Cystathionase 抗体 (YA2452)

Cystathionase Antibody (YA2452) is a Rabbit-derived and non-conjugated IgG monoclonal antibody, targeting to Cystathionase.

Cystathionase 是一种依赖 pyridoxal-5'-phosphate (PLP) 的酶，催化从 L-methionine 到 L-cysteine 的转硫途径的最后一步，即将 L,L-cystathionine 分子裂解为 L-cysteine、氨和 2-oxobutanoate。该酶还利用 L-cysteine 和 L-homocysteine 作为底物（速率远低于 L,L-cystathionine）产生内源性气体信号分子 hydrogen sulfide (H2S)。在体外，它能将两个 L-cysteine 分子转化为 lanthionine 和 H2S，或将两个 L-homocysteine 分子转化为 homolanthionine 和 H2S，这在严重高同型半胱氨酸血症（心血管疾病、糖尿病和阿尔茨海默病的风险因素）条件下尤为重要。此外，Cystathionase 作为 cysteine-protein sulfhydrase，通过介导目标蛋白（如 GAPDH、PTPN1 和 NF-kappa-B 亚基 RELA）的硫化作用（将 -SH 基团转化为 -SSH）来调节其功能。通过生成气体递质 H2S，它参与多种生理过程，如血管舒张、骨骼保护和炎症。在骨骼肌组织中，它对 H2S 的生物合成起重要作用（by similar）。该酶还能以 homoserine 为底物（by similar），并催化 selenocystathionine（可来源于饮食）的消除，生成 selenocysteine、氨和 2-oxobutanoate（by similar）。

P32929

Predicted band size: 45 kDa; Observed band size: 42 kDa

Affinity Purified

Non-conjugated

Unmodified

AB_3104806

Signal Transduction

Primary Antibody; Recombinant Rabbit Monoclonal Antibody

Recombinant, Monoclonal

Rabbit

Human

WB: 1:500-1:1000; IP: 1:50

• 
  Western blot analysis of extracts from HepG2(lane 2(20ug) , HEK293T(lane 3(20ug) and K562(lane 4(20ug) using Cystathionase Antibody(HY-P82707) Rabbit mAb. Proteins were transferred to a PVDF membrane and blocked with 5% non-fat milk in TBST for 2 hour at room temperature. The primary antibody (1/1000) and Loading control antibody (Beta Actin, HY-P80438, 1/10000) was used in 5% non-fat milk in TBST at 4°C overnight. Goat Anti-Mouse/Rabbit IgG-HRP Secondary Antibody (1/10000) was used for 1 hour at room temperature.

WB, IP

液体

Supplied in 50mM Tris-Glycine(pH 7.4), 0.15M NaCl, 40%Glycerol, 0.01% sodium azide and 0.05% BSA.

Stored at -20°C for 1 year. Avoid repeated freeze / thaw cycles.

Shipping with blue ice.

IgG

Endogenous

A synthesized peptide derived from human Cystathionase