Estrogen Receptor alpha 抗体 (YA450)

Estrogen Receptor alpha Antibody is a non-conjugated and Rabbit origined monoclonal antibody about 66 kDa, targeting to Estrogen Receptor alpha. It can be used for WB,IHC-F,IHC-P,ICC/IF,IP,ChIP assays with tag free, in the background of Human.

Estrogen Receptor alpha：该基因编码雌激素受体和配体激活的转录因子。经典蛋白包含一个 N 末端配体独立的反式激活结构域、一个中心 DNA 结合结构域、一个铰链结构域和一个 C 末端配体依赖性反式激活结构域。该蛋白质定位于细胞核，在那里它可能与雌激素受体 2 形成同二聚体或异二聚体。该基因编码的蛋白质调节许多雌激素诱导基因的转录，这些基因在生长、新陈代谢、性发育、妊娠、和其他生殖功能，并在许多非生殖组织中表达。由该基因编码的受体在乳腺癌、子宫内膜癌和骨质疏松症中起着关键作用。据报道，由于使用替代启动子和可变剪接，该基因具有数十种转录本变体，但是，其中许多变体的全长性质仍不确定。[由 RefSeq 提供，2020 年 7 月]

Free

P03372

Predicted band size: 66 kDa; Observed band size: 66 kDa

affinity purified

Non-conjugated

Unmodified

AB_3102157

Signal Transduction

Primary Antibody; Recombinant Rabbit Monoclonal Antibody

Recombinant, Monoclonal

Rabbit

Human

WB: 1:500-1:1,000 ;IHC: 1:50-1:100 ;IF: 1:50-1:200 ;IP: 1:20 ;ChIP: 1:20

• 
  Western blot analysis of extracts from K562(lane 2(20μg), Hela (lane 3(20μg) and HEK293T(lane 4(20μg) using Estrogen Receptor alpha (HY-P80663) Rabbit mAb. Proteins were transferred to a PVDF membrane and blocked with 5% non-fat milk in TBST for 2 hour at room temperature. The primary antibody (1/1000) and Loading control antibody (Beta Actin, HY-P80438, 1/10000) was used in 5% non-fat milk in TBST at 4°C overnight. Goat Anti-Mouse/Rabbit IgG-HRP Secondary Antibody (1/10000) was used for 1 hour at room temperature.

WB, IHC-F, IHC-P, ICC/IF, IP, ChIP

液体

Supplied in 50 mM Tris-Glycine (pH 7.4), 0.15 M NaCl, 40% Glycerol and 0.05% BSA. Preservative: 0.01% Sodium azide

Stored at -20°C for 1 year. Avoid repeated freeze / thaw cycles.

Shipping with blue ice.

IgG

Endogenous

Synthetic peptide corresponding to Human Estrogen Receptor alpha.The exact sequence is proprietary to MCE.