FMRP 抗体 (YA850)

FMRP Antibody (YA850) is a Rabbit-derived and non-conjugated IgG monoclonal antibody, targeting to FMRP.

FMR1 是一种多功能 polyribosome 相关的 RNA 结合蛋白，在神经元发育和突触可塑性中发挥核心作用，通过调控 mRNA 的可变剪接、稳定性、树突运输以及突触后局部蛋白质合成实现功能。它通过介导相分离形成无膜细胞器，将 mRNA 组装成胞质核糖核蛋白颗粒，富集 mRNA 及其调控因子。FMR1 可稳定海马神经元中的 DLG4/PSD-95 和胶质细胞中的 MBP mRNA，并在代谢型谷氨酸受体 (mGluR) 激活时进一步增强稳定性 (by similar)。它还能选择性递送部分树突 mRNA 至突触位点 (by similar)，并通过磷酸化与 CAPRIN1 相互作用促进翻译抑制相关颗粒的形成。FMR1 作为翻译抑制因子，可阻滞核糖体易位 (by similar)，并参与 miRNA 介导的翻译抑制。同时，它也能激活部分突触 mRNA 的翻译 (by similar)。FMR1 结合大量树突 mRNA，识别 5'-ACU[GU]-3' 或 5'-[AU]GGA-3' 等 RNA 共识序列及 G-quadruplex 结构。此外，它参与 mRNA 核输出，特异性识别 m6A 修饰的 mRNA 并通过 XPO1/CRM1 依赖的方式促进其输出。FMR1 还调控离子通道活性 (如 KCNT1、CACNA1B) 和微管动力学 (by similar)，并可能影响 DNA 损伤响应 (DDR)。在病毒感染中，它正调控流感 A 病毒 (IAV) 复制。

Free

Q06787

Predicted band size: 71 kDa; Observed band size: 71-75 kDa

Protein A affinity purified.

Cell junction, Cell membrane, Cell projection, Centromere, Chromosome, Cytoplasm, Membrane, Nucleus, Postsynaptic cell membrane, Synapse, Synaptosome.

Non-conjugated

Unmodified

AB_3102777

Neuroscience

Primary Antibody; Recombinant Rabbit Monoclonal Antibody

Recombinant, Monoclonal

Rabbit

Human, Mouse, Rat

WB: 1:1000-1:5000; ICC: 1:100-1:500; IF-Tissue: 1:100-1:500; IHC-P: 1:50-1:1000

• 
  Western blot analysis of extracts from K562(lane 2(20μg) and K562(lane 3(40μg) using FMRP(HY-P80970 Rabbit mAb. Proteins were transferred to a PVDF membrane and blocked with 5% non-fat milk in TBST for 2 hour at room temperature. The primary antibody (1/1000) and Loading control antibody (Beta Actin, HY-P80438, 1/10000) was used in 5% non-fat milk in TBST at 4°C overnight. Goat Anti-Mouse/Rabbit IgG-HRP Secondary Antibody (1/10000) was used for 1 hour at room temperature.
• 
  Immunocytochemistry analysis of NIH3T3 cells labeling FMRP with FMRP antibody (HY-P80970) at 1/100 dilution. Cells were fixed in 4% paraformaldehyde for 15 minutes at room temperature, permeabilized with 0.1% Triton X-100 for 10 minutes at room temperature, then blocked with QuickBlock™ Blocking Buffer for Immunol Staining for 10 min at room temperature. Cells were then incubated with FMRP antibody (HY-P80970) at 1/100 dilution in QuickBlock™ Blocking Buffer for Immunol Staining at 4 ℃. Alexa Fluor® 488-conjugated AffiniPure Goat Anti-Rabbit IgG H&L（HY-P8002, Green) was used as the secondary antibody at 1/1,000 dilution. PBS instead of the primary antibody was used as the secondary antibody only control. The Nuclear counterstain was DAPI (Blue).
• 
  Immunocytochemistry analysis of NIH3T3 cells labeling FMRP with FMRP antibody (HY-P80970) at 1/250 dilution. Cells were fixed in 4% paraformaldehyde for 15 minutes at room temperature, permeabilized with 0.1% Triton X-100 for 10 minutes at room temperature, then blocked with QuickBlock™ Blocking Buffer for Immunol Staining for 10 min at room temperature. Cells were then incubated with FMRP antibody (HY-P80970) at 1/250 dilution in QuickBlock™ Blocking Buffer for Immunol Staining at 4 ℃. Alexa Fluor® 488-conjugated AffiniPure Goat Anti-Rabbit IgG H&L（HY-P8002, Green) was used as the secondary antibody at 1/1,000 dilution. PBS instead of the primary antibody was used as the secondary antibody only control. The Nuclear counterstain was DAPI (Blue).
• 
  Immunohistochemical analysis of paraffin-embedded Mouse brain tissue using FMRP Antibody. The section was pre-treated using heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0) for 8 minutes. The tissues were blocked in QuickBlock for 20 minutes at room temperature, washed with ddH2O and PBS, and then probed with the primary antibody (HY-P80970, 1/400) in 4℃ overnight. The detection was performed using an HRP conjugated compact polymer system. DAB was used as the chromogen. Tissues were counterstained with hematoxylin and mounted with DPX.
• 
  Immunohistochemical analysis of paraffin-embedded Mouse brain tissue using FMRP Antibody. The section was pre-treated using heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0) for 8 minutes. The tissues were blocked in QuickBlock for 20 minutes at room temperature, washed with ddH2O and PBS, and then probed with the primary antibody (HY-P80970, 1/400) in 4℃ overnight. The detection was performed using an HRP conjugated compact polymer system. DAB was used as the chromogen. Tissues were counterstained with hematoxylin and mounted with DPX.

WB, ICC/IF, IHC-P

液体

Supplied in 1*TBS (pH7.4), 0.05% BSA, 40% Glycerol. Preservative: 0.05% Sodium Azide.

Stored at -20°C for 1 year. Avoid repeated freeze / thaw cycles.

Shipping with blue ice

IgG

Endogenous

Synthetic peptide within human FMRP aa 20-60.