MCU 抗体 (YA704)

MCU Antibody (YA704) is a Mouse-derived and non-conjugated IgM monoclonal antibody, targeting to MCU.

MCU 蛋白是一种线粒体内膜钙单向转运蛋白，作为单向转运蛋白复合物 (uniplex) 的成孔和钙传导亚基，介导线粒体对钙的摄取。其活性受到 MICU1 和 MICU2 的复杂调节，低 Ca(2+) 水平会下调 MCU 活性，而较高的 Ca(2+) 水平会刺激 MCU 活性。这种调节机制在线粒体钙稳态中发挥着至关重要的作用，影响细胞生理学、生物能量学和各种细胞信号传导途径。在心肌细胞中，MCU 参与缓冲收缩期钙升高，有助于心脏功能。此外，MCU 在急性应激反应中发挥着关键作用，促进起搏细胞中线粒体钙的快速增加。它参与缺血再灌注损伤期间线粒体通透性转变、胰腺β细胞中葡萄糖依赖性胰岛素分泌的调节、成人肌肉大小的调节以及影响中枢神经末梢突触小泡内吞动力学等过程。MCU 的活性会受到钌红或其衍生物 Ru360 等化合物的抑制。

Free

Q8NE86

Predicted band size: 40 kDa, Observed band size: 35 kDa

Protein G affinity purified.

Mitochondrion.

Non-conjugated

Unmodified

AB_3102354

Cardiovascular

Primary Antibody; Mouse Monoclonal Antibody

Monoclonal

Mouse

Human

WB: 1:500-1:1,000; FC: 1:50-1:100

• 
  Western blot analysis of extracts from HEK293(lane 2(20μg) or lane 3(40μg)) and HepG2(lane 4(20μg) or lane 5(40μg)), using MCU (HY-P80216) Mouse mAb. Proteins were transferred to a PVDF membrane and blocked with 5% BSA in TBST for 2 hour at room temperature. The primary antibody (HY-P80216, 1/1000) and Loading control antibody (GAPDH, HY-P80954, 1/3000) was used in 5% BSA in TBST at 4°C overnight. Goat Anti-Mouse/Rabbit IgG-HRP Secondary Antibody (HY-P8004/HY-P8001, 1/10,000) was used for 1 hour at room temperature.

WB, FC

液体

Supplied in 1*PBS (pH7.4), 0.2% BSA and 50% Glycerol. Preservative: 0.05% Sodium Azide.

Stored at -20°C for 1 year. Avoid repeated freeze / thaw cycles.

Shipping with blue ice.

IgM

Endogenous

Synthetic peptide corresponding to Human MCU.AA range:51-325.