LOX 1 抗体

LOX 1 Antibody is an unconjugated, approximately 31 kDa, rabbit-derived, anti-LOX 1 polyclonal antibody. LOX 1 Antibody can be used for: WB, IF-Cell, IHC-P, FC expriments in human, mouse background without labeling.

LOX 1 是一种受体，介导血管内皮细胞对氧化修饰低密度脂蛋白 (oxLDL) 的识别、内化和降解。oxLDL 是动脉粥样硬化的标志物，可诱导血管内皮细胞激活和功能障碍，导致促炎反应、促氧化状态和细胞凋亡。其与 oxLDL 的结合通过增加细胞内活性氧的产生和多种促动脉粥样硬化细胞反应（包括减少一氧化氮 (NO) 释放、单核细胞粘附和细胞凋亡）来激活 NF-kappa-B。除了结合 oxLDL 外，它还作为 HSP70 蛋白的受体，参与树突状细胞中抗原交叉呈递给初始 T 细胞的过程，从而参与细胞介导的抗原交叉呈递。此外，它还通过在内毒素诱导的炎症中作为血管界面的白细胞粘附分子参与炎症过程。它还作为晚期糖基化终产物 (AGE)、活化血小板、单核细胞、凋亡细胞以及革兰氏阴性和革兰氏阳性细菌的受体 (By similarity)。

Q9XTA8

Predicted band size: 31 kDa; Observed band size: 50 kDa

affinity purified

Cell membrane. Secreted.

Non-conjugated

Unmodified

AB_3103253

Primary Antibody; Rabbit Polyclonal Antibody

Polyclonal

Rabbit

Human, Rat, Rabbit

Mouse, Cow

WB: 1:500-1:1,000; IF-Cell: 1:50-1:200; IHC-P: 1:50-1:200; FC: 1:50-1:100;

• 
  Western blot analysis of extracts from MCF-7 (lane 2(20μg) , MCF-7 (lane 3(40μg),using LOX 1 Antibody (HY-P81172). Proteins were transferred to a PVDF membrane and blocked with 5% BSA in TBST for 2 hour at room temperature. The primary antibody and Loading control antibody (Beta Actin, HY-P80438, 1/3000) was used in 5% BSA in TBST at 4°C overnight. Goat Anti-Mouse/Rabbit IgG-HRP Secondary Antibody (HY-P8004/HY-P8001, 1/10,000) was used for 1 hour at room temperature.
• 
  Immunocytochemistry analysis of HepG2 cells labeling LOX1 with LOX1 Antibody (HY-P81172) at 1/50 dilution. Cells were fixed in 4% paraformaldehyde for 15 minutes at room temperature, permeabilized with 0.1% Triton X-100 for 10 minutes at room temperature, then blocked with QuickBlock™ Blocking Buffer for Immunol Staining for 10 min at room temperature. Cells were then incubated with LOX1 Antibody (HY-P81172) at 1/50 dilution in QuickBlock™ Blocking Buffer for Immunol Staining at 4 ℃. Alexa Fluor® 488-conjugated AffiniPure Goat Anti-Rabbit IgG H&L（HY-P8002, Green) was used as the secondary antibody at 1/1,000 dilution. PBS instead of the primary antibody was used as the secondary antibody only control. The Nuclear counterstain was DAPI (Blue).
• 
  Immunohistochemical analysis of paraffin-embedded Mouse brain tissue using LOX 1 Antibody. The section was pre-treated using heat mediated antigen retrieval with sodium citrate buffer (pH 6.0) for 8 minutes. The tissues were blocked in QuickBlock for 20 minutes at room temperature, washed with ddH2O and PBS, and then probed with the primary antibody (HY-P81172, 1/400) in 4℃ overnight. The detection was performed using an HRP conjugated compact polymer system. DAB was used as the chromogen. Tissues were counterstained with hematoxylin and mounted with DPX.
• 
  Immunohistochemical analysis of paraffin-embedded Mouse brain tissue using LOX 1 Antibody. The section was pre-treated using heat mediated antigen retrieval with sodium citrate buffer (pH 6.0) for 8 minutes. The tissues were blocked in QuickBlock for 20 minutes at room temperature, washed with ddH2O and PBS, and then probed with the primary antibody (HY-P81172, 1/400) in 4℃ overnight. The detection was performed using an HRP conjugated compact polymer system. DAB was used as the chromogen. Tissues were counterstained with hematoxylin and mounted with DPX.

WB, IHC-P, ICC/IF, FC

MCF-7, Hela, LOVO, human lung tissue lysate, human kidney tissue, human placenta tissue, mouse brain tissue.

液体

Supplied in 1*PBS (pH7.4), 0.2% BSA, 50% Glycerol. Preservative: 0.05% Sodium Azide.

Stored at -20°C for 1 year. Avoid repeated freeze / thaw cycles.

Shipping with blue ice.

IgG

Endogenous

Recombinant protein within Human LOX-1 aa 17-273 / 273.