Cytokeratin 19 抗体

Cytokeratin 19 Antibody is a Rabbit-derived and non-conjugated IgG polyclonal antibody, targeting to Cytokeratin 19.

Cytokeratin 是一种参与肌纤维组织的蛋白质，它与 KRT8 共同作用，帮助将收缩装置连接到横纹肌 costameres 处的 dystrophin 蛋白。

P08727

Predicted band size: 42 kDa; Observed band size: 42 kDa

affinity purified

Non-conjugated

Unmodified

AB_3103090

Primary Antibody; Rabbit Polyclonal Antibody

Polyclonal

Rabbit

Human

Mouse, Rat, Chicken, Pig, Cow, Dog, Horse

WB: 1:500-2000; ELISA: 1:5000-10000; IHC-P: 1:100-500; IHC-F: 1:100-500; FC: 1μg/test; IF: 1:100-500

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  Immunofluorescence analysis of MCF7 cells labeling Cytokeratin 19 with Cytokeratin 19 (HY-P81183) at 1/100 dilution. Cells were fixed in 4% paraformaldehyde for 20 minutes at room temperature, permeabilized with 0.1% Triton X-100 in PBS for 5 minutes at room temperature, then blocked with 1% BSA/10% normal goat serum for 1 hour at room temperature. Cells were then incubated with Cytokeratin 19 (HY-P81183) at 1/100 dilution in 1% BSA in PBST overnight at 4 ℃. Alexa Fluor® 647-conjugated AffiniPure Goat Anti-Rabbit IgG H&L (HY-P80952) was used as the secondary antibody at 1/1,000 dilution. Nuclear DNA was labelled in blue with DAPI.
• 
  Immunofluorescence analysis of HEC1A cells labeling Cytokeratin 19 with Cytokeratin 19 (HY-P81183) at 1/100 dilution. Cells were fixed in 4% paraformaldehyde for 20 minutes at room temperature, permeabilized with 0.1% Triton X-100 in PBS for 5 minutes at room temperature, then blocked with 1% BSA/10% normal goat serum for 1 hour at room temperature. Cells were then incubated with Cytokeratin 19 (HY-P81183) at 1/100 dilution in 1% BSA in PBST overnight at 4 ℃. Alexa Fluor® 647-conjugated AffiniPure Goat Anti-Rabbit IgG H&L (HY-P80952) was used as the secondary antibody at 1/1,000 dilution. Nuclear DNA was labelled in blue with DAPI.

WB, ELISA, IHC-P, IHC-F, FC, ICC/IF

液体

Supplied in 0.01M TBS(pH7.4) with 1% BSA, 0.03% Proclin300 and 50% Glycerol.

Stored at -20°C for 1 year. Avoid repeated freeze / thaw cycles.

Shipping with blue ice.

IgG

Endogenous

KLH conjugated synthetic peptide derived from human Cytokeratin 19.: 101-200/403