FADS1 抗体 (YA2910)

FADS1 Antibody (YA2910) is a Rabbit-derived and non-conjugated IgG monoclonal antibody, targeting to FADS1.

FADS1 是一种前端脂肪酰辅酶 A (CoA) 去饱和酶，能够在脂肪酰链的羧基端与预先存在的双键之间的第 5 位碳原子上引入一个顺式双键。它参与从必需多不饱和脂肪酸 (PUFA) 亚油酸 (LA，18:2n-6) 和 α-亚麻酸 (ALA，18:3n-3) 前体合成高度不饱和脂肪酸 (HUFA)。具体而言，FADS1 将二高-γ-亚麻酸 (DGLA，20:3n-6) 和二十碳四烯酸 (ETA，20:4n-3) 去饱和，分别生成花生四烯酸 (AA，20:4n-6) 和二十碳五烯酸 (EPA，20:5n-3)。作为 DGLA (20:3n-6) 和 AA (20:4n-6) 衍生类二十烷酸生物合成的限速酶，FADS1 控制着前列腺素 E2 等炎症脂质的代谢，对有效的急性炎症反应和上皮稳态的维持至关重要。此外，FADS1 通过提供 AA (20:4n-6) 作为酯化到磷脂中的主要酰基链，参与膜磷脂的生物合成。特别值得注意的是，它调节磷脂酰肌醇-4,5-二磷酸的水平，从而调控 T 细胞中炎性细胞因子的产生 (by similarity)。FADS1 还能去饱和 (11E)-十八碳烯酸 (反式异油酸，18:1n-9)，这是 LA (18:2n-6) 生物氢化途径中的一种代谢物 (by similarity)。需要注意的是，FADS1 对 20:3n-6 没有催化活性，但可能增强 FADS2 的活性。

O60427

Predicted band size: 52 kDa; Observed band size: 52 kDa

Affinity Purified

Non-conjugated

Unmodified

AB_3105280

Tags & Cell Markers

Primary Antibody; Recombinant Rabbit Monoclonal Antibody

Recombinant, Monoclonal

Rabbit

Human, Mouse, Rat

WB: 1:500-1:1000; IHC: 1:50-1:100; IF: 1:50-1:200

• 
  Western blot analysis of extracts from HepG2(lane 2(20ug) and HepG2(lane 3(40ug) using FADS1 Antibody (HY-P83165) Rabbit mAb. Proteins were transferred to a PVDF membrane and blocked with 5% non-fat milk in TBST for 2 hour at room temperature. The primary antibody (1/1000) and Loading control antibody (Beta Actin, HY-P83730, 1/10000) was used in 5% non-fat milk in TBST at 4°C overnight. Goat Anti-Mouse/Rabbit IgG-HRP Secondary Antibody (1/10000) was used for 1 hour at room temperature.
• 
  Immunocytochemistry analysis of Hela cells labeling FADS1 Antibody Antibody (HY-P83165) at 1/50 dilution. Cells were fixed in 4% paraformaldehyde for 15 minutes at room temperature, permeabilized with 0.1% Triton X-100 for 10 minutes at room temperature, then blocked with QuickBlock™ Blocking Buffer for Immunol Staining for 10 min at room temperature. Cells were then incubated with FADS1 Antibody (HY-P83165) at 1/50 dilution in QuickBlock™ Blocking Buffer for Immunol Staining at 4 ℃. Alexa Fluor® 488-conjugated AffiniPure Goat Anti-Rabbit IgG H&L（HY-P8002, Green) was used as the secondary antibody at 1/1,000 dilution. PBS instead of the primary antibody was used as the secondary antibody only control. The Nuclear counterstain was DAPI (Blue).
• 
  Immunocytochemistry analysis of NIH/3T3 cells labeling FADS1 Antibody Antibody (HY-P83165) at 1/50 dilution. Cells were fixed in 4% paraformaldehyde for 15 minutes at room temperature, permeabilized with 0.1% Triton X-100 for 10 minutes at room temperature, then blocked with QuickBlock™ Blocking Buffer for Immunol Staining for 10 min at room temperature. Cells were then incubated with FADS1 Antibody Antibody (HY-P83165) at 1/50 dilution in QuickBlock™ Blocking Buffer for Immunol Staining at 4 ℃. Alexa Fluor® 488-conjugated AffiniPure Goat Anti-Rabbit IgG H&L（HY-P8002, Green) was used as the secondary antibody at 1/1,000 dilution. PBS instead of the primary antibody was used as the secondary antibody only control. The Nuclear counterstain was DAPI (Blue).

WB, IHC-F, IHC-P, ICC/IF

液体

Supplied in 50mM Tris-Glycine(pH 7.4), 0.15M NaCl, 40% Glycerol, 0.01% Sodium azide and 0.05% BSA

Stored at -20°C for 1 year. Avoid repeated freeze / thaw cycles.

Shipping with blue ice.

IgG

Endogenous

A synthetic peptide of human FADS1 aa101-114.