心脏同步收缩和胚胎发育中起着至关重要的作用。一个相关的无内含子假基因已被定位到 5 号染色体。该基因的突变与眼齿趾发育不良、常染色体隐性颅骨干骺端发育不良和心脏畸形有关。[由 RefSeq 提供，2014 年 5 月]

Free

P17302

Predicted band size: 43 kDa; Observed band size: 43 kDa

affinity purified

Non-conjugated

Unmodified

AB_3102933

Signal Transduction

Primary Antibody; Rabbit Polyclonal Antibody

Polyclonal

Rabbit

Human, Mouse, Rat

WB: 1:500-1:1000; IHC: 1:50-1:100; IF: 1:50-1:200; ELISA: 1:10000

• 
  Western blot analysis of extracts from Hela(lane 2(20ug) and Hela(lane 3(40ug) using Connexin 43 Antibody (HY-P80628) Rabbit mAb. Proteins were transferred to a PVDF membrane and blocked with 5% non-fat milk in TBST for 2 hour at room temperature. The primary antibody (1/1000) and Loading control antibody (Beta Actin, HY-P80438, 1/10000) was used in 5% non-fat milk in TBST at 4°C overnight. Goat Anti-Mouse/Rabbit IgG-HRP Secondary Antibody (1/10000) was used for 1 hour at room temperature.
• 
  Immunohistochemical analysis of paraffin-embedded mouse testis tissue using Connexin 43 Antibody. The section was pre-treated using heat mediated antigen retrieval with sodium citrate buffer (pH 6.0) for 8 minutes. The tissues were blocked in QuickBlock for 20 minutes at room temperature, washed with ddH2O and PBS, and then probed with the primary antibody at 1/100 dilution in 4℃ overnight. The detection was performed using an HRP conjugated compact polymer system. DAB was used as the chromogen. Tissues were counterstained with hematoxylin and mounted with DPX.
• 
  Immunohistochemical analysis of paraffin-embedded mouse testis tissue using Connexin 43 Antibody. The section was pre-treated using heat mediated antigen retrieval with sodium citrate buffer (pH 6.0) for 8 minutes. The tissues were blocked in QuickBlock for 20 minutes at room temperature, washed with ddH2O and PBS, and then probed with the primary antibody at 1/100 dilution in 4℃ overnight. The detection was performed using an HRP conjugated compact polymer system. DAB was used as the chromogen. Tissues were counterstained with hematoxylin and mounted with DPX.

WB, IHC-F, IHC-P, ICC/IF, ELISA

液体

Supplied in 1*PBS (pH 7.3), 50% glycerol and 0.5% BSA. Preservative: 0.02% sodium azide.

Stored at -20°C for 1 year. Avoid repeated freeze / thaw cycles.

Shipping with blue ice.

IgG

Endogenous

Synthetic peptide corresponding to Human Connexin 43.AA range:333-382