Cyclophilin 40 抗体 (YA2099)

Cyclophilin 40 Antibody (YA2099) is a Rabbit-derived and non-conjugated IgG monoclonal antibody, targeting to Cyclophilin 40.

Cyclophilin 40 是一种 PPIase，能够催化寡肽中脯氨酸亚氨基肽键的顺反异构化，从而协助蛋白质折叠。它被提议作为 HSP90 复合物（如未配体类固醇受体异源复合物）中的辅助伴侣蛋白。不同的辅助伴侣蛋白似乎会竞争与 HSP90 结合，从而形成不同的 HSP90-辅助伴侣蛋白-受体复合物，可能实现对受体活性的组织特异性调控。Cyclophilin 40 可能对雌激素受体复合物具有偏好性，但不存在于糖皮质激素受体复合物中。它可能参与细胞质动力蛋白依赖的受体从细胞质到细胞核的转运，并通过抑制 MYB 的 DNA 结合活性来调节其功能。此外，Cyclophilin 40 通过促进 AHR:ARNT 二聚体的形成参与 AHR 信号通路的调控，这一功能不依赖于 HSP90 但需要其伴侣活性。它还参与调控紫外线辐射诱导的细胞凋亡，并在间变性淋巴瘤激酶阳性间变性大细胞淋巴瘤（ALK+ ALCL）细胞系中促进细胞存活。by similar: 可能参与丙型肝炎病毒（HCV）的复制和释放。

Q08752

Predicted band size: 41 kDa; Observed band size: 41 kDa

Affinity Purified

Non-conjugated

Unmodified

AB_3104463

-

Primary Antibody; Recombinant Rabbit Monoclonal Antibody

Recombinant, Monoclonal

Rabbit

Human, Mouse, Rat

WB: 1:500-1:1000; IHC: 1:50-1:100; IF: 1:50-1:200; FC: 1:50-1:100

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  Immunocytochemistry analysis of Hela cells labeling Cyclophilin 40 Antibody (HY-P82354) at 1/50 dilution. Cells were fixed in 4% paraformaldehyde for 15 minutes at room temperature, permeabilized with 0.1% Triton X-100 for 10 minutes at room temperature, then blocked with QuickBlock™ Blocking Buffer for Immunol Staining for 10 min at room temperature. Cells were then incubated with Cyclophilin 40 Antibody (HY-P82354) at 1/50 dilution in QuickBlock™ Blocking Buffer for Immunol Staining at 4 ℃. Alexa Fluor® 488-conjugated AffiniPure Goat Anti-Rabbit IgG H&L（HY-P8002, Green) was used as the secondary antibody at 1/1,000 dilution. PBS instead of the primary antibody was used as the secondary antibody only control. The Nuclear counterstain was DAPI (Blue).
• 
  Immunocytochemistry analysis of HEK293T cells labeling Cyclophilin 40 Antibody (HY-P82354) at 1/50 dilution. Cells were fixed in 4% paraformaldehyde for 15 minutes at room temperature, permeabilized with 0.1% Triton X-100 for 10 minutes at room temperature, then blocked with QuickBlock™ Blocking Buffer for Immunol Staining for 10 min at room temperature. Cells were then incubated with Cyclophilin 40 Antibody (HY-P82354) at 1/50 dilution in QuickBlock™ Blocking Buffer for Immunol Staining at 4 ℃. Alexa Fluor® 488-conjugated AffiniPure Goat Anti-Rabbit IgG H&L（HY-P8002, Green) was used as the secondary antibody at 1/1,000 dilution. PBS instead of the primary antibody was used as the secondary antibody only control. The Nuclear counterstain was DAPI (Blue).
• 
  Immunohistochemical analysis of paraffin-embedded Rat testis tissue using Cyclophilin 40 Antibody (YA2099). The section was pre-treated using heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0) for 8 minutes. The tissues were blocked in QuickBlock for 20 minutes at room temperature, washed with ddH2O and PBS, and then probed with the primary antibody (HY-P82354, 1/100) in 4℃ overnight. The detection was performed using an HRP conjugated compact polymer system. DAB was used as the chromogen. Tissues were counterstained with hematoxylin and mounted with DPX.
• 
  Immunohistochemical analysis of paraffin-embedded Rat testis tissue using Cyclophilin 40 Antibody (YA2099). The section was pre-treated using heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0) for 8 minutes. The tissues were blocked in QuickBlock for 20 minutes at room temperature, washed with ddH2O and PBS, and then probed with the primary antibody (HY-P82354, 1/100) in 4℃ overnight. The detection was performed using an HRP conjugated compact polymer system. DAB was used as the chromogen. Tissues were counterstained with hematoxylin and mounted with DPX.

WB, IHC-P, ICC/IF, FC

液体

Supplied in Rabbit IgG in 10mM phosphate buffered saline , pH 7.4, 150mM sodium chloride, 0.05% BSA, 0.02% sodium azide and 50% glycerol.

Stored at -20°C for 1 year. Avoid repeated freeze / thaw cycles.

Shipping with blue ice.

IgG

Endogenous

A synthesized peptide derived from human Cyclophilin 40